{"id":5133,"date":"2025-02-27T14:38:12","date_gmt":"2025-02-27T14:38:12","guid":{"rendered":"https:\/\/biovanix.com\/?p=5133"},"modified":"2025-02-27T15:00:52","modified_gmt":"2025-02-27T15:00:52","slug":"einstufige-reinigung-fur-lebenden-attenuierten-tollwutvirusimpfstoff","status":"publish","type":"post","link":"https:\/\/biovanix.com\/de\/einstufige-reinigung-fur-lebenden-attenuierten-tollwutvirusimpfstoff\/","title":{"rendered":"Ein-Schritt-Reinigung f\u00fcr lebenden abgeschw\u00e4chten Tollwutvirus-Impfstoff"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"5133\" class=\"elementor elementor-5133\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c72fc52 e-flex e-con-boxed e-con e-parent\" data-id=\"c72fc52\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b0a4716 elementor-widget elementor-widget-heading\" data-id=\"b0a4716\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Ein-Schritt-Reinigung f\u00fcr lebenden abgeschw\u00e4chten Tollwutvirus-Impfstoff<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-02ded2e elementor-widget elementor-widget-text-editor\" data-id=\"02ded2e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Tollwut ist eine vermeidbare Viruserkrankung, die meist durch den Biss eines tollw\u00fctigen Tieres \u00fcbertragen wird. Das Tollwutvirus infiziert das zentrale Nervensystem von S\u00e4ugetieren und verursacht schlie\u00dflich eine Erkrankung des Gehirns und den Tod (<a href=\"https:\/\/www.cdc.gov\/rabies\/about.html\">CDC<\/a>). In bis zu 99% der F\u00e4lle sind Haushunde f\u00fcr die \u00dcbertragung des Tollwutvirus auf den Menschen verantwortlich. Tollwut kann jedoch sowohl Haus- als auch Wildtiere befallen. Auf den Menschen wird sie durch Bisse oder Kratzer \u00fcbertragen, in der Regel \u00fcber den Speichel. Ohne Tollwutimpfung liegt die Sterblichkeitsrate bei fast 100%, wenn Menschen und Haustiere infiziert sind. (<a href=\"https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/rabies\">Weltgesundheitsorganisation<\/a>). Tollwutimpfstoffe sind die wirksamste Methode, um Menschen und Haustiere zu sch\u00fctzen, wenn sie der Krankheit ausgesetzt sind.<\/p><p>Im Vergleich zur herk\u00f6mmlichen Reinigung von Tollwutimpfstoffen (Membrantrennung und Gr\u00f6\u00dfenausschluss) k\u00f6nnen die Biovanix Biopure AF-Medien das abgeschw\u00e4chte Tollwut-Lebendvirus in nur einem Schritt mit einer besseren Wiedergewinnung und Reinheit des Virusproteins reinigen.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7a4790f e-flex e-con-boxed e-con e-parent\" data-id=\"7a4790f\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dd7ccf0 elementor-widget elementor-widget-text-editor\" data-id=\"dd7ccf0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Tollwut-Impfstoff-Reinigungstests<\/h2><h3>Ergebnis<\/h3><ul><li>Wiederherstellung &gt; 80%<\/li><li>Reinheit &gt; 90%<\/li><li>Beseitigungsrate von Verunreinigungen &gt; 99%<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-94a9f8d e-flex e-con-boxed e-con e-parent\" data-id=\"94a9f8d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-c71e931 e-con-full e-flex e-con e-child\" data-id=\"c71e931\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e52b74a elementor-widget elementor-widget-text-editor\" data-id=\"e52b74a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Testbedingungen:<\/b><\/p><ul><li>S\u00e4ule:  VirCap\u00ae AF 1ml vorgepackte S\u00e4ule<\/li><li>Mobile Phase A: 10mM PB pH7,4<\/li><li>Mobile Phase B: 10mM PB pH7,4 2M NaCl<\/li><li>Durchflussmenge: 0,5ml\/min<\/li><li>Pr\u00fcfung: UV 280nm<\/li><li>Probe: Gekl\u00e4rtes Virusmedium<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-f851655 e-con-full e-flex e-con e-child\" data-id=\"f851655\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a8953a8 elementor-widget elementor-widget-image\" data-id=\"a8953a8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"174\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-300x174.jpg\" class=\"attachment-medium size-medium wp-image-5118\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-300x174.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-768x444.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-600x347.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2a32318 e-flex e-con-boxed e-con e-parent\" data-id=\"2a32318\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-95f020d elementor-widget elementor-widget-text-editor\" data-id=\"95f020d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Verwendung der HPSEC-Analyse f\u00fcr die Tests von Probenl\u00f6sung, Perkolationsfl\u00fcssigkeit und Sammelfl\u00fcssigkeit, und kein Peak des Targets im Durchbruch bei RT=10min. Biopure AF-Medien eluieren Viruspartikel durch Ver\u00e4nderung der Ionenst\u00e4rke in der mobilen Phase. Sowohl die Reinheit als auch die Konzentration des Virus verbessern sich deutlich.<\/p><p>Parallele Tests der HPSEC-Analyse f\u00fcr die Erfassung von Zielspitzen werden auch unter den Wettbewerbern durchgef\u00fchrt.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-37c9f2e e-flex e-con-boxed e-con e-parent\" data-id=\"37c9f2e\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-0918eb5 e-con-full e-flex e-con e-child\" data-id=\"0918eb5\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cda7b79 elementor-widget elementor-widget-image\" data-id=\"cda7b79\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"169\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-169x300.jpg\" class=\"attachment-medium size-medium wp-image-5134\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-169x300.jpg 169w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification.jpg 338w\" sizes=\"(max-width: 169px) 100vw, 169px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-db14fdc e-con-full e-flex e-con e-child\" data-id=\"db14fdc\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-94d95b0 elementor-widget elementor-widget-image\" data-id=\"94d95b0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"181\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-300x181.jpg\" class=\"attachment-medium size-medium wp-image-5135\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-300x181.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-768x463.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-600x362.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a85ce24 e-flex e-con-boxed e-con e-parent\" data-id=\"a85ce24\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d57dad7 elementor-widget elementor-widget-text-editor\" data-id=\"d57dad7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-module fl-module-rich-text fl-node-5e68a113ddd36\" data-node=\"5e68a113ddd36\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h2><b>KVP und Freigabe<\/b><\/h2><p>Die H\u00e4ufigkeit des Clean-in-Place (CIP) h\u00e4ngt von den Eigenschaften und Bedingungen des L\u00f6sungsmittels ab.<\/p><p>F\u00fcr den Abscheidungsschritt wird nach jedem Durchlauf ein mindestens 30-min\u00fctiges CIP-Verfahren mit 1 M NaOH im Gegenstrom angenommen.<\/p><p>Zur Verringerung der mikrobiellen Kontamination in der vorgepackten S\u00e4ule wird eine Reinigung mit 0,5 bis 1,0 M NaOH mit einer Kontaktzeit von 1 h empfohlen.<\/p><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-609b908faae17\" data-node=\"609b908faae17\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h3>Eine weitere Methode zur Reinigung des Tollwutvirus<\/h3><p><a href=\"http:\/\/galaklc.com\/chromatography-purification-of-inactive-rabies-virus\/\">Chromatographische Reinigung des inaktiven Tollwutvirus<\/a><\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). In up to 99% of cases, domestic dogs are responsible for rabies [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-5133","post","type-post","status-publish","format-standard","hentry","category-blog"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/de\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). In up to 99% of cases, domestic dogs are responsible for rabies&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). 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