{"id":4878,"date":"2025-02-08T13:13:16","date_gmt":"2025-02-08T13:13:16","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4878"},"modified":"2026-09-15T12:03:41","modified_gmt":"2026-09-15T04:03:41","slug":"resina-de-cromatografia-de-afinidad-oligo-dt25","status":"publish","type":"page","link":"https:\/\/biovanix.com\/es\/oligo-dt25-affinity-chromatography-resin\/","title":{"rendered":"Oligo dT(25) Resina de cromatograf\u00eda de afinidad"},"content":{"rendered":"
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Oligo dT(25) Resina de cromatograf\u00eda de afinidad<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Resina de afinidad Biovanix Oligo dT(25)<\/strong>\u00a0es una soluci\u00f3n de vanguardia dise\u00f1ada para la purificaci\u00f3n y el aislamiento de ARNm a partir de procesos de fabricaci\u00f3n de transcripci\u00f3n in vitro (TIV). Esta resina avanzada captura selectivamente el ARNm a trav\u00e9s de su cola poliadenilada (poliA), separ\u00e1ndolo eficazmente de otros componentes de la reacci\u00f3n de transcripci\u00f3n, como las enzimas y el ADN plasm\u00eddico.<\/p>

Perfectamente adecuado para la purificaci\u00f3n a gran escala,\u00a0Resina de afinidad Biovanix Oligo dT(25)<\/strong>\u00a0es un factor clave para las aplicaciones de vacunas y terapia g\u00e9nica, ya que ofrece una selectividad y una capacidad inigualables para satisfacer las exigencias de la fabricaci\u00f3n biofarmac\u00e9utica moderna.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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50 \u03bcm<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Tama\u00f1o de las part\u00edculas<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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200 nm<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Tama\u00f1o de poro<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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pH 2\u201313<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Stability Range<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Presi\u00f3n m\u00e1xima<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Stability Range<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Caracter\u00edsticas del producto<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Engineered for High-Performance mRNA Purification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Biovanix Oligo dT(25) Affinity Resin combines selective polyA capture with a rigid PS-DVB matrix for reliable results at any scale.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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1. Captura selectiva de ARNm<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 PolyA Tail Specificity:<\/strong>\u00a0Se dirige al ARNm con gran selectividad, garantizando la eliminaci\u00f3n eficaz del ADN plasm\u00eddico y otros componentes de la IVT.<\/p>

\u2714Simple Salt and Water Elution:<\/strong>\u00a0Proceso de purificaci\u00f3n racionalizado con una complejidad m\u00ednima.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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2. Soluci\u00f3n escalable<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Excelente escalabilidad:<\/strong>\u00a0Dise\u00f1ada para pasar sin problemas de la producci\u00f3n a escala de laboratorio a la producci\u00f3n a gran escala.<\/p>

\u2714 High Capacity: <\/strong>Satisface las exigencias de la fabricaci\u00f3n de ARNm de alto rendimiento.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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3. Derivados no animales<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Proceso de fabricaci\u00f3n sint\u00e9tico:<\/strong>\u00a0Los oligoligandos Biovanix no contienen componentes animales, lo que garantiza el cumplimiento de los requisitos reglamentarios y las normas \u00e9ticas.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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4. Flujo de trabajo simplificado<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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\u2714 <\/strong>Complejidad reducida:<\/strong>\u00a0Elimina la necesidad de m\u00faltiples pasos de purificaci\u00f3n, maximizando la eficiencia y reduciendo la complejidad del procesamiento posterior.<\/p>

\u2714 <\/strong>Rendimiento constante:<\/strong>\u00a0Resultados fiables en diferentes escalas y lotes de producci\u00f3n.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\n\t\t\t\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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How It Works<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Simple 4-Step mRNA Purification Workflow<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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From crude IVT mixture to purified mRNA in a single affinity capture step \u2014 no complex multi-step protocols required.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2460<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Equilibrate<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Prepare column with high-salt binding buffer (e.g., 0.5 M NaCl)<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2461<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Load & Bind<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Apply IVT mixture; polyA-tailed mRNA binds to poly(dT) ligands<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2462<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Wash<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Remove impurities (enzymes, pDNA, nucleotides) in flow-through<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2463<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Elute<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Release purified mRNA with water or low-salt buffer<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Technical Specifications<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Product Specifications<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Comprehensive technical data for method development and process validation.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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Caracter\u00edstica<\/strong><\/td>Descripci\u00f3n<\/strong><\/td><\/tr>
Matriz de apoyo<\/strong><\/td>Poli(estireno-divinilbenceno) reticulado<\/td><\/tr>
Tama\u00f1o medio de las part\u00edculas<\/strong><\/td>50 um<\/td><\/tr>
Tama\u00f1o medio de los poros<\/strong><\/td>200 nm<\/td><\/tr>
Funcionalidad de la superficie<\/strong><\/td>poly(dT) 25mer con enlazador patentado<\/td><\/tr>
Densidad del ligando<\/strong><\/td>0,3 umol\/ml<\/td><\/tr>
Resistencia mec\u00e1nica<\/strong><\/td>70 bar (1.000 psi; 7 MPa)<\/td><\/tr>
Estabilidad t\u00e9rmica<\/strong><\/td>permite la desnaturalizaci\u00f3n de la muestra a 65\u00b0C si es necesario<\/td><\/tr>
Rango de pH<\/strong><\/td>2-13<\/td><\/tr>
Gama de fuerza i\u00f3nica<\/strong><\/td>0 a 5 M, todas las sales comunes<\/td><\/tr>
Resistencia qu\u00edmica<\/strong><\/td>Los agentes comunes para la purificaci\u00f3n de ARNm, incluyen NaOH 0,5 M, 2 M MgCl2, 20 mM EDTA. Agua, alcohol de 0 a 100%, acetonitrilo, \u00e1cido ac\u00e9tico 2 M, HCl 1 M y otros disolventes org\u00e1nicos comunes.<\/td><\/tr>
Almacenamiento<\/strong><\/td>18-20% etanol<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Resistencia qu\u00edmica y t\u00e9rmica<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Rango de pH<\/strong><\/td>2-13<\/td><\/tr>
Gama de fuerza i\u00f3nica<\/strong><\/td>0 a 5 M, todas las sales comunes<\/td><\/tr>
Aditivos tamp\u00f3n<\/strong><\/td>

Los agentes comunes para la purificaci\u00f3n de ARNm incluyen NaOH 0,5 M, 2 MMgCl2, 20 mM EDTA.<\/p>

No exponer a oxidantes fuertes (como el hipoclorito), \u00e1cidos oxidantes (como el n\u00edtrico), agentes reductores fuertes (como el sulfito), acetona, THF o alcohol benc\u00edlico.<\/p><\/td><\/tr>

Disolventes<\/strong><\/td>

Agua, alcohol de 0 a 100%, acetonitrilo, \u00e1cido ac\u00e9tico 2 M, HCl 1 M y otros disolventes org\u00e1nicos comunes.<\/p>

No exponer a oxidantes fuertes (como el hipoclorito), \u00e1cidos oxidantes (como el n\u00edtrico), agentes reductores fuertes (como el sulfito), acetona, alcohol benc\u00edlico o THF.<\/p><\/td><\/tr>

Caudal<\/strong><\/td>Ajustar el caudal en funci\u00f3n del rendimiento. No sobrepasar los l\u00edmites superiores de presi\u00f3n.<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Resumen de prestaciones<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Why Biovanix Oligo dT(25) Resin<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Designed to maximize purity, simplify workflows, and reduce total cost of ownership.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u26a1<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Alta eficacia<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Purificaci\u00f3n r\u00e1pida y eficaz del ARNm.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udcc8<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Proceso escalable<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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F\u00e1cilmente adaptable a la fabricaci\u00f3n a gran escala.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udee1\ufe0f<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Cumplimiento de la normativa<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Proceso de fabricaci\u00f3n no derivado de animales y sint\u00e9tico.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\ud83d\udcb0<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Rentable<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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La simplificaci\u00f3n del flujo de trabajo reduce la complejidad operativa y los costes.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Aplicaciones<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Built for the mRNA Revolution<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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From vaccine development to gene therapy manufacturing, Biovanix Oligo dT(25) Resin supports the full spectrum of mRNA applications.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Producci\u00f3n de vacunas<\/h3>

Purificaci\u00f3n eficaz de vacunas de ARNm.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Terapia g\u00e9nica<\/h3>

Producci\u00f3n escalable de ARNm para aplicaciones terap\u00e9uticas.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Fabricaci\u00f3n IVT<\/h3>

Aislamiento racionalizado de ARNm a partir de mezclas de reacci\u00f3n de transcripci\u00f3n.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Extracci\u00f3n de ADN plasm\u00eddico<\/h3>

Eliminaci\u00f3n eficaz del ADN plasm\u00eddico y otros componentes de la IVT.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Why Choose Biovanix<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Your Trusted Partner in mRNA Purification<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\u2460\u00a0Efficient mRNA Purification<\/h3>

Obtenga ARNm de alta pureza con impurezas m\u00ednimas.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2461\u00a0Cost-Effective<\/h3>

La reducci\u00f3n de la complejidad operativa y el aumento de la productividad reducen los costes generales de producci\u00f3n.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2462 Regulatory Compliance<\/h3>

La fabricaci\u00f3n no derivada de animales y sint\u00e9tica garantiza el cumplimiento de las normas mundiales.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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\u2463\u00a0Versatile Application<\/h3>

Ideal para una amplia gama de construcciones de ARNm utilizadas en vacunas, terapias g\u00e9nicas y otros productos biofarmac\u00e9uticos.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Frequently Asked Questions<\/h4>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Oligo dT(25) Resin FAQ<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Common questions about mRNA purification with Oligo dT(25) affinity chromatography.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t

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What is Oligo dT(25) affinity chromatography resin and how does it work? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Oligo dT(25) affinity chromatography resin is a specialized chromatography medium functionalized with poly(deoxythymidine) 25-mer ligands attached to a solid support matrix. It works through complementary A-T base pairing: the poly(dT) ligands on the resin selectively bind to the polyadenylated (polyA) tail of mRNA molecules. When an IVT (in vitro transcription) mixture is loaded onto the column under high-salt conditions, mRNA is captured while impurities such as enzymes, plasmid DNA, and unincorporated nucleotides pass through. Elution is achieved with water or low-salt buffer, releasing the purified mRNA.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the principle behind mRNA purification using oligo dT affinity chromatography? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The purification principle relies on specific Watson-Crick A-T base pairing between the poly(dT) 25-mer ligand immobilized on the resin and the polyA tail present on mature mRNA. Under high ionic strength conditions (typically 0.5 M NaCl), electrostatic shielding stabilizes the hybridization between poly(dT) and polyA. The mRNA binds selectively to the resin while process impurities flow through. Elution is performed under low-ionic-strength conditions (water or dilute buffer), which destabilizes the hybrid and releases purified mRNA in a single chromatographic step.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What types of mRNA can be purified with Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Oligo dT(25) resin can purify any polyadenylated mRNA produced through in vitro transcription (IVT), including mRNA constructs for vaccines (such as infectious disease vaccines), gene therapy mRNAs (such as those encoding therapeutic proteins or gene editing components), and research-grade mRNAs of various lengths. The resin is compatible with mRNA constructs ranging from short transcripts to several thousand nucleotides in length, as long as they contain a polyA tail.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the binding capacity of Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The binding capacity of Oligo dT(25) resin depends on the mRNA construct length, loading conditions, and the specific product formulation. Generally, affinity resins with poly(dT) ligands offer high dynamic binding capacity suitable for preparative-scale mRNA purification. Smaller mRNA constructs tend to achieve higher binding capacity per mL of resin due to reduced steric hindrance. For specific capacity data for your mRNA construct, contact the Biovanix technical team for application-specific recommendations.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What are the advantages of PS-DVB matrix vs. agarose for mRNA purification? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Poly(styrene-divinylbenzene) (PS-DVB) matrices offer several advantages over traditional agarose gels for mRNA purification: (1) Higher mechanical strength \u2014 PS-DVB beads are rigid, enabling higher flow rates and pressure tolerance (up to 70 bar for Biovanix resin) without bed compression; (2) Broader chemical compatibility \u2014 stable across pH 2\u201313 and resistant to organic solvents, enabling aggressive cleaning-in-place (CIP) with NaOH; (3) Longer resin lifetime \u2014 the rigid polymer backbone does not compress or degrade over repeated cycles; (4) Better scalability \u2014 linear pressure-flow behavior allows consistent performance from lab to production scale; (5) No risk of gel compression artifacts during scale-up.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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How do I pack and use Oligo dT(25) resin in my chromatography system? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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General workflow: (1) Equilibrate the column with binding buffer (high-salt buffer, e.g., 0.5 M NaCl) to prepare the resin for mRNA capture; (2) Load the IVT reaction mixture onto the column \u2014 the mRNA polyA tail binds to the poly(dT) ligands while impurities flow through; (3) Wash the column with binding buffer or a moderate-salt wash buffer to remove residual impurities; (4) Elute purified mRNA with water or low-salt buffer (e.g., 10 mM Tris-HCl); (5) Clean and regenerate the column with appropriate CIP solution (e.g., 0.1\u20130.5 M NaOH) for reuse. Always adjust flow rate to stay within the pressure limit (70 bar maximum for Biovanix resin). For denaturing secondary structures, samples can be heated to 65\u00b0C during processing.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What flow rate and pressure should I use with Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Biovanix Oligo dT(25) resin has a maximum mechanical resistance of 70 bar (1,000 psi \/ 7 MPa). The flow rate should be adjusted based on your specific application and column dimensions, ensuring that backpressure does not exceed this limit. The 50 \u03bcm average particle size allows efficient mass transfer at moderate to high linear flow rates. Start at a conservative flow rate during method development and optimize based on binding efficiency and recovery. The rigid PS-DVB matrix maintains stable bed volume across flow rate changes, providing consistent performance.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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Can I reuse the Oligo dT(25) resin and how do I clean it? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Yes, Oligo dT(25) resin can be reused for multiple purification cycles with proper cleaning and storage. Cleaning-in-place (CIP) is typically performed using 0.1\u20130.5 M NaOH, which effectively removes residual nucleic acids and contaminants. The resin’s broad pH stability (2\u201313) and chemical resistance support stringent cleaning protocols. After CIP, re-equilibrate the column with binding buffer before the next use. Store the resin in 18\u201320% ethanol at 2\u20138\u00b0C when not in use. Do not freeze. Resin lifetime depends on the application, cleaning protocol, and feed material quality.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What buffer conditions are recommended for mRNA binding and elution? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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For mRNA binding (capture): use a high-salt buffer such as 0.5 M NaCl with an appropriate buffer component (e.g., 10 mM Tris-HCl, pH 7.5). The high ionic strength provides electrostatic shielding that stabilizes the A-T hybridization between poly(dT) ligands and the mRNA polyA tail. For washing: use the same high-salt binding buffer or a moderately reduced salt concentration. For elution: use deionized water or a low-salt buffer (e.g., 10 mM Tris-HCl without NaCl) to destabilize the hybrid and release purified mRNA. The resin also allows sample denaturing at 65\u00b0C if needed to eliminate undesired higher-order RNA structures.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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How does Oligo dT(25) resin remove plasmid DNA and other IVT impurities? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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The selectivity of Oligo dT(25) resin is based on the specific A-T base pairing between the poly(dT) ligands and the polyA tail of mRNA. Only polyadenylated mRNA molecules bind to the resin. Plasmid DNA (which lacks a polyA tail), RNA polymerase enzymes, unincorporated nucleotides, short abortive transcripts, and buffer components do not bind and are removed in the flow-through and wash fractions. This single-step affinity capture effectively separates full-length mRNA from the major IVT process impurities, significantly reducing downstream processing complexity.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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Can Oligo dT(25) resin be used for continuous or automated mRNA purification? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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Yes, Oligo dT(25) resin is compatible with standard chromatography systems and can be adapted for automated and continuous processing workflows. The rigid PS-DVB matrix provides consistent pressure-flow behavior, making it suitable for use on automated chromatography platforms. The simple salt-and-water elution workflow minimizes buffer exchange requirements. For process development and screening, smaller column formats can be used to optimize conditions before scaling to larger automated systems. The linear scalability of the PS-DVB matrix ensures that conditions optimized at small scale translate directly to production scale.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/details>\n\t\t\t\t\t\t

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What is the shelf life and storage condition for Oligo dT(25) resin? <\/div><\/span>\n\t\t\t\t\t\t\t\n\t\t\t<\/path><\/svg><\/span>\n\t\t\t<\/path><\/svg><\/span>\n\t\t<\/span>\n\n\t\t\t\t\t\t<\/summary>\n\t\t\t\t
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