{"id":2186,"date":"2024-05-17T03:27:26","date_gmt":"2024-05-17T03:27:26","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=2186"},"modified":"2025-03-04T15:18:20","modified_gmt":"2025-03-04T15:18:20","slug":"colonne-hplc-daffinite-pour-la-proteine-a-pour-lanalyse-mab","status":"publish","type":"page","link":"https:\/\/biovanix.com\/fr\/colonne-hplc-daffinite-pour-la-proteine-a-pour-lanalyse-mab\/","title":{"rendered":"Colonne HPLC d'affinit\u00e9 pour la prot\u00e9ine A pour l'analyse des AcM"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2186\" class=\"elementor elementor-2186\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3e866b3 e-flex e-con-boxed e-con e-parent\" data-id=\"3e866b3\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-53c97f5 e-con-full e-flex e-con e-child\" data-id=\"53c97f5\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-993e5dd elementor-widget elementor-widget-image\" data-id=\"993e5dd\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2190\" alt=\"Colonne HPLC Biovanix Protein A\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-100x100.jpg 100w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column.jpg 500w\" sizes=\"(max-width: 300px) 100vw, 300px\" title=\"Biovanix Protein A HPLC Column | BioVanix\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d6e4b77 e-con-full e-flex e-con e-child\" data-id=\"d6e4b77\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e959cf2 elementor-widget elementor-widget-heading\" data-id=\"e959cf2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Colonne CLHP d'affinit\u00e9 pour la prot\u00e9ine A Prosep<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b4ed5e1 elementor-widget elementor-widget-heading\" data-id=\"b4ed5e1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Pour l'analyse HPLC des AcM<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-61a1c91 elementor-widget elementor-widget-text-editor\" data-id=\"61a1c91\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>La colonne HPLC Prosep mAb analysis est utilis\u00e9e pour l'analyse des anticorps monoclonaux en laboratoire. La d\u00e9termination de la concentration d'IgG (titre) permet le criblage rapide des \u00e9chantillons de culture cellulaire et l'optimisation des conditions de fermentation.<\/p>\n<ul>\n<li>Utilisation directe sur les instruments HPLC<\/li>\n<li>Analyse rapide et pr\u00e9cise de la concentration d'anticorps<\/li>\n<li>Large gamme de lin\u00e9arit\u00e9<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1000114 elementor-widget elementor-widget-text-editor\" data-id=\"1000114\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><span style=\"color: #ffffff;\"><a style=\"color: #ffffff;\" title=\"Rapport de test comparatif pour la colonne HPLC d&#039;affinit\u00e9 Prosep Protein A\" href=\"https:\/\/biovanix.com\/fr\/rapport-de-test-de-comparaison-pour-la-colonne-hplc-daffinite-pour-la-proteine-a-de-prosep\/\"><span style=\"text-decoration: underline;\">Rapport de test comparatif avec POR*S Une colonne du fabricant chinois TOP CXO, cliquez ici &gt;&gt;&gt;<\/span><\/a><\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-0086744 e-flex e-con-boxed e-con e-parent\" data-id=\"0086744\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c3dccd6 elementor-widget elementor-widget-text-editor\" data-id=\"c3dccd6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><strong>Param\u00e8tres techniques<\/strong><\/h2>\n<table>\n<tbody>\n<tr>\n<td>\n<p><strong>\u00a0<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Ligand<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Substrat<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Pression<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Gamme de pH<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Capacit\u00e9 dynamique<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>D\u00e9bit<\/strong><\/p>\n<\/td>\n<td>\n<p><strong>Temps de vie<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p>Colonne Prosep Protein A PLUS<\/p>\n<\/td>\n<td>\n<p>rProt\u00e9ine A<\/p>\n<\/td>\n<td>\n<p>20 \u03bcm PSDVB<\/p>\n<\/td>\n<td>\n<p>1200psi<\/p>\n<\/td>\n<td>\n<p>2-11<\/p>\n<\/td>\n<td>\n<p>40 mg d'IgG humaine\/ml de milieu[1]<\/p>\n<\/td>\n<td>\n<p>1-3ml\/min<\/p>\n<\/td>\n<td>\n<p>1000-3000 cercles<span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-weight: var( --e-global-typography-text-font-weight ); text-align: var(--text-align); background-color: var(--ast-global-color-5); font-size: 1rem;\">[2]<\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td>\n<p>Colonne Prosep Protein A<\/p>\n<\/td>\n<td>\n<p>rProt\u00e9ine A<\/p>\n<\/td>\n<td>\n<p>40 \u03bcm PSDVB<\/p>\n<\/td>\n<td>\n<p>1200psi<\/p>\n<\/td>\n<td>\n<p>2-11<\/p>\n<\/td>\n<td>\n<p>40 mg d'IgG humaine\/ml de milieu<span style=\"color: var( --e-global-color-text ); font-family: var( --e-global-typography-text-font-family ), Sans-serif; font-weight: var( --e-global-typography-text-font-weight ); text-align: var(--text-align); background-color: var(--ast-global-color-5); font-size: 1rem;\">[1]<\/span><\/p>\n<\/td>\n<td>\n<p>1-3ml\/min<\/p>\n<\/td>\n<td>\n<p>1000-3000 cercles[2]<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<ol>\n<li>PS\/DVB : Poly(styr\u00e8ne\/divinylbenz\u00e8ne)\u00a0<\/li>\n<li>Les r\u00e9sultats des tests sont bas\u00e9s sur des tests de laboratoire locaux.<\/li>\n<\/ol>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-39d9182 e-flex e-con-boxed e-con e-parent\" data-id=\"39d9182\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-42979f0 e-con-full e-flex e-con e-child\" data-id=\"42979f0\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ed4ce2c elementor-widget elementor-widget-text-editor\" data-id=\"ed4ce2c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>Prosep Prot\u00e9ine A Produit<\/h2>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-36f357d e-con-full e-flex e-con e-child\" data-id=\"36f357d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-c395611 elementor-widget elementor-widget-image\" data-id=\"c395611\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/fr\/product\/protein-a-affinity-hplc-column\/\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2190\" alt=\"Colonne HPLC Biovanix Protein A\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column-100x100.jpg 100w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/05\/Biovanix-Protein-A-HPLC-Column.jpg 500w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-734e1a86 e-flex e-con-boxed e-con e-parent\" data-id=\"734e1a86\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-46c36a35 elementor-widget elementor-widget-text-editor\" data-id=\"46c36a35\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\n<h2><strong>Condition d'analyse pour la colonne Prosep Protein A<\/strong><\/h2>\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\"><strong>\u00c9luant A<\/strong><\/th>\n<th align=\"left\"><strong>20-50 mM Phosphate, 150 mM NaCl, pH7-7.5<\/strong><\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\"><strong>\u00c9luant B<\/strong><\/td>\n<td align=\"left\">12 mM HCl, 150 mM NaCl, pH1,9, ou 100 mM Gly-HCl, 150 mM NaCl, pH2,5<\/td>\n<\/tr>\n<tr>\n<th align=\"left\"><strong>D\u00e9bit<\/strong><\/th>\n<th align=\"left\"><strong>1 mL\/min<\/strong><\/th>\n<\/tr>\n<tr>\n<td align=\"left\"><strong>Injection<\/strong><\/td>\n<td align=\"left\">1-100 \u03bcL<\/td>\n<\/tr>\n<tr>\n<th align=\"left\"><strong>D\u00e9tection<\/strong><\/th>\n<th align=\"left\">UV 280nm<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><strong>M\u00e9thode Calendrier<\/strong><\/p>\n<table width=\"100%\">\n<thead>\n<tr>\n<th align=\"left\"><strong>Temps (min)<\/strong><\/th>\n<th align=\"left\"><strong>Gradient(%B)<\/strong><\/th>\n<th align=\"left\">\u00a0<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td align=\"left\">0.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">\u00c9quilibrage\/lavage, 1 min, 10 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">1.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">\u00c9quilibrage\/lavage, 1 min, 10 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">1.01<\/td>\n<td align=\"left\">100<\/td>\n<td align=\"left\">Elution, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">3.00<\/td>\n<td align=\"left\">100<\/td>\n<td align=\"left\">Elution, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">3.01<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">R\u00e9\u00e9quilibrage, 2 min, 20 CV<\/td>\n<\/tr>\n<tr>\n<td align=\"left\">5.00<\/td>\n<td align=\"left\">0<\/td>\n<td align=\"left\">R\u00e9\u00e9quilibrage, 2 min, 20 CV<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>* Les conditions d'analyse varient selon les machines.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-71a0e466 e-flex e-con-boxed e-con e-parent\" data-id=\"71a0e466\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-650a23d1 e-con-full e-flex e-con e-child\" data-id=\"650a23d1\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-71fb0b4 elementor-widget elementor-widget-text-editor\" data-id=\"71fb0b4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">Vous ne trouvez pas le produit dont vous avez besoin ? Contactez nous directement.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-1760f951 e-con-full e-flex e-con e-child\" data-id=\"1760f951\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-112a86e7 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"112a86e7\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Nous contacter<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Prosep Protein A Affinity HPLC Column For mAb HPLC Analysis Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Direct use on HPLC instrument Fast and accurate analysis of antibody concentration [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-2186","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/fr\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"Prosep Protein A Affinity HPLC Column For mAb HPLC Analysis Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Direct use on HPLC instrument Fast and accurate analysis of antibody concentration\u2026","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Biovanix Protein A affinity HPLC column is for mAb analysis. Stainless steel and PEEK columns with 2.1-30 &amp; 4.6-50mm. 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