{"id":3797,"date":"2025-01-20T14:15:33","date_gmt":"2025-01-20T14:15:33","guid":{"rendered":"https:\/\/biovanix.com\/?p=3797"},"modified":"2025-01-20T14:26:36","modified_gmt":"2025-01-20T14:26:36","slug":"how-to-choose-proper-silica-hplc-column","status":"publish","type":"post","link":"https:\/\/biovanix.com\/es\/how-to-choose-proper-silica-hplc-column\/","title":{"rendered":"C\u00f3mo elegir la columna de s\u00edlice adecuada para HPLC"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"3797\" class=\"elementor elementor-3797\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-19664e9 e-flex e-con-boxed e-con e-parent\" data-id=\"19664e9\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2aeab5f elementor-widget elementor-widget-text-editor\" data-id=\"2aeab5f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1><span style=\"color: #33615a;\">How to choose proper silica HPLC column?<\/span><\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-340a0eb e-flex e-con-boxed e-con e-parent\" data-id=\"340a0eb\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-39437b4 elementor-widget elementor-widget-text-editor\" data-id=\"39437b4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-col-group fl-node-5e1d10f001116\" data-node=\"5e1d10f001116\"><div class=\"fl-col fl-node-5e1d10f0011e3 fl-col-bg-color fl-col-has-cols\" data-node=\"5e1d10f0011e3\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-rich-text fl-node-5e1d10eff3aac\" data-node=\"5e1d10eff3aac\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h2>The selection of silica HPLC columns<\/h2><\/div><\/div><\/div><div class=\"fl-module fl-module-rich-text fl-node-5e1d113847353\" data-node=\"5e1d113847353\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><p>Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size measurements.\u00a0The use of normal-phase HPLC has not been limited by the silica dissolution or peak tailing problems associated with reversed-phase HPLC.<\/p><p><strong>1. Particle Size<\/strong><\/p><p><strong>2. Pore Size<\/strong><\/p><p><strong>3. Other Physical Characteristics<\/strong><\/p><h2>Tama\u00f1o de las part\u00edculas<\/h2><p>For analytical work, as the quality and reproducibility of porous silica improve, the use of 3, 5 or 10\u03bcm particle size materials increases. 10\u03bcm particles are less commonly used but remain a key particle size for preparative applications. For economic reasons, irregular silicas still use a lot in industrial purification.<\/p><h2><strong>Tama\u00f1o de poro<\/strong><\/h2><p>The common pore size for porous silica is 100A, 120A, 300A. Some suppliers also have silica with 80A, 200A, or even 450A. The selection of the pore size is based on the size of the target samples. Traditional analysis for small molecules uses 100A\/120A pole size. Analysis for big molecules uses 300A. For particular samples, the choice of pores in the silica is according to the molecular size.<\/p><h2>Other Physical Characteristics<\/h2><p>The physical characteristics of the newer silica particles have been improved in several ways. However, many of them are not readily available as they are principally used as a base material for the manufacture of new reversed-phase silicas.<\/p><ul><li>Surface Activity<\/li><\/ul><p>A lower level of the unwanted, free and isolated silanol groups is observed. The lower metal ion contaminant level partly contributes to this drop in surface activity. Basic compounds interact less strongly with the silica surface resulting in improved chromatography.<\/p><ul><li>Physical Properties<\/li><\/ul><p>Improved control of physical properties such as surface area, pore-volume, mean pore diameter, and particle size has given the new silicas better lot-to-lot reproducibility.<\/p><ul><li>Purity<\/li><\/ul><p>The level of metal ion impurities has, in some cases, been reduced to cumulative figures &lt; 10ppm. Undesirable chelation of metal ion and solute has been minimised.<\/p><\/div><\/div><\/div><\/div><\/div><div class=\"fl-col fl-node-60dab52fd22c6 fl-col-bg-color fl-col-small fl-col-has-cols\" data-node=\"60dab52fd22c6\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-col-group fl-node-60dab53776a78 fl-col-group-nested\" data-node=\"60dab53776a78\"><div class=\"fl-col fl-node-5e1d11255fb0b fl-col-bg-color\" data-node=\"5e1d11255fb0b\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-sidebar fl-node-5e1d11255fa16\" data-node=\"5e1d11255fa16\"><div class=\"fl-module-content fl-node-content\"><aside id=\"block-26\" class=\"widget widget_block widget_search\"><form class=\"wp-block-search__no-button wp-block-search\" role=\"search\" action=\"https:\/\/galaklc.com\/\" method=\"get\"><label class=\"wp-block-search__label screen-reader-text\" for=\"wp-block-search__input-1\">Search<\/label><\/form><\/aside><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-842b315 e-flex e-con-boxed e-con e-parent\" data-id=\"842b315\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f00362d elementor-widget elementor-widget-text-editor\" data-id=\"f00362d\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Biovanix Silica Packing Material<\/h2><table><tbody><tr><td width=\"151\"><strong>Tama\u00f1o de las part\u00edculas<\/strong><\/td><td width=\"115\"><strong>Tama\u00f1o de poro<\/strong><\/td><td width=\"145\"><strong>Superficie<\/strong><\/td><td width=\"130\"><strong>Rango de pH<\/strong><\/td><\/tr><tr><td width=\"151\">3\/5\/10\/15\/20\/ 50\u03bcm<\/td><td width=\"115\">100\u00c5, 300\u00c5<\/td><td width=\"145\">330m<sup>2<\/sup>\/g, 200m<sup>2<\/sup>\/g<\/td><td width=\"130\">2-8<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-76387c9d e-flex e-con-boxed e-con e-parent\" data-id=\"76387c9d\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-471ea402 e-con-full e-flex e-con e-child\" data-id=\"471ea402\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1abb2595 elementor-widget elementor-widget-text-editor\" data-id=\"1abb2595\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">\u00bfTiene alg\u00fan problema con la cromatograf\u00eda l\u00edquida? P\u00f3ngase directamente en contacto con nosotros.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-10e77a30 e-con-full e-flex e-con e-child\" data-id=\"10e77a30\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-31e46092 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"31e46092\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">P\u00f3ngase en contacto con nosotros<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>How to choose proper silica HPLC column? The selection of silica HPLC columns Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"left-sidebar","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1,49],"tags":[],"class_list":["post-3797","post","type-post","status-publish","format-standard","hentry","category-blog","category-hplc-introduction"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/es\/author\/biovanix-com\/"},"uagb_comment_info":28,"uagb_excerpt":"How to choose proper silica HPLC column? The selection of silica HPLC columns Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"How to choose proper silica HPLC column? The selection of silica HPLC columnsDespite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size measurements. 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