{"id":2186,"date":"2024-05-17T03:27:26","date_gmt":"2024-05-17T03:27:26","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=2186"},"modified":"2025-03-04T15:18:20","modified_gmt":"2025-03-04T15:18:20","slug":"protein-a-affinity-hplc-column-for-mab-analysis","status":"publish","type":"page","link":"https:\/\/biovanix.com\/en\/protein-a-affinity-hplc-column-for-mab-analysis\/","title":{"rendered":"Protein A Affinity HPLC Column For mAb Analysis"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2186\" class=\"elementor elementor-2186\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3e866b3 e-flex e-con-boxed e-con e-parent\" data-id=\"3e866b3\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-53c97f5 e-con-full e-flex e-con e-child\" data-id=\"53c97f5\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-993e5dd elementor-widget elementor-widget-image\" data-id=\"993e5dd\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2190\" alt=\"Biovanix Protein A HPLC Column\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-100x100.jpg 100w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column.jpg 500w\" sizes=\"(max-width: 300px) 100vw, 300px\" title=\"Biovanix Protein A HPLC Column | BioVanix\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d6e4b77 e-con-full e-flex e-con e-child\" data-id=\"d6e4b77\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e959cf2 elementor-widget elementor-widget-heading\" data-id=\"e959cf2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Prosep Protein A Affinity HPLC Column<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b4ed5e1 elementor-widget elementor-widget-heading\" data-id=\"b4ed5e1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">For mAb HPLC Analysis<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-61a1c91 elementor-widget elementor-widget-text-editor\" data-id=\"61a1c91\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions.<\/p>\n<ul>\n<li>Direct use on HPLC instrument<\/li>\n<li>Fast and accurate analysis of antibody concentration<\/li>\n<li>Wide linearity range<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1000114 elementor-widget elementor-widget-text-editor\" data-id=\"1000114\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #ffffff;\"><a style=\"color: #ffffff;\" title=\"Comparison Test Report For Prosep Protein A Affinity HPLC Column\" href=\"https:\/\/biovanix.com\/comparison-test-report-for-prosep-protein-a-affinity-hplc-column\/\"><span style=\"text-decoration: underline;\">Comparison test report with POR*S A column in China TOP CXO manufacturer, click here &gt;&gt;&gt;<\/span><\/a><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0086744 e-flex e-con-boxed e-con e-parent\" data-id=\"0086744\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c3dccd6 elementor-widget elementor-widget-text-editor\" data-id=\"c3dccd6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><strong>Technical Parameter<\/strong><\/h2>\n<table>\n<tbody>\n<tr>\n<td>\n<p><strong>\u00a0<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Ligand<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Substrate<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Pressure<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>pH Range<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Dynamic Capacity<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Flow Rate<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Life Time<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p>Prosep Protein A PLUS Column<\/p>\n<\/td>\n<td>\n<p>rProtein A<\/p>\n<\/td>\n<td>\n<p>20 \u03bcm PSDVB<\/p>\n<\/td>\n<td>\n<p>1200psi<\/p>\n<\/td>\n<td>\n<p>2-11<\/p>\n<\/td>\n<td>\n<p>40mg human IgG\/ml media[1]<\/p>\n<\/td>\n<td>\n<p>1-3ml\/min<\/p>\n<\/td>\n<td>\n<p>1000-3000 circles<span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-weight: var( --e-global-typography-text-font-weight ); text-align: var(--text-align); background-color: var(--ast-global-color-5); font-size: 1rem;\">[2]<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p>Prosep Protein A Column<\/p>\n<\/td>\n<td>\n<p>rProtein A<\/p>\n<\/td>\n<td>\n<p>40 \u03bcm PSDVB<\/p>\n<\/td>\n<td>\n<p>1200psi<\/p>\n<\/td>\n<td>\n<p>2-11<\/p>\n<\/td>\n<td>\n<p>40mg human IgG\/ml media<span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-weight: var( --e-global-typography-text-font-weight ); text-align: var(--text-align); background-color: var(--ast-global-color-5); font-size: 1rem;\">[1]<\/span><\/p>\n<\/td>\n<td>\n<p>1-3ml\/min<\/p>\n<\/td>\n<td>\n<p>1000-3000 circles[2]<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ol>\n<li>PS\/DVB: Poly(styrene\/divinylbenzene)\u00a0<\/li>\n<li>The testing result is based on local laboratory tests.<\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-39d9182 e-flex e-con-boxed e-con e-parent\" data-id=\"39d9182\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-42979f0 e-con-full e-flex e-con e-child\" data-id=\"42979f0\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ed4ce2c elementor-widget elementor-widget-text-editor\" data-id=\"ed4ce2c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Prosep Protein A Product<\/h2>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-36f357d e-con-full e-flex e-con e-child\" data-id=\"36f357d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c395611 elementor-widget elementor-widget-image\" data-id=\"c395611\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/en\/product\/protein-a-affinity-hplc-column\/\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2190\" alt=\"Biovanix Protein A HPLC Column\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-100x100.jpg 100w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column.jpg 500w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-734e1a86 e-flex e-con-boxed e-con e-parent\" data-id=\"734e1a86\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-46c36a35 elementor-widget elementor-widget-text-editor\" data-id=\"46c36a35\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\n<h2><strong>Analysis Condition For Prosep Protein A Column<\/strong><\/h2>\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\"><strong>Eluent\u00a0A<\/strong><\/th>\n<th align=\"left\"><strong>20-50\u00a0mM\u00a0Phosphate,\u00a0150\u00a0mM\u00a0NaCl,\u00a0pH7-7.5<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\"><strong>Eluent\u00a0B<\/strong><\/td>\n<td align=\"left\">12 mM HCl, 150 mM NaCl, pH1.9,\u00a0or 100 mM Gly-HCl, 150 mM NaCl, pH2.5<\/td>\n<\/tr>\n<tr>\n<th align=\"left\"><strong>Flow\u00a0rate<\/strong><\/th>\n<th align=\"left\"><strong>1\u00a0mL\/min<\/strong><\/th>\n<\/tr>\n<tr>\n<td align=\"left\"><strong>Injection<\/strong><\/td>\n<td align=\"left\">1-100\u00a0\u03bcL<\/td>\n<\/tr>\n<tr>\n<th align=\"left\"><strong>Detection<\/strong><\/th>\n<th align=\"left\">UV\u00a0280nm<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>Method Timetable<\/strong><\/p>\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\"><strong>Time(min)<\/strong><\/th>\n<th align=\"left\"><strong>Gradient(%B)<\/strong><\/th>\n<th align=\"left\">\u00a0<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">0.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">Equilibration\/Wash, 1 min, 10 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">1.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">Equilibration\/Wash, 1 min, 10 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">1.01<\/td>\n<td align=\"left\">100<\/td>\n<td align=\"left\">Elution, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">3.00<\/td>\n<td align=\"left\">100<\/td>\n<td align=\"left\">Elution, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">3.01<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">Reequilibration, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">5.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">Reequilibration, 2 min, 20 CV<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>* The analysis condition varies according to different machines.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-71a0e466 e-flex e-con-boxed e-con e-parent\" data-id=\"71a0e466\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-650a23d1 e-con-full e-flex e-con e-child\" data-id=\"650a23d1\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-71fb0b4 elementor-widget elementor-widget-text-editor\" data-id=\"71fb0b4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">Don&#8217;t find the product you need? Contact us directly.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1760f951 e-con-full e-flex e-con e-child\" data-id=\"1760f951\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-112a86e7 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"112a86e7\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewBox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Contact Us<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Prosep Protein A Affinity HPLC Column For mAb HPLC Analysis Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Direct use on HPLC instrument Fast and accurate analysis of antibody concentration [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2186","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/en\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"Prosep Protein A Affinity HPLC Column For mAb HPLC Analysis Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Direct use on HPLC instrument Fast and accurate analysis of antibody concentration&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Biovanix Protein A affinity HPLC column is for mAb analysis. Stainless steel and PEEK columns with 2.1-30 &amp; 4.6-50mm. 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