{"id":4878,"date":"2025-02-08T13:13:16","date_gmt":"2025-02-08T13:13:16","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4878"},"modified":"2026-09-15T12:03:41","modified_gmt":"2026-09-15T04:03:41","slug":"oligo-dt25-affinitatschromatographieharz","status":"publish","type":"page","link":"https:\/\/biovanix.com\/de\/oligo-dt25-affinity-chromatography-resin\/","title":{"rendered":"Oligo dT(25) Affinit\u00e4tschromatographie-Harz"},"content":{"rendered":"
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Oligo dT(25) Affinit\u00e4tschromatographie-Harz<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Biovanix Oligo dT(25) Affinit\u00e4tsharz<\/strong>\u00a0ist eine hochmoderne L\u00f6sung f\u00fcr die Reinigung und Isolierung von mRNA aus In-vitro-Transkriptionsverfahren (IVT). Dieses fortschrittliche Harz f\u00e4ngt die mRNA selektiv \u00fcber ihren polyadenylierten (polyA) Schwanz ab und trennt sie effektiv von anderen Komponenten der Transkriptionsreaktion, wie Enzymen und Plasmid-DNA.<\/p>

Perfekt geeignet f\u00fcr die nachgeschaltete Aufreinigung in gro\u00dfem Ma\u00dfstab,\u00a0Biovanix Oligo dT(25) Affinit\u00e4tsharz<\/strong>\u00a0ist ein Schl\u00fcsselfaktor f\u00fcr Impfstoff- und Gentherapieanwendungen und bietet eine un\u00fcbertroffene Selektivit\u00e4t und Kapazit\u00e4t, um die Anforderungen der modernen biopharmazeutischen Herstellung zu erf\u00fcllen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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50 \u03bcm<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Partikelgr\u00f6\u00dfe<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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200 nm<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Porengr\u00f6\u00dfe<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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pH 2\u201313<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Stability Range<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Maximaler Druck<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Stability Range<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Produktmerkmale<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Engineered for High-Performance mRNA Purification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Biovanix Oligo dT(25) Affinity Resin combines selective polyA capture with a rigid PS-DVB matrix for reliable results at any scale.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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1. Selektiver mRNA-Einfang<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 PolyA Tail Specificity:<\/strong>\u00a0Zielt mit hoher Selektivit\u00e4t auf mRNA und gew\u00e4hrleistet eine effektive Entfernung von Plasmid-DNA und anderen IVT-Komponenten.<\/p>

\u2714Simple Salt and Water Elution:<\/strong>\u00a0Rationalisierter Reinigungsprozess mit minimaler Komplexit\u00e4t.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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2. Skalierbare L\u00f6sung<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Ausgezeichnete Skalierbarkeit:<\/strong>\u00a0Konzipiert f\u00fcr den nahtlosen \u00dcbergang vom Laborma\u00dfstab zur Produktion im gro\u00dfen Ma\u00dfstab.<\/p>

\u2714 High Capacity: <\/strong>Erf\u00fcllt die Anforderungen der mRNA-Herstellung mit hohem Ertrag.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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3. Nicht aus Tieren gewonnen<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Synthetischer Herstellungsprozess:<\/strong>\u00a0Die Oligoliganden von Biovanix sind frei von tierischen Bestandteilen, wodurch die Einhaltung gesetzlicher Vorschriften und ethischer Standards gew\u00e4hrleistet ist.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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4. Vereinfachter Arbeitsablauf<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Geringere Komplexit\u00e4t:<\/strong>\u00a0Macht mehrere Reinigungsschritte \u00fcberfl\u00fcssig, maximiert die Effizienz und reduziert die Komplexit\u00e4t der nachgeschalteten Prozesse.<\/p>

\u2714 <\/strong>Konsistente Leistung:<\/strong>\u00a0Verl\u00e4ssliche Ergebnisse \u00fcber verschiedene Ma\u00dfst\u00e4be und Produktionschargen hinweg.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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How It Works<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Simple 4-Step mRNA Purification Workflow<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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From crude IVT mixture to purified mRNA in a single affinity capture step \u2014 no complex multi-step protocols required.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2460<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Equilibrate<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Prepare column with high-salt binding buffer (e.g., 0.5 M NaCl)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2461<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Load & Bind<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Apply IVT mixture; polyA-tailed mRNA binds to poly(dT) ligands<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2462<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Wash<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Remove impurities (enzymes, pDNA, nucleotides) in flow-through<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2463<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Elute<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Release purified mRNA with water or low-salt buffer<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Technical Specifications<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Product Specifications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Comprehensive technical data for method development and process validation.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Charakteristisch<\/strong><\/td>Beschreibung<\/strong><\/td><\/tr>
Support-Matrix<\/strong><\/td>Vernetztes Poly(styrol-Divinylbenzol)<\/td><\/tr>
Durchschnittliche Partikelgr\u00f6\u00dfe<\/strong><\/td>50 um<\/td><\/tr>
Durchschnittliche Porengr\u00f6\u00dfe<\/strong><\/td>200 nm<\/td><\/tr>
Oberfl\u00e4chenfunktionalit\u00e4t<\/strong><\/td>Poly(dT) 25mer mit propriet\u00e4rem Linker<\/td><\/tr>
Ligandenkonzentration<\/strong><\/td>0,3 umol\/ml<\/td><\/tr>
Mechanische Best\u00e4ndigkeit<\/strong><\/td>70 bar (1.000 psi; 7 MPa)<\/td><\/tr>
Thermische Stabilit\u00e4t<\/strong><\/td>erm\u00f6glicht bei Bedarf die Denaturierung der Probe bei 65\u00b0C<\/td><\/tr>
pH-Bereich<\/strong><\/td>2-13<\/td><\/tr>
Bereich der Ionenst\u00e4rke<\/strong><\/td>0 bis 5 M, alle g\u00e4ngigen Salze<\/td><\/tr>
Chemische Best\u00e4ndigkeit<\/strong><\/td>\u00dcbliche Mittel f\u00fcr die mRNA-Reinigung sind 0,5 M NaOH, 2 M MgCl2, 20 mM EDTA. Wasser, 0 bis 100% Alkohol, Acetonitril, 2 M Essigs\u00e4ure, 1 M HCl und andere g\u00e4ngige organische L\u00f6sungsmittel<\/td><\/tr>
Lagerung<\/strong><\/td>18-20% Ethanol<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Chemische und thermische Best\u00e4ndigkeit<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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pH-Bereich<\/strong><\/td>2-13<\/td><\/tr>
Bereich der Ionenst\u00e4rke<\/strong><\/td>0 bis 5 M, alle g\u00e4ngigen Salze<\/td><\/tr>
Puffer Zusatzstoffe<\/strong><\/td>

\u00dcbliche Mittel f\u00fcr die mRNA-Reinigung sind 0,5 M NaOH, 2 MMgCl2, 20 mM EDTA.<\/p>

Nicht mit starken Oxidationsmitteln (wie Hypochlorit), oxidierenden S\u00e4uren (wie Salpeters\u00e4ure), starken Reduktionsmitteln (wie Sulfit), Aceton, THF oder Benzylalkohol in Ber\u00fchrung bringen.<\/p><\/td><\/tr>

L\u00f6sungsmittel<\/strong><\/td>

Wasser, 0 bis 100% Alkohol, Acetonitril, 2 M Essigs\u00e4ure, 1 M HCl und andere \u00fcbliche organische L\u00f6sungsmittel.<\/p>

Nicht mit starken Oxidationsmitteln (wie Hypochlorit), oxidierenden S\u00e4uren (wie Salpeters\u00e4ure), starken Reduktionsmitteln (wie Sulfit), Aceton, Benzylalkohol oder THF in Ber\u00fchrung bringen.<\/p><\/td><\/tr>

Durchflussmenge<\/strong><\/td>Durchflussmenge je nach Leistung anpassen. Obere Druckgrenzen nicht \u00fcberschreiten.<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Vorteile auf einen Blick<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Why Biovanix Oligo dT(25) Resin<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Designed to maximize purity, simplify workflows, and reduce total cost of ownership.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u26a1<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Hoher Wirkungsgrad<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Schnelle und effiziente mRNA-Aufreinigung.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udcc8<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Skalierbarer Prozess<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Leicht anpassbar an die Gro\u00dfserienfertigung.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udee1\ufe0f<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Einhaltung von Vorschriften<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Synthetisches Herstellungsverfahren ohne Tierversuche.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udcb0<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Kosteng\u00fcnstig<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Vereinfachte Arbeitsabl\u00e4ufe reduzieren die betriebliche Komplexit\u00e4t und die Kosten.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Anwendungen<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Built for the mRNA Revolution<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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From vaccine development to gene therapy manufacturing, Biovanix Oligo dT(25) Resin supports the full spectrum of mRNA applications.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Impfstoffproduktion<\/h3>

Effiziente Reinigung von mRNA-Impfstoffen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Gentherapie<\/h3>

Skalierbare Produktion von mRNA f\u00fcr therapeutische Anwendungen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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IVT Fertigung<\/h3>

Rationalisierte Isolierung von mRNA aus Transkriptionsreaktionsmischungen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Entfernung von Plasmid-DNA<\/h3>

Wirksame Beseitigung von Plasmid-DNA und anderen IVT-Bestandteilen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Why Choose Biovanix<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Your Trusted Partner in mRNA Purification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u2460\u00a0Efficient mRNA Purification<\/h3>

Erzielen Sie hochreine mRNA mit minimalen Verunreinigungen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2461\u00a0Cost-Effective<\/h3>

Geringere betriebliche Komplexit\u00e4t und h\u00f6here Produktivit\u00e4t senken die Gesamtproduktionskosten.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2462 Regulatory Compliance<\/h3>

Die tierversuchsfreie und synthetische Herstellung gew\u00e4hrleistet die Einhaltung globaler Standards.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2463\u00a0Versatile Application<\/h3>

Ideal f\u00fcr eine breite Palette von mRNA-Konstrukten, die in Impfstoffen, Gentherapien und anderen biopharmazeutischen Produkten verwendet werden.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Frequently Asked Questions<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Oligo dT(25) Resin FAQ<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Common questions about mRNA purification with Oligo dT(25) affinity chromatography.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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What is Oligo dT(25) affinity chromatography resin and how does it work? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Oligo dT(25) affinity chromatography resin is a specialized chromatography medium functionalized with poly(deoxythymidine) 25-mer ligands attached to a solid support matrix. It works through complementary A-T base pairing: the poly(dT) ligands on the resin selectively bind to the polyadenylated (polyA) tail of mRNA molecules. When an IVT (in vitro transcription) mixture is loaded onto the column under high-salt conditions, mRNA is captured while impurities such as enzymes, plasmid DNA, and unincorporated nucleotides pass through. Elution is achieved with water or low-salt buffer, releasing the purified mRNA.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the principle behind mRNA purification using oligo dT affinity chromatography? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The purification principle relies on specific Watson-Crick A-T base pairing between the poly(dT) 25-mer ligand immobilized on the resin and the polyA tail present on mature mRNA. Under high ionic strength conditions (typically 0.5 M NaCl), electrostatic shielding stabilizes the hybridization between poly(dT) and polyA. The mRNA binds selectively to the resin while process impurities flow through. Elution is performed under low-ionic-strength conditions (water or dilute buffer), which destabilizes the hybrid and releases purified mRNA in a single chromatographic step.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What types of mRNA can be purified with Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Oligo dT(25) resin can purify any polyadenylated mRNA produced through in vitro transcription (IVT), including mRNA constructs for vaccines (such as infectious disease vaccines), gene therapy mRNAs (such as those encoding therapeutic proteins or gene editing components), and research-grade mRNAs of various lengths. The resin is compatible with mRNA constructs ranging from short transcripts to several thousand nucleotides in length, as long as they contain a polyA tail.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the binding capacity of Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The binding capacity of Oligo dT(25) resin depends on the mRNA construct length, loading conditions, and the specific product formulation. Generally, affinity resins with poly(dT) ligands offer high dynamic binding capacity suitable for preparative-scale mRNA purification. Smaller mRNA constructs tend to achieve higher binding capacity per mL of resin due to reduced steric hindrance. For specific capacity data for your mRNA construct, contact the Biovanix technical team for application-specific recommendations.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What are the advantages of PS-DVB matrix vs. agarose for mRNA purification? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Poly(styrene-divinylbenzene) (PS-DVB) matrices offer several advantages over traditional agarose gels for mRNA purification: (1) Higher mechanical strength \u2014 PS-DVB beads are rigid, enabling higher flow rates and pressure tolerance (up to 70 bar for Biovanix resin) without bed compression; (2) Broader chemical compatibility \u2014 stable across pH 2\u201313 and resistant to organic solvents, enabling aggressive cleaning-in-place (CIP) with NaOH; (3) Longer resin lifetime \u2014 the rigid polymer backbone does not compress or degrade over repeated cycles; (4) Better scalability \u2014 linear pressure-flow behavior allows consistent performance from lab to production scale; (5) No risk of gel compression artifacts during scale-up.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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How do I pack and use Oligo dT(25) resin in my chromatography system? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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General workflow: (1) Equilibrate the column with binding buffer (high-salt buffer, e.g., 0.5 M NaCl) to prepare the resin for mRNA capture; (2) Load the IVT reaction mixture onto the column \u2014 the mRNA polyA tail binds to the poly(dT) ligands while impurities flow through; (3) Wash the column with binding buffer or a moderate-salt wash buffer to remove residual impurities; (4) Elute purified mRNA with water or low-salt buffer (e.g., 10 mM Tris-HCl); (5) Clean and regenerate the column with appropriate CIP solution (e.g., 0.1\u20130.5 M NaOH) for reuse. Always adjust flow rate to stay within the pressure limit (70 bar maximum for Biovanix resin). For denaturing secondary structures, samples can be heated to 65\u00b0C during processing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What flow rate and pressure should I use with Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Biovanix Oligo dT(25) resin has a maximum mechanical resistance of 70 bar (1,000 psi \/ 7 MPa). The flow rate should be adjusted based on your specific application and column dimensions, ensuring that backpressure does not exceed this limit. The 50 \u03bcm average particle size allows efficient mass transfer at moderate to high linear flow rates. Start at a conservative flow rate during method development and optimize based on binding efficiency and recovery. The rigid PS-DVB matrix maintains stable bed volume across flow rate changes, providing consistent performance.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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Can I reuse the Oligo dT(25) resin and how do I clean it? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Yes, Oligo dT(25) resin can be reused for multiple purification cycles with proper cleaning and storage. Cleaning-in-place (CIP) is typically performed using 0.1\u20130.5 M NaOH, which effectively removes residual nucleic acids and contaminants. The resin’s broad pH stability (2\u201313) and chemical resistance support stringent cleaning protocols. After CIP, re-equilibrate the column with binding buffer before the next use. Store the resin in 18\u201320% ethanol at 2\u20138\u00b0C when not in use. Do not freeze. Resin lifetime depends on the application, cleaning protocol, and feed material quality.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What buffer conditions are recommended for mRNA binding and elution? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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For mRNA binding (capture): use a high-salt buffer such as 0.5 M NaCl with an appropriate buffer component (e.g., 10 mM Tris-HCl, pH 7.5). The high ionic strength provides electrostatic shielding that stabilizes the A-T hybridization between poly(dT) ligands and the mRNA polyA tail. For washing: use the same high-salt binding buffer or a moderately reduced salt concentration. For elution: use deionized water or a low-salt buffer (e.g., 10 mM Tris-HCl without NaCl) to destabilize the hybrid and release purified mRNA. The resin also allows sample denaturing at 65\u00b0C if needed to eliminate undesired higher-order RNA structures.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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How does Oligo dT(25) resin remove plasmid DNA and other IVT impurities? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The selectivity of Oligo dT(25) resin is based on the specific A-T base pairing between the poly(dT) ligands and the polyA tail of mRNA. Only polyadenylated mRNA molecules bind to the resin. Plasmid DNA (which lacks a polyA tail), RNA polymerase enzymes, unincorporated nucleotides, short abortive transcripts, and buffer components do not bind and are removed in the flow-through and wash fractions. This single-step affinity capture effectively separates full-length mRNA from the major IVT process impurities, significantly reducing downstream processing complexity.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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Can Oligo dT(25) resin be used for continuous or automated mRNA purification? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Yes, Oligo dT(25) resin is compatible with standard chromatography systems and can be adapted for automated and continuous processing workflows. The rigid PS-DVB matrix provides consistent pressure-flow behavior, making it suitable for use on automated chromatography platforms. The simple salt-and-water elution workflow minimizes buffer exchange requirements. For process development and screening, smaller column formats can be used to optimize conditions before scaling to larger automated systems. The linear scalability of the PS-DVB matrix ensures that conditions optimized at small scale translate directly to production scale.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the shelf life and storage condition for Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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