{"id":3212,"date":"2024-11-04T02:32:05","date_gmt":"2024-11-04T02:32:05","guid":{"rendered":"https:\/\/biovanix.com\/?post_type=product&#038;p=3212"},"modified":"2025-03-10T15:42:59","modified_gmt":"2025-03-10T15:42:59","slug":"ni-ted-6ff-agarose-chromatography-media","status":"publish","type":"product","link":"https:\/\/biovanix.com\/es\/product\/ni-ted-6ff-agarose-chromatography-media\/","title":{"rendered":"Medio de cromatograf\u00eda de agarosa Ni-TED 6FF"},"content":{"rendered":"<h1>Medio de cromatograf\u00eda de agarosa Ni-TED 6FF<\/h1>\n<p>Los medios de Agarosa Biovanix se han dise\u00f1ado bas\u00e1ndose en la serie Cytive Sepharose. Ofrece una alta especificidad y selectividad para separaciones y purificaciones biomoleculares. La separaci\u00f3n por afinidad a menudo puede eliminar contaminantes dif\u00edciles de eliminar mediante otros procedimientos cromatogr\u00e1ficos. Se pueden conseguir purificaciones de varios \u00f3rdenes de magnitud en un solo paso.<\/p>\n<ul>\n<li>Medio de cromatograf\u00eda de afinidad quelante de Ni2+ en agarosa;<\/li>\n<li>Alta capacidad de adsorci\u00f3n din\u00e1mica y funcionamiento de caudal r\u00e1pido;<\/li>\n<li>Se utiliza principalmente para la separaci\u00f3n y purificaci\u00f3n de prote\u00ednas de ingenier\u00eda gen\u00e9tica marcadas con histidina.<\/li>\n<\/ul>\n<h3>Par\u00e1metro<\/h3>\n<table width=\"405\">\n<tbody>\n<tr>\n<td width=\"183\"><\/td>\n<td width=\"215\"><strong>Ni-TED 6FF<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Matriz<\/strong><\/td>\n<td width=\"215\">Agarosa reticulada 6%<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Tama\u00f1o medio de las part\u00edculas<\/strong><\/td>\n<td width=\"215\">90\u03bcm<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Grupo modificado<\/strong><\/td>\n<td width=\"215\">-NTA Ni<sup>2+<\/sup><\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Encuadernaci\u00f3n din\u00e1mica<\/strong><\/p>\n<p><strong>Capacidad<\/strong><\/td>\n<td width=\"215\">25 mg His\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Concentraci\u00f3n del ligando<\/strong><\/td>\n<td width=\"215\">90-130 \u03bcmol\/mL resin<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>pH<\/strong> <strong>Estabilidad, funcionamiento<\/strong><\/td>\n<td width=\"215\">2-12<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>pH<\/strong> <strong>Estabilidad, CIP<\/strong><\/td>\n<td width=\"215\">2-14<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Presi\u00f3n<\/strong><\/td>\n<td width=\"215\">\u22640,3MPa<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Temperatura, funcionamiento<\/strong><\/td>\n<td width=\"215\">4-40\u2103<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Termoestabilidad<\/strong><\/td>\n<td width=\"215\">120\u2103\uff0c30min, pH 7<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Caudal<\/strong><\/td>\n<td width=\"215\">600 cm\/h<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Estabilidad qu\u00edmica<\/strong><\/td>\n<td width=\"215\">Tamp\u00f3n acuoso, NaOH 0,01M, HCl 0,01M (1 semana); EDTA 10 mM, DTT 5 mM, TCEP 5 mM, \u03b2-mercaptoetanol 20 mM, NaOH 1 M, clorhidrato de guanidina 6 M (24 horas); imidazol 500 mM, EDTA 100 mM (2 horas); alcohol isoprop\u00edlico 30% (20 minutos).<\/td>\n<\/tr>\n<tr>\n<td width=\"183\"><strong>Almacenamiento<\/strong><\/td>\n<td width=\"215\">20% EtOH, 4-30\u2103<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: 1.5em; font-weight: bold;\">Paquete<\/span><\/p>\n<table width=\"635\">\n<tbody>\n<tr>\n<td width=\"263\"><b>\u00a0<\/b><\/td>\n<td width=\"372\"><b>Paquete<\/b><\/td>\n<\/tr>\n<tr>\n<td rowspan=\"6\" width=\"263\"><b>Paquete peque\u00f1o<\/b><\/td>\n<td width=\"372\">25 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">50 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">100 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">200 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">500 ml<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">1L<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"2\" width=\"263\">Paquete de producci\u00f3n<\/td>\n<td width=\"372\">10L<\/td>\n<\/tr>\n<tr>\n<td width=\"372\">25L<\/td>\n<\/tr>\n<\/tbody>\n<\/table>","protected":false},"excerpt":{"rendered":"<p>Ni-TED 6FF Agarose Chromatography Media Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step. 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6FF Agarose Chromatography Media Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step.&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"BioVanix Ni-TED 6FF Agarose Chromatography Media is based on high-quality agarose particles with Ni IDA ligand. Factory Supply. 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