{"id":5133,"date":"2025-02-27T14:38:12","date_gmt":"2025-02-27T14:38:12","guid":{"rendered":"https:\/\/biovanix.com\/?p=5133"},"modified":"2025-02-27T15:00:52","modified_gmt":"2025-02-27T15:00:52","slug":"one-step-purification-for-live-attenuated-rabies-virus-vaccine","status":"publish","type":"post","link":"https:\/\/biovanix.com\/en\/one-step-purification-for-live-attenuated-rabies-virus-vaccine\/","title":{"rendered":"One-step\u00a0Purification For Live Attenuated Rabies Virus Vaccine"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"5133\" class=\"elementor elementor-5133\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c72fc52 e-flex e-con-boxed e-con e-parent\" data-id=\"c72fc52\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b0a4716 elementor-widget elementor-widget-heading\" data-id=\"b0a4716\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">One-step Purification For Live Attenuated Rabies Virus Vaccine<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-02ded2e elementor-widget elementor-widget-text-editor\" data-id=\"02ded2e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (<a href=\"https:\/\/www.cdc.gov\/rabies\/about.html\">CDC<\/a>). In up to 99% of cases, domestic dogs are responsible for rabies virus transmission to humans. Yet, rabies can affect both domestic and wild animals. It is spread to people through bites or scratches, usually via saliva. Without rabies vaccine, the fatality rate is nearly 100% fatality rate when humans and pets are infected. (<a href=\"https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/rabies\">World Health Organization<\/a>). Rabies vaccines are the most effective method to protect human and pets when they are exposed.<\/p><p>Compare with traditional rabies vaccine purification (membrane separation and size exclusion), Biovanix Biopure AF media can purify the live attenuated rabies virus just in one step with better virus protein recovery and purity.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7a4790f e-flex e-con-boxed e-con e-parent\" data-id=\"7a4790f\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-dd7ccf0 elementor-widget elementor-widget-text-editor\" data-id=\"dd7ccf0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Rabies Vaccine Purification Tests<\/h2><h3>Result<\/h3><ul><li>Recovery &gt; 80%<\/li><li>Purity &gt; 90%<\/li><li>Removal rate of impurities &gt; 99%<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-94a9f8d e-flex e-con-boxed e-con e-parent\" data-id=\"94a9f8d\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-c71e931 e-con-full e-flex e-con e-child\" data-id=\"c71e931\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e52b74a elementor-widget elementor-widget-text-editor\" data-id=\"e52b74a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><b>Test Condition:<\/b><\/p><ul><li>Column: \u00a0VirCap\u00ae AF 1ml prepacked column<\/li><li>Mobile phase A: 10mM PB pH7.4<\/li><li>Mobile phase B: 10mM PB pH7.4 2M NaCl<\/li><li>Flow Rate: 0.5ml\/min<\/li><li>Testing: UV 280nm<\/li><li>Sample: Clarified virus medium<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-f851655 e-con-full e-flex e-con e-child\" data-id=\"f851655\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a8953a8 elementor-widget elementor-widget-image\" data-id=\"a8953a8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"174\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-300x174.jpg\" class=\"attachment-medium size-medium wp-image-5118\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-300x174.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-768x444.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1-600x347.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biopure-perfusion-chromatography-AF-application-1.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2a32318 e-flex e-con-boxed e-con e-parent\" data-id=\"2a32318\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-95f020d elementor-widget elementor-widget-text-editor\" data-id=\"95f020d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Using HPSEC analysis for the tests of sample solution, percolation fluid and collection fluid, and no peak of target in breakthough at RT=10min. Biopure AF media elutes virus particles by changing the ion strength in mobile phase. Both the purity and concentration of the virus improves significantly.<\/p><p>Parallel\u00a0tests of HPSEC analysis for target peak collection is also done among competitors.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-37c9f2e e-flex e-con-boxed e-con e-parent\" data-id=\"37c9f2e\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-0918eb5 e-con-full e-flex e-con e-child\" data-id=\"0918eb5\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cda7b79 elementor-widget elementor-widget-image\" data-id=\"cda7b79\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"169\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-169x300.jpg\" class=\"attachment-medium size-medium wp-image-5134\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-169x300.jpg 169w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification.jpg 338w\" sizes=\"(max-width: 169px) 100vw, 169px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-db14fdc e-con-full e-flex e-con e-child\" data-id=\"db14fdc\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-94d95b0 elementor-widget elementor-widget-image\" data-id=\"94d95b0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"181\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-300x181.jpg\" class=\"attachment-medium size-medium wp-image-5135\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-300x181.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-768x463.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1-600x362.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/02\/biovanix-one-step-purification-1.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a85ce24 e-flex e-con-boxed e-con e-parent\" data-id=\"a85ce24\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-d57dad7 elementor-widget elementor-widget-text-editor\" data-id=\"d57dad7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-module fl-module-rich-text fl-node-5e68a113ddd36\" data-node=\"5e68a113ddd36\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h2><b>CIP and Clearance<\/b><\/h2><p>Frequency of Clean-in-Place (CIP) depends on properties and conditions of solvent.<\/p><p>For the capture step, we suppose at least 30 minutes of 1 M NaOH countercurrent CIP process after each running.<\/p><p>To reduce microbial contamination in the prepacked column, clean with 0.5 to 1.0 M NaOH, 1 h contact time is recommended.<\/p><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-609b908faae17\" data-node=\"609b908faae17\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h3>Another Purification Method For Rabies Virus<\/h3><p><a href=\"http:\/\/galaklc.com\/chromatography-purification-of-inactive-rabies-virus\/\">Chromatography Purification of Inactive Rabies Virus<\/a><\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). In up to 99% of cases, domestic dogs are responsible for rabies [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-5133","post","type-post","status-publish","format-standard","hentry","category-blog"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/en\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). In up to 99% of cases, domestic dogs are responsible for rabies&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"One-step Purification For Live Attenuated Rabies Virus Vaccine Rabies is a preventable viral disease most often transmitted through the bite of a rabid animal. The rabies virus infects the central nervous system of mammals, ultimately causing disease in the brain and death (CDC). 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