{"id":3816,"date":"2025-01-20T14:48:10","date_gmt":"2025-01-20T14:48:10","guid":{"rendered":"https:\/\/biovanix.com\/?p=3816"},"modified":"2025-01-20T15:37:42","modified_gmt":"2025-01-20T15:37:42","slug":"ion-exchange-phases-for-liquid-chromatography-selectivity","status":"publish","type":"post","link":"https:\/\/biovanix.com\/es\/ion-exchange-phases-for-liquid-chromatography-selectivity\/","title":{"rendered":"Fases de intercambio i\u00f3nico para la selectividad en cromatograf\u00eda l\u00edquida"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"3816\" class=\"elementor elementor-3816\" data-elementor-post-type=\"post\">\n\t\t\t\t<div class=\"elementor-element elementor-element-47a326f e-flex e-con-boxed e-con e-parent\" data-id=\"47a326f\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-83fce0b elementor-widget elementor-widget-text-editor\" data-id=\"83fce0b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1><span style=\"color: #33615a;\">Fases de intercambio i\u00f3nico para la selectividad en cromatograf\u00eda l\u00edquida<\/span><\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-88504d8 e-flex e-con-boxed e-con e-parent\" data-id=\"88504d8\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b71b9c2 elementor-widget elementor-widget-text-editor\" data-id=\"b71b9c2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Ion-exchange phases separate solutes on the basis of differences in ionic charge. Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. Column efficiencies are lower than in reversed-phase HPLC. Eluents are normally aqueous but can contain some organic components.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0a90892 e-flex e-con-boxed e-con e-parent\" data-id=\"0a90892\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-084cc41 elementor-widget elementor-widget-text-editor\" data-id=\"084cc41\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Base Material<\/h2><p>Both silica-based and polymer-based ion-exchangers are available. For the former, ionic species are attached to the silica surface, whereas for the latter the ion-exchange groups are distributed throughout the matrix. Silica-based materials maintain mechanical strength and higher efficiency advantage, whereas the polymer-based materials have greater pH stability.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-09d71c1 e-flex e-con-boxed e-con e-parent\" data-id=\"09d71c1\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-33b4d92 elementor-widget elementor-widget-text-editor\" data-id=\"33b4d92\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Aplicaciones<\/h2><p>Ion-exchange is used for the analysis of small ions but the key application area of the technique is the separation of biomolecules such as peptides, proteins, and oligonucleotides. Weak ion-exchangers are used for the analysis of inorganic ions, a technique more specifically termed ion chromatography.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-80efa03 e-flex e-con-boxed e-con e-parent\" data-id=\"80efa03\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1c31fd2 elementor-widget elementor-widget-text-editor\" data-id=\"1c31fd2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Ion-Exchange Capacity<\/h2><p>The exchange capacity of an ion-exchanger is an important measure of its retentivity (typically measured in milliequivalents per gram material). For any column, the packing density of the phase must also be taken into account. Wide pore materials will typically have lower ion-exchange capacities.<\/p><p>Cation-exchange phases contain negatively charged functional groups and retain positively charged cations. Conversely, anion-exchange phases retain negatively charged analytes by their positively charged functional groups. In the schematics below, the ion strength of the counterions can be adjusted to shift the equilibrium position and thus the retention times of the analytes.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d9bcdda e-flex e-con-boxed e-con e-parent\" data-id=\"d9bcdda\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e8d57b2 elementor-widget elementor-widget-text-editor\" data-id=\"e8d57b2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Classification<\/h2><table><tbody><tr><td width=\"92\"><strong>Tipo<\/strong><\/td><td width=\"92\"><strong>Strength<\/strong><\/td><td width=\"105\"><strong>Nomenclature<\/strong><\/td><td width=\"96\"><strong>Typical Functionality<\/strong><\/td><td width=\"92\"><strong>pH Ionisation Range<\/strong><\/td><\/tr><tr><td rowspan=\"2\" width=\"92\"><strong>Anion<\/strong><\/td><td width=\"92\">D\u00e9bil<\/td><td width=\"105\">WAX<\/td><td width=\"96\">Amine<\/td><td width=\"92\">Ionised at specific pH<\/td><\/tr><tr><td width=\"92\">Fuerte<\/td><td width=\"105\">SAX<\/td><td width=\"96\">Amonio cuaternario<\/td><td rowspan=\"2\" width=\"92\">Ionised over complete pH range<\/td><\/tr><tr><td rowspan=\"2\" width=\"92\"><strong>Cation<\/strong><p>\u00a0<\/p><p><strong>\u00a0<\/strong><\/p><\/td><td width=\"92\">Fuerte<\/td><td width=\"105\">SCX<\/td><td width=\"96\">Sulphonic Acid<\/td><\/tr><tr><td width=\"92\">D\u00e9bil<\/td><td width=\"105\">WCX<\/td><td width=\"96\">\u00c1cido carbox\u00edlico<\/td><td width=\"92\">Ionised at specific pH<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-109de0b e-flex e-con-boxed e-con e-parent\" data-id=\"109de0b\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8e83ea9 elementor-widget elementor-widget-text-editor\" data-id=\"8e83ea9\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><a title=\"Ion-exchange Agarose Chromatography Media\" href=\"https:\/\/biovanix.com\/es\/ion-exchange-agarose-chromatography\/\">Biovanix Ion-exchange Phases<\/a><\/h2><table><tbody><tr><td width=\"87\"><strong>Phase<\/strong><\/td><td width=\"100\"><strong>Base Material<\/strong><\/td><td width=\"100\"><strong>Classification<\/strong><\/td><td width=\"81\"><strong>Tama\u00f1o de las part\u00edculas<\/strong><\/td><td width=\"100\"><strong>Capacidad<\/strong><\/td><td width=\"56\"><strong>Rango de pH<\/strong><\/td><\/tr><tr><td width=\"87\">PMMA SP50<\/td><td rowspan=\"6\" width=\"100\">Polymer<\/td><td width=\"100\">SCX<\/td><td width=\"81\">50um<\/td><td width=\"100\">\u2265130 mg Lysozyme<\/td><td width=\"56\">2-12<\/td><\/tr><tr><td width=\"87\">PMMA Q50<\/td><td width=\"100\">SAX<\/td><td width=\"81\">50um<\/td><td width=\"100\">\u2265130 mg Lysozyme<\/td><td width=\"56\">2-12<\/td><\/tr><tr><td width=\"87\">PSDVB POLY S50<\/td><td width=\"100\">SCX<\/td><td width=\"81\">50\u03bcm<\/td><td width=\"100\">\u2265 60 mg\/ml hlgG<\/td><td width=\"56\">2-12<\/td><\/tr><tr><td width=\"87\">PSDVB POLY CM50<\/td><td width=\"100\">WCX<\/td><td width=\"81\">50\u03bcm<\/td><td width=\"100\">\u226580 mg Lysozyme<\/td><td width=\"56\">6-12<\/td><\/tr><tr><td width=\"87\">PSDVB POLY Q50<\/td><td width=\"100\">SAX<\/td><td width=\"81\">50\u03bcm<\/td><td width=\"100\">\u2265 100 mg BSA<\/td><td width=\"56\">2-12<\/td><\/tr><tr><td width=\"87\">PSDVB POLY D50<\/td><td width=\"100\">WAX<\/td><td width=\"81\">50\u03bcm<\/td><td width=\"100\">\u2265 100 mg BSA<\/td><td width=\"56\">2-9<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6b15b980 e-flex e-con-boxed e-con e-parent\" data-id=\"6b15b980\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-31ac98a4 e-con-full e-flex e-con e-child\" data-id=\"31ac98a4\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5748249f elementor-widget elementor-widget-text-editor\" data-id=\"5748249f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">\u00bfTiene alg\u00fan problema con la cromatograf\u00eda l\u00edquida? P\u00f3ngase directamente en contacto con nosotros.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-51361e47 e-con-full e-flex e-con e-child\" data-id=\"51361e47\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-419ed1a1 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"419ed1a1\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">P\u00f3ngase en contacto con nosotros<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Ion-exchange Phases For Liquid Chromatography Selectivity Ion-exchange phases separate solutes on the basis of differences in ionic charge. Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. Column efficiencies are lower than in reversed-phase HPLC. Eluents are normally aqueous but can contain [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"left-sidebar","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3816","post","type-post","status-publish","format-standard","hentry","category-blog"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/es\/author\/biovanix-com\/"},"uagb_comment_info":29,"uagb_excerpt":"Ion-exchange Phases For Liquid Chromatography Selectivity Ion-exchange phases separate solutes on the basis of differences in ionic charge. Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. Column efficiencies are lower than in reversed-phase HPLC. Eluents are normally aqueous but can contain&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Ion-exchange Phases For Liquid Chromatography Selectivity Ion-exchange phases separate solutes on the basis of differences in ionic charge. Retention in ion-exchange chromatography is determined by the pH of the eluent, the nature and ionic strength of the buffer and temperature. Column efficiencies are lower than in reversed-phase HPLC. 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