{"id":3785,"date":"2025-01-20T14:05:32","date_gmt":"2025-01-20T14:05:32","guid":{"rendered":"https:\/\/biovanix.com\/?p=3785"},"modified":"2025-01-20T14:28:42","modified_gmt":"2025-01-20T14:28:42","slug":"como-elegir-una-columna-hplc-de-fase-inversa","status":"publish","type":"post","link":"https:\/\/biovanix.com\/es\/how-to-choose-reversed-phase-hplc-column\/","title":{"rendered":"C\u00f3mo elegir la columna de HPLC de fase inversa"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"3785\" class=\"elementor elementor-3785\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-262a64d e-flex e-con-boxed e-con e-parent\" data-id=\"262a64d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-fe305ec elementor-widget elementor-widget-text-editor\" data-id=\"fe305ec\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1><span style=\"color: #33615a;\">\u00bfC\u00f3mo elegir las columnas de HPLC de fase inversa?<\/span><\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4d04f65 e-flex e-con-boxed e-con e-parent\" data-id=\"4d04f65\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-41ab0ac elementor-widget elementor-widget-text-editor\" data-id=\"41ab0ac\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-module fl-module-rich-text fl-node-5e1be36318c4d\" data-node=\"5e1be36318c4d\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><p>Las distintas fases enlazadas para separaciones en fase inversa presentan diferentes combinaciones de posibles interacciones soluto-fase estacionaria. En el caso de las fases enlazadas, la variedad de mecanismos de separaci\u00f3n influir\u00e1 en la selectividad global. Esta predominancia depender\u00e1 de las propiedades del analito y de las condiciones cromatogr\u00e1ficas aplicadas. Las interacciones m\u00e1s significativas entre el soluto y las fases estacionarias que contribuyen a la selectividad de la columna son las siguientes.<\/p><ul><li><strong>Interacci\u00f3n hidrof\u00f3bica<\/strong>\u00a0es un mecanismo de retenci\u00f3n dominante para todas las columnas de fase inversa y la interacci\u00f3n m\u00e1s significativa de las fases alqu\u00edlicas. Para una fase determinada, el tiempo de retenci\u00f3n es proporcional a la hidrofobicidad de la mol\u00e9cula.<\/li><li><strong>Capacidad de enlace de hidr\u00f3geno<\/strong>\u00a0de una fase implica generalmente la interacci\u00f3n de un grupo b\u00e1sico del soluto con un grupo \u00e1cido dentro de la fase estacionaria, posiblemente a partir de grupos silanol no unidos.<\/li><li><strong>Interacciones \u03c0-\u03c0<\/strong>\u00a0se observan entre un soluto arom\u00e1tico o insaturado y una fase estacionaria arom\u00e1tica.<\/li><li><strong>Selectividad est\u00e9rica<\/strong>\u00a0es una medida de la accesibilidad de los solutos a la fase estacionaria. Las mol\u00e9culas de soluto m\u00e1s grandes pueden quedar excluidas de la fase estacionaria.<\/li><li><strong>Interacciones dipolo-dipolo<\/strong>entre un grupo dipolar del soluto y un grupo dipolar de la fase estacionaria son m\u00e1s importantes en el caso de las columnas de enlace ciano y PFP.<\/li><li><strong>Interacciones de intercambio cati\u00f3nico<\/strong>\u00a0puede producirse entre un soluto cati\u00f3nico y un silanol ionizado dentro de la fase estacionaria.<\/li><\/ul><\/div><\/div><\/div><div class=\"fl-module fl-module-photo fl-node-5e1be6fdad59e\" data-node=\"5e1be6fdad59e\"><div class=\"fl-module-content fl-node-content\">\u00a0<\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-294e000 e-flex e-con-boxed e-con e-parent\" data-id=\"294e000\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1252000 elementor-widget elementor-widget-image\" data-id=\"1252000\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"768\" height=\"231\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection-768x231.webp\" class=\"attachment-medium_large size-medium_large wp-image-3786\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection-768x231.webp 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection-300x90.webp 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection-1024x307.webp 1024w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection-600x180.webp 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/reverse-phase-column-selection.webp 1209w\" sizes=\"(max-width: 768px) 100vw, 768px\" title=\"selecci\u00f3n de columna de fase inversa | BioVanix\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b3abfdb e-flex e-con-boxed e-con e-parent\" data-id=\"b3abfdb\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-25506b9 elementor-widget elementor-widget-text-editor\" data-id=\"25506b9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p class=\"lazyloaded\">A continuaci\u00f3n se ofrece un resumen general de estas interacciones para fases enlazadas de fase inversa t\u00edpicas. Pueden predominar distintas interacciones para distintos analitos y las intensidades de interacci\u00f3n variar\u00e1n seg\u00fan las fases enlazadas de los distintos fabricantes. No obstante, esta tabla ofrece una indicaci\u00f3n \u00fatil de las posibles intensidades de interacci\u00f3n.<\/p><table><tbody><tr><td width=\"56\"><p><strong>Fase de enlace<\/strong><\/p><\/td><td class=\"lazyloaded\" width=\"53\"><strong>Listado USP<\/strong><\/td><td width=\"80\"><strong>Hidr\u00f3fobo<\/strong><\/td><td width=\"61\"><strong>Enlace H<\/strong><\/td><td width=\"57\"><strong>\u03c0-\u03c0<\/strong><\/td><td width=\"61\"><strong>Est\u00e9rico<\/strong><\/td><td width=\"61\"><strong>Dipolo-dipolo<\/strong><\/td><td width=\"64\"><strong>Intercambio cati\u00f3nico<\/strong><\/td><\/tr><tr><td width=\"56\">C18<\/td><td width=\"53\">L1<\/td><td width=\"80\">Muy fuerte<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"57\">No<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"61\">No<\/td><td width=\"64\">D\u00e9bil<\/td><\/tr><tr><td width=\"56\">C8<\/td><td width=\"53\">L7<\/td><td width=\"80\">Fuerte<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"57\">No<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"61\">No<\/td><td width=\"64\">D\u00e9bil<\/td><\/tr><tr><td width=\"56\">C4<\/td><td width=\"53\">L26<\/td><td width=\"80\">D\u00e9bil<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"57\">No<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"61\">No<\/td><td width=\"64\">D\u00e9bil<\/td><\/tr><tr><td width=\"56\">Fenil<\/td><td width=\"53\">L11<\/td><td width=\"80\">Fuerte<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"57\">Donante fuerte<\/td><td width=\"61\">Moderado<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"64\">D\u00e9bil<\/td><\/tr><tr><td width=\"56\">PFP<\/td><td width=\"53\">L43<\/td><td width=\"80\">Moderado<\/td><td width=\"61\">Moderado<\/td><td width=\"57\">Fuerte aceptor<\/td><td width=\"61\">Moderado<\/td><td width=\"61\">Fuerte<\/td><td width=\"64\">Moderado<\/td><\/tr><tr><td width=\"56\">Ciano<\/td><td width=\"53\">L10<\/td><td width=\"80\">D\u00e9bil<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"57\">D\u00e9bil<\/td><td width=\"61\">D\u00e9bil<\/td><td width=\"61\">Fuerte<\/td><td width=\"64\"><p>D\u00e9bil<\/p><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3f0743d e-flex e-con-boxed e-con e-parent\" data-id=\"3f0743d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4224b5c elementor-widget elementor-widget-image\" data-id=\"4224b5c\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" width=\"768\" height=\"156\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis-768x156.webp\" class=\"attachment-medium_large size-medium_large wp-image-3787\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis-768x156.webp 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis-300x61.webp 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis-1024x208.webp 1024w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis-600x122.webp 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2025\/01\/C4C8C18-column-analysis.webp 1500w\" sizes=\"(max-width: 768px) 100vw, 768px\" title=\"C4C8C18-columna-an\u00e1lisis | BioVanix\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-eca007f e-flex e-con-boxed e-con e-parent\" data-id=\"eca007f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8b36387 elementor-widget elementor-widget-text-editor\" data-id=\"8b36387\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-module fl-module-rich-text fl-node-5e1be7cadf753\" data-node=\"5e1be7cadf753\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h1>Columna de fase inversa C18<\/h1><p>La hidrofobicidad es el principal mecanismo de interacci\u00f3n del analito con el C18 y otras fases estacionarias con enlaces alqu\u00edlicos. Adem\u00e1s, la polaridad de la fase tambi\u00e9n contribuir\u00e1 a la selectividad global observada.<\/p><h3>Hidrofobicidad<\/h3><p>La fuerza de la interacci\u00f3n hidr\u00f3foba puede medirse por la retenci\u00f3n de mol\u00e9culas neutras (no polares). Los valores k (factores de retenci\u00f3n) de una especie neutra, para una fase C18 determinada, dar\u00e1n una indicaci\u00f3n de la superficie y la cobertura superficial (densidad del ligando) de la s\u00edlice.<\/p><p>El porcentaje de carbono en el material es una gu\u00eda simplista pero \u00fatil de las caracter\u00edsticas de retenci\u00f3n hidr\u00f3foba de una columna. En la figura 1, esta correlaci\u00f3n imprecisa queda demostrada por el aumento de la retenci\u00f3n observado al aumentar la longitud de la cadena alqu\u00edlica (es decir, la carga de carbono). Este aumento es el resultado de un aumento de la hidrofobicidad de la fase estacionaria. Del mismo modo, se esperar\u00eda un aumento de la retenci\u00f3n al pasar de una fase C18 con una carga de carbono baja a otra con una carga de carbono alta.<\/p><p>La selectividad hidr\u00f3foba puede determinarse a partir de la relaci\u00f3n del factor de retenci\u00f3n entre dos especies neutras. Esta es una mejor medida de la cobertura superficial que el contenido en carbono, ya que el \u00e1rea superficial y la porosidad pueden variar de una s\u00edlice a otra.<\/p><h3>Polaridad<\/h3><p>La segunda propiedad clave de los materiales C18 es su actividad de silanol, a menudo discutida en t\u00e9rminos de polaridad. Esto puede determinarse midiendo la relaci\u00f3n del factor de retenci\u00f3n entre un compuesto b\u00e1sico y uno \u00e1cido. A un pH &gt;7, la capacidad total de intercambio i\u00f3nico corresponder\u00e1 a una medida de la actividad de silanol total. A pH \u00e1cido (por ejemplo, pH 2,7) puede obtenerse una indicaci\u00f3n de la actividad \u00e1cida de los grupos silanol. La presencia de iones met\u00e1licos en la s\u00edlice base aumenta el nivel de actividad del silanol. Las s\u00edlices de generaciones anteriores tienen niveles m\u00e1s elevados y menos controlados de iones met\u00e1licos y, por tanto, mayores<br \/>actividad silanol en comparaci\u00f3n con las fases de enlace alquilo de nueva generaci\u00f3n. Para esta<br \/>y otras razones, se recomienda encarecidamente que el desarrollo de nuevos m\u00e9todos<br \/>debe abordarse utilizando s\u00edlices de mayor pureza de nueva generaci\u00f3n.<\/p><h3>Fases desactivadas de base de gran pureza<\/h3><p>Las fases modernas con enlace alquilo tienen niveles acumulativos de iones met\u00e1licos muy bajos dentro de la s\u00edlice base (&lt;10ppm), lo que hace que el n\u00famero de grupos silanol aislados, y por tanto la polaridad de la superficie de s\u00edlice, tambi\u00e9n se reduzca. Combinados con procesos de enlace m\u00e1s eficaces y reproducibles, estos materiales de fase inversa de nueva generaci\u00f3n permiten mejorar significativamente la cromatograf\u00eda de las mol\u00e9culas de soluto polares m\u00e1s b\u00e1sicas. El uso de grupos alquilo enlazados que contengan sustituyentes hidr\u00f3filos (es decir, incrustados polares) puede potenciar este efecto y\/u ofrecer una selectividad alternativa.<\/p><h3>Optimizaci\u00f3n de la selectividad<\/h3><p>La figura 2 ilustra la relaci\u00f3n entre el cambio de polaridad y la hidrofobicidad de los materiales t\u00edpicos C18, C8 y C4, mostrando una disminuci\u00f3n de la hidrofobicidad al reducir la longitud de la cadena alqu\u00edlica. Una mayor densidad del ligando y, por tanto<br \/>menor polaridad, tambi\u00e9n se observa a medida que se reduce la longitud de la cadena alqu\u00edlica. Sin embargo, cambiar la longitud de la cadena alqu\u00edlica puede reducir el tiempo de an\u00e1lisis, pero no afectar\u00e1 significativamente a la selectividad. El cambio de la qu\u00edmica a una fase enlazada alternativa es una herramienta m\u00e1s potente para conseguirlo.<\/p><h3>Generaci\u00f3n de m\u00e1s edad Fases \"tradicionales<\/h3><p>Las fases C18 \"tradicionales\" m\u00e1s antiguas son hidr\u00f3fobas y tienen una alta polaridad debido a que la s\u00edlice de menor pureza contiene un mayor nivel de grupos silanol \u00e1cidos en los que se basan. El uso de las nuevas s\u00edlices de alta pureza reduce la actividad de silanol de las fases resultantes y mejora la reproducibilidad. El empleo de una funcionalidad polar incrustada tambi\u00e9n puede dar lugar a un material de polaridad reducida. Para solutos b\u00e1sicos que interaccionar\u00e1n fuertemente con los silanoles superficiales, se recomiendan generalmente fases de polaridad m\u00e1s baja. Sin embargo, para determinados an\u00e1lisis, las interacciones adicionales proporcionadas por los silanoles superficiales de un material C18 \"tradicional\" pueden ser beneficiosas para la separaci\u00f3n global.<\/p><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-5e1bfc9080030\" data-node=\"5e1bfc9080030\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h1><strong>Columna de fase inversa de fenilo<\/strong><\/h1><p>Las fases de s\u00edlice con enlaces fen\u00edlicos ofrecen una selectividad en fase inversa alternativa a las fases con enlaces alqu\u00edlicos. Muestran una menor retenci\u00f3n hidr\u00f3foba que sus hom\u00f3logas C18, con caracter\u00edsticas de retenci\u00f3n similares a las fases con enlace C8. Las fases estacionarias fen\u00edlicas interact\u00faan con compuestos que contienen grupos arom\u00e1ticos o enlaces insaturados mediante la participaci\u00f3n de interacciones \u03c0-\u03c0. En el caso de solutos arom\u00e1ticos que contengan un \u00e1tomo o grupo electronegativo (por ejemplo, F, NO2), aumentar\u00e1 el grado de interacciones \u03c0-\u03c0 con la fase fen\u00edlica.<\/p><p>Debido a la naturaleza r\u00edgida del anillo fenilo, la forma del soluto tambi\u00e9n puede influir en la selectividad.<\/p><p>Las fases de fenilo tradicionales tienden a ser menos estables que las correspondientes fases invertidas C8 o C18. Adem\u00e1s, el mayor tama\u00f1o est\u00e9rico del grupo fenilo reduce la cobertura de la superficie, dejando un mayor n\u00famero de sitios de silanol expuestos. Las fases de fenilo introducidas m\u00e1s recientemente muestran una mayor estabilidad. El uso de una base de s\u00edlice m\u00e1s pura, unos procedimientos de uni\u00f3n m\u00e1s eficaces y reproducibles y la disponibilidad de un fenil silano est\u00e9ricamente protegido contribuyen a una mayor robustez de la fase y a una reducci\u00f3n del sangrado de la columna.<\/p><p>Las fases de fenilo convencionales se unen a la s\u00edlice a trav\u00e9s de un espaciador de propilo. La incorporaci\u00f3n del espaciador de hexilo de cadena m\u00e1s larga da lugar a una mayor retenci\u00f3n hidr\u00f3foba y selectividad arom\u00e1tica.<\/p><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-5e1bfd6db0ad3\" data-node=\"5e1bfd6db0ad3\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h1>Fases de s\u00edlice de enlace polar<\/h1><p>Las fases de s\u00edlice con enlaces polares ofrecen una selectividad alternativa a los materiales con enlaces alqu\u00edlicos. En general, tienen menor hidrofobicidad pero mayor polaridad. Las fases de enlace ciano, amino y diol pueden utilizarse tanto en fase normal como en fase inversa. En fase normal, se equilibran m\u00e1s r\u00e1pidamente con el eluyente que la propia s\u00edlice y no se desactivan por trazas de agua.<\/p><p>Las fases de enlace ciano presentan una selectividad \u00fanica para compuestos polares y son m\u00e1s adecuadas que la s\u00edlice desnuda para separaciones en gradiente de fase normal. El grupo funcional ciano es un dipolo fuerte que puede interactuar con otros dipolos o inducir dipolos en los solutos. Estas fases tambi\u00e9n presentan una hidrofobicidad moderada debido al enlazador alquilo.<\/p><p>Las fases con enlace amino presentan una selectividad en fase normal alternativa a la de la s\u00edlice sin enlace, especialmente para arom\u00e1ticos. Las columnas amino tambi\u00e9n se utilizan en el modo HILIC para el an\u00e1lisis de hidratos de carbono y otros compuestos polares. Sus propiedades de intercambio ani\u00f3nico d\u00e9bil pueden utilizarse en el an\u00e1lisis de aniones y \u00e1cidos org\u00e1nicos.<\/p><p>Las fases enlazadas con diol son una alternativa vers\u00e1til a la s\u00edlice no enlazada para separaciones en fase normal. Los grupos hidroxilo proporcionan una buena selectividad sin una retenci\u00f3n excesiva, ya que el enlace H con la capa de diol es m\u00e1s d\u00e9bil que con los silanoles. Algunas fases enlazadas con diol se han desarrollado espec\u00edficamente para aplicaciones HILIC. En las separaciones por exclusi\u00f3n de tama\u00f1o se utilizan materiales con tama\u00f1os de poro diferentes.<\/p><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-766257c e-flex e-con-boxed e-con e-parent\" data-id=\"766257c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-eea07d5 elementor-widget elementor-widget-text-editor\" data-id=\"eea07d5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Columnas de fase inversa y graneles Biovanix<\/h2><table><tbody><tr><td width=\"76\"><strong>\u00a0<\/strong><\/td><td width=\"107\"><strong>Tama\u00f1o de las part\u00edculas<\/strong><\/td><td width=\"90\"><strong>Tama\u00f1o del poste<\/strong><\/td><td width=\"100\"><strong>Superficie<\/strong><\/td><td width=\"92\"><strong>Contenido de carbono<\/strong><\/td><td width=\"86\"><strong>Rango de pH<\/strong><\/td><\/tr><tr><td width=\"76\"><a name=\"_Toc6385822\"><\/a><a name=\"_Toc6385689\"><\/a><strong>C18<\/strong><\/td><td width=\"107\">3\/5\/10\/15\/20\/30\/50\u03bcm<\/td><td width=\"90\">100\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">15%<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>C18BS<\/strong><\/td><td width=\"107\">5\/10\u03bcm<\/td><td width=\"90\">100\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">18%<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>C8<\/strong><\/td><td width=\"107\">3\/5\/10\/15\/20\/30\/50\u03bcm<\/td><td width=\"90\">100\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">12%<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>Diol<\/strong><\/td><td width=\"107\">5\/10\u03bcm<\/td><td width=\"90\">120\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">8%<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>Amino<\/strong><\/td><td width=\"107\">5\/10\u03bcm<\/td><td width=\"90\">120\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">&#8211;<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>CN<\/strong><\/td><td width=\"107\">5\/10\u03bcm<\/td><td width=\"90\">120\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">5%<\/td><td width=\"86\">2-8<\/td><\/tr><tr><td width=\"76\"><strong>NH<sub>2<\/sub><\/strong><\/td><td width=\"107\">5\/10\u03bcm<\/td><td width=\"90\">120\u00c5<\/td><td width=\"100\">300m<sup>2<\/sup>\/g<\/td><td width=\"92\">5%<\/td><td width=\"86\">2-8<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-22cfa8c9 e-flex e-con-boxed e-con e-parent\" data-id=\"22cfa8c9\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-4738cf02 e-con-full e-flex e-con e-child\" data-id=\"4738cf02\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-485a191f elementor-widget elementor-widget-text-editor\" data-id=\"485a191f\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">\u00bfTiene alg\u00fan problema con la cromatograf\u00eda l\u00edquida? P\u00f3ngase directamente en contacto con nosotros.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d1e8d94 e-con-full e-flex e-con e-child\" data-id=\"d1e8d94\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-a83b025 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"a83b025\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">P\u00f3ngase en contacto con nosotros<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>How to choose\u00a0reversed-phase HPLC Columns? Different bonded phases for reversed-phase separations exhibit different combinations of possible solute-stationary phase interactions. For bonded phase, the variety of separation mechanisms will influence the overall selectivity. This predominance depend on the properties of the analyte, and the applied chromatographic conditions. The most significant interactions between solute and stationary phases [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1,49],"tags":[],"class_list":["post-3785","post","type-post","status-publish","format-standard","hentry","category-blog","category-hplc-introduction"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/es\/author\/biovanix-com\/"},"uagb_comment_info":28,"uagb_excerpt":"How to choose\u00a0reversed-phase HPLC Columns? Different bonded phases for reversed-phase separations exhibit different combinations of possible solute-stationary phase interactions. For bonded phase, the variety of separation mechanisms will influence the overall selectivity. This predominance depend on the properties of the analyte, and the applied chromatographic conditions. The most significant interactions between solute and stationary phases&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.6.1 - aioseo.com -->\n\t<meta name=\"description\" content=\"How to choose reversed-phase HPLC Columns? Different bonded phases for reversed-phase separations exhibit different combinations of possible solute-stationary phase interactions. For bonded phase, the variety of separation mechanisms will influence the overall selectivity. This predominance depend on the properties of the analyte, and the applied chromatographic conditions. 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Different bonded phases for reversed-phase separations exhibit different combinations of possible solute-stationary phase interactions. For bonded phase, the variety of separation mechanisms will influence the overall selectivity. This predominance depend on the properties of the analyte, and the applied chromatographic conditions. 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