{"id":3797,"date":"2025-01-20T14:15:33","date_gmt":"2025-01-20T14:15:33","guid":{"rendered":"https:\/\/biovanix.com\/?p=3797"},"modified":"2025-01-20T14:26:36","modified_gmt":"2025-01-20T14:26:36","slug":"wie-wahlt-man-die-richtige-siliziumdioxid-hplc-saule-aus","status":"publish","type":"post","link":"https:\/\/biovanix.com\/de\/wie-wahlt-man-die-richtige-siliziumdioxid-hplc-saule-aus\/","title":{"rendered":"Wie w\u00e4hlt man die richtige HPLC-S\u00e4ule mit Silika aus?"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"3797\" class=\"elementor elementor-3797\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-19664e9 e-flex e-con-boxed e-con e-parent\" data-id=\"19664e9\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2aeab5f elementor-widget elementor-widget-text-editor\" data-id=\"2aeab5f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1><span style=\"color: #33615a;\">Wie w\u00e4hlt man die richtige Silika-HPLC-S\u00e4ule aus?<\/span><\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-340a0eb e-flex e-con-boxed e-con e-parent\" data-id=\"340a0eb\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-39437b4 elementor-widget elementor-widget-text-editor\" data-id=\"39437b4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-col-group fl-node-5e1d10f001116\" data-node=\"5e1d10f001116\"><div class=\"fl-col fl-node-5e1d10f0011e3 fl-col-bg-color fl-col-has-cols\" data-node=\"5e1d10f0011e3\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-rich-text fl-node-5e1d10eff3aac\" data-node=\"5e1d10eff3aac\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h2>Die Auswahl von Kieselgel-HPLC-S\u00e4ulen<\/h2><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-5e1d113847353\" data-node=\"5e1d113847353\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><p>Trotz seiner Porosit\u00e4t weist kugelf\u00f6rmiges por\u00f6ses HPLC-Siliciumdioxid im Vergleich zu anderen Materialien eine hohe mechanische Festigkeit auf. Au\u00dferdem l\u00e4sst es sich leicht chemisch modifizieren. F\u00fcr die Normalphasen-HPLC steht eine breite Palette por\u00f6ser Kiesels\u00e4uren zur Verf\u00fcgung, die durch Messungen der Oberfl\u00e4che, der Porengr\u00f6\u00dfe und der Partikelgr\u00f6\u00dfe gekennzeichnet sind. Die Verwendung der Normalphasen-HPLC wird nicht durch die Probleme der Aufl\u00f6sung von Silika oder des Peak-Tailing eingeschr\u00e4nkt, die bei der Umkehrphasen-HPLC auftreten.<\/p><p><strong>1. Partikelgr\u00f6\u00dfe<\/strong><\/p><p><strong>2. Porengr\u00f6\u00dfe<\/strong><\/p><p><strong>3. Andere physische Merkmale<\/strong><\/p><h2>Partikelgr\u00f6\u00dfe<\/h2><p>Da sich die Qualit\u00e4t und Reproduzierbarkeit von por\u00f6sem Siliziumdioxid verbessert, werden f\u00fcr analytische Arbeiten zunehmend Materialien mit einer Partikelgr\u00f6\u00dfe von 3, 5 oder 10 \u03bcm verwendet. 10-\u03bcm-Partikel werden weniger h\u00e4ufig verwendet, sind aber nach wie vor eine wichtige Partikelgr\u00f6\u00dfe f\u00fcr pr\u00e4parative Anwendungen. Aus wirtschaftlichen Gr\u00fcnden werden unregelm\u00e4\u00dfige Kiesels\u00e4uren nach wie vor h\u00e4ufig in der industriellen Reinigung eingesetzt.<\/p><h2><strong>Porengr\u00f6\u00dfe<\/strong><\/h2><p>Die \u00fcbliche Porengr\u00f6\u00dfe f\u00fcr por\u00f6se Kieselerde ist 100A, 120A, 300A. Einige Anbieter bieten auch Kiesels\u00e4ure mit 80A, 200A oder sogar 450A an. Die Auswahl der Porengr\u00f6\u00dfe richtet sich nach der Gr\u00f6\u00dfe der Zielproben. F\u00fcr die herk\u00f6mmliche Analyse kleiner Molek\u00fcle wird die Polgr\u00f6\u00dfe 100A\/120A verwendet. F\u00fcr die Analyse gro\u00dfer Molek\u00fcle werden 300A verwendet. Bei bestimmten Proben richtet sich die Wahl der Porengr\u00f6\u00dfe im Siliziumdioxid nach der Molek\u00fclgr\u00f6\u00dfe.<\/p><h2>Andere physische Merkmale<\/h2><p>Die physikalischen Eigenschaften der neueren Kiesels\u00e4urepartikel wurden in mehrfacher Hinsicht verbessert. Viele von ihnen sind jedoch nicht ohne weiteres erh\u00e4ltlich, da sie haupts\u00e4chlich als Ausgangsmaterial f\u00fcr die Herstellung neuer Umkehrphasen-Kiesels\u00e4uren verwendet werden.<\/p><ul><li>Oberfl\u00e4chenaktivit\u00e4t<\/li><\/ul><p>Es wird ein geringerer Anteil an unerw\u00fcnschten, freien und isolierten Silanolgruppen beobachtet. Der geringere Gehalt an Metallionenverunreinigungen tr\u00e4gt teilweise zu diesem R\u00fcckgang der Oberfl\u00e4chenaktivit\u00e4t bei. Basische Verbindungen interagieren weniger stark mit der Silika-Oberfl\u00e4che, was zu einer verbesserten Chromatographie f\u00fchrt.<\/p><ul><li>Physikalische Eigenschaften<\/li><\/ul><p>Durch die verbesserte Kontrolle der physikalischen Eigenschaften wie Oberfl\u00e4che, Porenvolumen, mittlerer Porendurchmesser und Partikelgr\u00f6\u00dfe sind die neuen Kiesels\u00e4uren von Charge zu Charge besser reproduzierbar.<\/p><ul><li>Reinheit<\/li><\/ul><p>Der Gehalt an Metallionenverunreinigungen konnte in einigen F\u00e4llen auf kumulative Werte &lt; 10 ppm gesenkt werden. Die unerw\u00fcnschte Chelatbildung von Metallionen und gel\u00f6sten Stoffen wurde minimiert.<\/p><\/div><\/div><\/div><\/div><\/div><div class=\"fl-col fl-node-60dab52fd22c6 fl-col-bg-color fl-col-small fl-col-has-cols\" data-node=\"60dab52fd22c6\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-col-group fl-node-60dab53776a78 fl-col-group-nested\" data-node=\"60dab53776a78\"><div class=\"fl-col fl-node-5e1d11255fb0b fl-col-bg-color\" data-node=\"5e1d11255fb0b\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-sidebar fl-node-5e1d11255fa16\" data-node=\"5e1d11255fa16\"><div class=\"fl-module-content fl-node-content\"><aside id=\"block-26\" class=\"widget widget_block widget_search\"><form class=\"wp-block-search__no-button wp-block-search\" role=\"search\" action=\"https:\/\/galaklc.com\/\" method=\"get\"><label class=\"wp-block-search__label screen-reader-text\" for=\"wp-block-search__input-1\">Suche<\/label><\/form><\/aside><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-842b315 e-flex e-con-boxed e-con e-parent\" data-id=\"842b315\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f00362d elementor-widget elementor-widget-text-editor\" data-id=\"f00362d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Biovanix Kiesels\u00e4ure-Packungsmaterial<\/h2><table><tbody><tr><td width=\"151\"><strong>Partikelgr\u00f6\u00dfe<\/strong><\/td><td width=\"115\"><strong>Porengr\u00f6\u00dfe<\/strong><\/td><td width=\"145\"><strong>Fl\u00e4che<\/strong><\/td><td width=\"130\"><strong>pH-Bereich<\/strong><\/td><\/tr><tr><td width=\"151\">3\/5\/10\/15\/20\/ 50\u03bcm<\/td><td width=\"115\">100\u00c5, 300\u00c5<\/td><td width=\"145\">330m<sup>2<\/sup>\/g, 200m<sup>2<\/sup>\/g<\/td><td width=\"130\">2-8<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-76387c9d e-flex e-con-boxed e-con e-parent\" data-id=\"76387c9d\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-471ea402 e-con-full e-flex e-con e-child\" data-id=\"471ea402\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1abb2595 elementor-widget elementor-widget-text-editor\" data-id=\"1abb2595\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">Haben Sie Probleme mit der Fl\u00fcssigkeitschromatographie? Kontaktieren Sie uns direkt.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-10e77a30 e-con-full e-flex e-con e-child\" data-id=\"10e77a30\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-31e46092 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"31e46092\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Kontakt<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>How to choose proper silica HPLC column? The selection of silica HPLC columns Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"left-sidebar","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1,49],"tags":[],"class_list":["post-3797","post","type-post","status-publish","format-standard","hentry","category-blog","category-hplc-introduction"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/de\/author\/biovanix-com\/"},"uagb_comment_info":28,"uagb_excerpt":"How to choose proper silica HPLC column? The selection of silica HPLC columns Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"How to choose proper silica HPLC column? The selection of silica HPLC columnsDespite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size measurements. 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The selection of silica HPLC columnsDespite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size measurements. 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