{"id":4865,"date":"2025-02-08T06:38:22","date_gmt":"2025-02-08T06:38:22","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4865"},"modified":"2025-02-08T06:42:49","modified_gmt":"2025-02-08T06:42:49","slug":"proteina-a50-ps-7008-0b-28-400","status":"publish","type":"page","link":"https:\/\/biovanix.com\/es\/protein-a50-psdvb-affinity-media\/","title":{"rendered":"Prote\u00edna A50 PSDVB Medios de afinidad"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"4865\" class=\"elementor elementor-4865\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-873fbec e-flex e-con-boxed e-con e-parent\" data-id=\"873fbec\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0296c3c elementor-widget elementor-widget-heading\" data-id=\"0296c3c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Resina de cromatograf\u00eda de afinidad de PSDVB con Prote\u00edna A50<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53bd545 elementor-widget elementor-widget-text-editor\" data-id=\"53bd545\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>El medio de cromatograf\u00eda de afinidad Prote\u00edna A50 est\u00e1 dise\u00f1ado para analizar y purificar anticuerpos monoclonales (mAbs). En comparaci\u00f3n con los medios de agarosa tradicionales, la Prote\u00edna A50 ofrece ventajas como una alta capacidad de uni\u00f3n din\u00e1mica (DBC), una larga vida \u00fatil y una menor lixiviaci\u00f3n del ligando. Se puede utilizar NaOH (0,1-0,5 M) para la limpieza in situ (CIP).<br \/>El ligando de la Prote\u00edna A50 es prote\u00edna A recombinante (rProte\u00edna A) inmovilizada en la superficie del sustrato de microsferas PS\/DVB macroporosas. La rProte\u00edna A tiene una mejor capacidad de resistencia a los \u00e1lcalis que garantiza estabilidad en condiciones de pH alto. Con nuestro tratamiento hidrof\u00edlico y tecnolog\u00eda de uni\u00f3n, eliminamos la uni\u00f3n inespec\u00edfica a la superficie de PS-DVB. Por lo tanto, la Prote\u00edna A50 es extremadamente \u00fatil para el proceso de purificaci\u00f3n de anticuerpos monoclonales.<\/p><p><strong>Ventajas:<\/strong><\/p><ul><li>Matriz monodispersa de alta rigidez, baja contrapresi\u00f3n, adecuada para procesamiento a gran escala.<\/li><li>Capacidad de uni\u00f3n din\u00e1mica excepcional con un tiempo de residencia bajo que reduce el tiempo del proceso y la cantidad de medio utilizado<\/li><li>Un ligando de Prote\u00edna A novedoso permite el uso de 0.1-0.5 M de NaOH para la desinfecci\u00f3n in situ (CIP).<\/li><li>Baja fuga de ligando asegura una larga vida \u00fatil<\/li><li class=\"nitro-offscreen\">Alta relaci\u00f3n precio-rendimiento<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3db3010 e-flex e-con-boxed e-con e-parent\" data-id=\"3db3010\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b5fa2a0 elementor-widget elementor-widget-heading\" data-id=\"b5fa2a0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Aplicaciones<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23ee177 elementor-widget elementor-widget-text-editor\" data-id=\"23ee177\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Purificaci\u00f3n de prote\u00ednas recombinantes.<\/li><li>Separaci\u00f3n de enzimas y anticuerpos.<\/li><li>Captura de His-Tag, GST y otras prote\u00ednas de fusi\u00f3n.<\/li><li>Producci\u00f3n biofarmac\u00e9utica a gran escala.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-213570e e-flex e-con-boxed e-con e-parent\" data-id=\"213570e\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ee27bea elementor-widget elementor-widget-heading\" data-id=\"ee27bea\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Caracter\u00edsticas principales<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f3360bb elementor-widget elementor-widget-text-editor\" data-id=\"f3360bb\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-row fl-row-fixed-width fl-row-bg-color fl-node-5dd0ec099131e fl-row-default-height fl-row-align-center nitro-offscreen\" data-node=\"5dd0ec099131e\"><div class=\"fl-row-content-wrap\"><div class=\"fl-row-content fl-row-fixed-width fl-node-content\"><div class=\"fl-col-group fl-node-5dd0ec0993e26\" data-node=\"5dd0ec0993e26\"><div class=\"fl-col fl-node-5dd0ec0993ece fl-col-bg-color\" data-node=\"5dd0ec0993ece\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-rich-text fl-node-5dd0eb9979558\" data-node=\"5dd0eb9979558\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><div class=\"fusion-text\"><table style=\"font-size: 16px;\" width=\"609\"><tbody><tr><td width=\"200\"><b>Matriz de apoyo<\/b><\/td><td width=\"409\">Poli(estireno\/divinilbenceno) (PE-DVB)<\/td><\/tr><tr><td width=\"200\"><b>Ligando<\/b><\/td><td width=\"409\">Prote\u00edna A recombinante<\/td><\/tr><tr><td width=\"200\"><b>Ave.\u00a0<\/b><b>Tama\u00f1o de part\u00edcula<\/b><\/td><td width=\"409\">50\u00b5m<\/td><\/tr><tr><td width=\"200\"><b>Capacidad de encuadernaci\u00f3n din\u00e1mica<\/b>\u00a0<b>(<\/b><b>DBC)<\/b><\/td><td width=\"409\">Aprox. 40 mg de IgG humana\/ml de medio (determinado en el avance de 10% mediante an\u00e1lisis frontal a una velocidad de la fase m\u00f3vil de 500 cm\/h en una columna con una altura de lecho de 5 cm; tiempo de residencia: 0,6 min)<\/td><\/tr><tr><td width=\"200\"><b>Reducci\u00f3n\/Expansi\u00f3n<\/b><\/td><td width=\"409\">&lt; 1% de 1-100% disolvente org\u00e1nico<\/td><\/tr><tr><td width=\"200\"><b>Rango de pH (largo plazo)<\/b><\/td><td width=\"409\">pH 2-10<\/td><\/tr><tr><td width=\"200\"><b>Presi\u00f3n m\u00e1xima de operaci\u00f3n<\/b><\/td><td width=\"409\">1500 psi (100 bar \/ 10 MPa)<\/td><\/tr><tr><td width=\"200\"><b>Agentes de limpieza<\/b><\/td><td width=\"409\">0.1-0.5M NaOH<\/td><\/tr><tr><td width=\"200\"><b>Estabilidad de temperatura<\/b><\/td><td width=\"409\">4-40 \u00b0C<\/td><\/tr><tr><td width=\"200\"><b>Condiciones de entrega<\/b><\/td><td width=\"409\">Etanol 20% (2-8 \u2103)<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-60ebf4829784c fl-row-default-height fl-row-align-center nitro-offscreen\" data-node=\"60ebf4829784c\"><div class=\"fl-row-content-wrap\"><div class=\"fl-row-content fl-row-fixed-width fl-node-content\"><div class=\"fl-col-group fl-node-60ebf482b3ab5\" data-node=\"60ebf482b3ab5\"><div class=\"fl-col fl-node-60ebf482b3b68 fl-col-bg-color\" data-node=\"60ebf482b3b68\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-spacer-gap fl-node-60ebf48296c05\" data-node=\"60ebf48296c05\"><div class=\"fl-module-content fl-node-content\">\u00a0<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a0bb558 e-flex e-con-boxed e-con e-parent\" data-id=\"a0bb558\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-285d2c1 elementor-widget elementor-widget-heading\" data-id=\"285d2c1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u00bfPor qu\u00e9 elegir la resina de cromatograf\u00eda de afinidad PSDVB?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b43c658 elementor-widget elementor-widget-text-editor\" data-id=\"b43c658\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong>1. Tama\u00f1o de part\u00edcula<\/strong><\/h3><ul><li><strong>Tama\u00f1o de part\u00edcula:<\/strong>\u00a030 \u03bcm \u2013 100 \u03bcm (personalizable).<\/li><li><strong>Distribuci\u00f3n uniforme:<\/strong>\u00a0Asegura un flujo constante y una separaci\u00f3n de alta resoluci\u00f3n.<\/li><\/ul><h3><strong>2. Estructura porosa<\/strong><\/h3><ul><li><strong>Tama\u00f1o de poro:<\/strong>\u00a0Optimizado para el tama\u00f1o de la prote\u00edna objetivo, asegurando una transferencia de masa eficiente.<\/li><li><strong>Alta porosidad<\/strong>\u00a0Maximiza el \u00e1rea de superficie y la capacidad de carga.<\/li><\/ul><h3><strong>3. Qu\u00edmica de Superficies<\/strong><\/h3><ul><li><strong>Alta \u00e1rea superficial:<\/strong>\u00a0\u00c1rea superficial BET &gt; 500 m\u00b2\/g.<\/li><li><strong>Uni\u00f3n M\u00ednima No Espec\u00edfica<\/strong>\u00a0Menor interferencia de fondo para una eluci\u00f3n m\u00e1s limpia.<\/li><\/ul><h3><strong>4. Propiedades del ligando de afinidad<\/strong><\/h3><ul><li><strong>Tipos de ligandos:<\/strong>\u00a0Opciones personalizables (por ejemplo, His-Tag, MBP, Strep Tag).<\/li><li><strong>Densidad del ligando:<\/strong>\u00a0&gt;10 \u03bcmol\/mL, asegurando una alta capacidad de uni\u00f3n.<\/li><\/ul><h3><strong>5. Capacidad de Carga<\/strong><\/h3><ul><li><strong>Capacidad de enlace din\u00e1mico:<\/strong>\u00a0Hasta 50 mg\/mL en condiciones de alto flujo.<\/li><li><strong>Alto rendimiento<\/strong>\u00a0Recuperaci\u00f3n de prote\u00ednas consistente y confiable.<\/li><\/ul><h3><strong>6. Resistencia Mec\u00e1nica<\/strong><\/h3><ul><li><strong>Alta resistencia a la compresi\u00f3n:<\/strong>\u00a0&gt;10 MPa, lo que garantiza una estabilidad a largo plazo bajo presi\u00f3n.<\/li><\/ul><h3><strong>7. Durabilidad qu\u00edmica<\/strong><\/h3><ul><li><strong>Estabilidad del pH:<\/strong>\u00a0Opera efectivamente en un amplio rango de pH (2\u201312).<\/li><li><strong>Resistencia a solventes org\u00e1nicos:<\/strong>\u00a0Estable en altas concentraciones de acetonitrilo y etanol.<\/li><\/ul><h3><strong>8. Estabilidad t\u00e9rmica<\/strong><\/h3><ul><li><strong>Resistencia al calor<\/strong>\u00a0Estable a temperaturas de hasta 80\u00b0C, lo que permite una limpieza y esterilizaci\u00f3n eficientes.<\/li><\/ul><h3><strong>9. Consistencia de Lote a Lote<\/strong><\/h3><ul><li><strong>Rendimiento uniforme<\/strong>\u00a0Asegura una eficiencia de separaci\u00f3n constante entre diferentes lotes de producci\u00f3n.<\/li><\/ul><h3><strong>10. Eficiencia de costos<\/strong><\/h3><ul><li><strong>Uso Econ\u00f3mico:<\/strong>\u00a0Alta capacidad de carga y larga vida \u00fatil reducen los costos generales de producci\u00f3n.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-16f64849 e-flex e-con-boxed e-con e-parent\" data-id=\"16f64849\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-3e827943 e-con-full e-flex e-con e-child\" data-id=\"3e827943\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5be8f86a elementor-widget elementor-widget-text-editor\" data-id=\"5be8f86a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">\u00bfNo encuentra el producto que necesita? P\u00f3ngase en contacto con nosotros directamente.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-66a5552 e-con-full e-flex e-con e-child\" data-id=\"66a5552\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4214d054 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"4214d054\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">P\u00f3ngase en contacto con nosotros<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).The ligand of Protein [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4865","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"Biovanix","author_link":"https:\/\/biovanix.com\/es\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).The ligand of Protein&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.6.2 - aioseo.com -->\n\t<meta name=\"description\" content=\"Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. 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