{"id":2339,"date":"2024-08-18T14:02:44","date_gmt":"2024-08-18T14:02:44","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=2339"},"modified":"2025-11-20T18:11:14","modified_gmt":"2025-11-20T10:11:14","slug":"milieux-de-chromatographie-sur-agarose","status":"publish","type":"page","link":"https:\/\/biovanix.com\/fr\/agarose-chromatography-media\/","title":{"rendered":"Milieu de chromatographie en agarose | BioVanix"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"2339\" class=\"elementor elementor-2339\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1852585 e-con-full e-flex e-con e-parent\" data-id=\"1852585\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t<div class=\"elementor-element elementor-element-efb9092 elementor-widget elementor-widget-text-editor\" data-id=\"efb9092\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1><span style=\"color: #ffffff;\">Milieux de chromatographie en agarose<\/span><\/h1><p><span style=\"color: #ffffff;\">Biovanix Agarose Media est un milieu de s\u00e9paration biologique conventionnel, fabriqu\u00e9 \u00e0 partir de microsph\u00e8res d'agarose hautement r\u00e9ticul\u00e9es. Il utilise des mati\u00e8res premi\u00e8res d'agarose de haute performance, import\u00e9es, pour maintenir un niveau de qualit\u00e9 de produit constant. Ce produit conserve l'hydrophilie remarquable et la structure r\u00e9ticulaire \u00e9tendue inh\u00e9rentes aux polysaccharides naturels, d\u00e9montrant une excellente affinit\u00e9 pour les macromol\u00e9cules bioactives. Il se caract\u00e9rise par une haute capacit\u00e9 de chargement, une large applicabilit\u00e9, une adsorption non sp\u00e9cifique et une dynamique de flux rapide. Par cons\u00e9quent, il est largement utilis\u00e9 dans la pr\u00e9paration \u00e0 l'\u00e9chelle du laboratoire de macromol\u00e9cules biologiques, notamment les prot\u00e9ines, les acides nucl\u00e9iques, les peptides et les polysaccharides, ainsi que dans la production industrielle \u00e0 grande \u00e9chelle de produits biopharmaceutiques et les applications de bioing\u00e9nierie.<\/span><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-37fc69b e-flex e-con-boxed e-con e-parent\" data-id=\"37fc69b\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-cd20c01 elementor-widget elementor-widget-text-editor\" data-id=\"cd20c01\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3>Caract\u00e9ristiques des milieux de chromatographie sur agarose<\/h3><p data-path-to-node=\"3\">Les milieux \u00e0 base d'agarose repr\u00e9sentent la r\u00e9f\u00e9rence en mati\u00e8re de matrice dans le domaine de la biopurification. D\u00e9riv\u00e9es de polysaccharides naturels et optimis\u00e9es par des technologies de r\u00e9ticulation modernes, ces matrices pr\u00e9sentent une combinaison unique de propri\u00e9t\u00e9s physico-chimiques id\u00e9ales pour la purification de prot\u00e9ines, d'anticorps et d'autres biomacromol\u00e9cules.<\/p><p data-path-to-node=\"4\">Voici les principales caract\u00e9ristiques techniques :<\/p><h4>1. Hydrophilie exceptionnelle et bio-inertie<\/h4><p data-path-to-node=\"6\">L'avantage fondamental de l'agarose est sa nature non toxique et hydrophile.<\/p><ul data-path-to-node=\"7\"><li><p data-path-to-node=\"7,0,0\"><b>Faible liaison non sp\u00e9cifique (NSB) :<\/b> Le squelette d'agarose est riche en groupes hydroxyles, cr\u00e9ant une surface qui interagit favorablement avec l'eau mais pr\u00e9sente une interaction hydrophobe minimale avec les prot\u00e9ines. Cela r\u00e9duit consid\u00e9rablement l'adsorption non sp\u00e9cifique, garantissant des taux de r\u00e9cup\u00e9ration \u00e9lev\u00e9s et emp\u00eachant la d\u00e9naturation des cibles biologiques sensibles.<\/p><\/li><li><p data-path-to-node=\"7,1,0\"><b>Biocompatibilit\u00e9 :<\/b> L'environnement inerte pr\u00e9serve la structure tertiaire et l'activit\u00e9 biologique des enzymes, des anticorps et d'autres prot\u00e9ines complexes pendant le processus de s\u00e9paration.<\/p><\/li><\/ul><h4>2. Structure r\u00e9ticulaire macroporeuse<\/h4><p data-path-to-node=\"9\">L'agarose forme un r\u00e9seau hydrogel tridimensionnel caract\u00e9ris\u00e9 par de grands pores ouverts.<\/p><ul data-path-to-node=\"10\"><li><p data-path-to-node=\"10,0,0\"><b>Transfert de Masse \u00c9lev\u00e9 :<\/b> La structure macroporeuse (g\u00e9n\u00e9ralement ajust\u00e9e en fonction de la concentration en agarose, par exemple 4% ou 6%) permet une diffusion rapide des macromol\u00e9cules vers l&#x27;int\u00e9rieur et l&#x27;ext\u00e9rieur des billes. Ceci est essentiel pour maintenir une haute r\u00e9solution et une capacit\u00e9 de liaison dynamique (DBC), m\u00eame \u00e0 des d\u00e9bits \u00e9lev\u00e9s.<\/p><\/li><li><p data-path-to-node=\"10,1,0\"><b>Accessibilit\u00e9 :<\/b> Contrairement \u00e0 la silice ou aux polym\u00e8res \u00e0 pores plus petits, l'agarose est particuli\u00e8rement adapt\u00e9e \u00e0 la s\u00e9paration de biomol\u00e9cules de grande taille, telles que les immunoglobulines (IgG), les particules de type viral (VLP) et les plasmides, car ces mol\u00e9cules peuvent facilement acc\u00e9der \u00e0 la surface int\u00e9rieure des billes.<\/p><\/li><\/ul><h4>3. Chimie de Surface Polyvalente pour le Couplage de Ligands<\/h4><p data-path-to-node=\"12\">L'agarose est chimiquement polyvalent et facilement activable, ce qui en fait la matrice de choix pour la chromatographie d'affinit\u00e9 et d'\u00e9change d'ions.<\/p><ul data-path-to-node=\"13\"><li><p data-path-to-node=\"13,0,0\"><b>Facilit\u00e9 de d\u00e9rivatisation<\/b> Les groupes hydroxyles primaires sur les r\u00e9sidus de sucre servent de points d'attache pr\u00eats pour diverses m\u00e9thodes d'activation chimique (par exemple, bromure de cyanog\u00e8ne, ester de NHS, \u00e9pichlorhydrine).<\/p><\/li><li><p data-path-to-node=\"13,1,0\"><b>Stabilit\u00e9 du ligand<\/b> Il supporte la fixation multipoint de ligands (tels que la Prot\u00e9ine A, la Prot\u00e9ine G, ou des groupes fonctionnels charg\u00e9s) sans compromettre la stabilit\u00e9 de la matrice ni l'activit\u00e9 du ligand.<\/p><\/li><\/ul><h4>4. Stabilit\u00e9 m\u00e9canique am\u00e9lior\u00e9e (r\u00e9ticulation)<\/h4><p data-path-to-node=\"15\">Alors que l'agarose natif est m\u00e9caniquement mou, les milieux \u00e0 base d'agarose de qualit\u00e9 industrielle subissent <b>r\u00e9ticulation chimique<\/b> pour en am\u00e9liorer la rigidit\u00e9.<\/p><ul data-path-to-node=\"16\"><li><p data-path-to-node=\"16,0,0\"><b>Caract\u00e9ristiques Pression-D\u00e9bit :<\/b> L'agarose hautement r\u00e9ticul\u00e9e (souvent d\u00e9sign\u00e9e sous le nom de \u201c Fast Flow \u201d ou \u201c High Flow \u201d) peut supporter des contre-pressions plus \u00e9lev\u00e9es. Cette rigidit\u00e9 emp\u00eache la compression du lit et permet des d\u00e9bits lin\u00e9aires \u00e9lev\u00e9s, essentiels pour r\u00e9duire les temps de cycle dans la fabrication \u00e0 l'\u00e9chelle industrielle.<\/p><\/li><li><p data-path-to-node=\"16,1,0\"><b>\u00c9volutivit\u00e9 :<\/b> La r\u00e9sistance m\u00e9canique garantit que les param\u00e8tres de performance restent constants lors du passage de colonnes de laboratoire \u00e0 des colonnes de proc\u00e9d\u00e9 industriel.<\/p><\/li><\/ul><h4>5. Stabilit\u00e9 chimique robuste<\/h4><p data-path-to-node=\"18\">L'agarose r\u00e9ticul\u00e9 pr\u00e9sente une r\u00e9sistance significative \u00e0 la d\u00e9gradation chimique, ce qui facilite des protocoles de nettoyage et de d\u00e9sinfection rigoureux.<\/p><ul data-path-to-node=\"19\"><li><p data-path-to-node=\"19,0,0\"><b>R\u00e9sistance CIP :<\/b> Il tol\u00e8re l'exposition \u00e0 des agents de nettoyage en place (NEP) agressifs, notamment des concentrations \u00e9lev\u00e9es d'hydroxyde de sodium<span class=\"math-inline\" data-math=\"0.1\"><span class=\"katex\"><span class=\"katex-html\" aria-hidden=\"true\"><span class=\"base\"><span class=\"mord\">0.1<\/span><\/span><\/span><\/span><\/span> \u2013 <span class=\"math-inline\" data-math=\"1.0 \\text{ M NaOH}\"><span class=\"katex\"><span class=\"katex-html\" aria-hidden=\"true\"><span class=\"base\"><span class=\"mord\">1.0<\/span><span class=\"mord text\"><span class=\"mord\">\u00a0M NaOH<\/span><\/span><\/span><\/span><\/span><\/span>Cela permet l'\u00e9limination efficace des prot\u00e9ines pr\u00e9cipit\u00e9es, des lipides et des endotoxines, prolongeant ainsi la dur\u00e9e de vie de la r\u00e9sine.<\/p><\/li><li><p data-path-to-node=\"19,1,0\"><b>Compatibilit\u00e9 avec les solvants :<\/b> Il est stable dans les tampons aqueux sur une large gamme de pH (typiquement pH 3-14) et compatible avec de nombreux solvants organiques et agents chaotropiques (par exemple, ur\u00e9e, chlorhydrate de guanidine) utilis\u00e9s pour la r\u00e9g\u00e9n\u00e9ration de colonnes.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a889b8a e-flex e-con-boxed e-con e-parent\" data-id=\"a889b8a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7ef55f3 elementor-widget elementor-widget-text-editor\" data-id=\"7ef55f3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2 style=\"text-align: center;\"><span style=\"color: #33615a;\">Milieux de chromatographie en agarose<\/span><\/h2>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-4f302e0 e-flex e-con-boxed e-con e-parent\" data-id=\"4f302e0\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-17d757b e-con-full e-flex e-con e-child\" data-id=\"17d757b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ef7c7a0 elementor-widget elementor-widget-image\" data-id=\"ef7c7a0\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/fr\/affinity-agarose-chromatography\/\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2358\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1024x1024.jpg 1024w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-768x768.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1536x1536.jpg 1536w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-2048x2048.jpg 2048w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-600x600.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-100x100.jpg 100w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4ebc8d1 elementor-widget elementor-widget-text-editor\" data-id=\"4ebc8d1\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4 style=\"text-align: center;\"><a href=\"https:\/\/biovanix.com\/fr\/chromatographie-daffinite-en-agarose\/\">Affinity Media<\/a><\/h4>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-26fc70f e-con-full e-flex e-con e-child\" data-id=\"26fc70f\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1620954 elementor-widget elementor-widget-image\" data-id=\"1620954\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/fr\/ion-exchange-agarose-chromatography\/\" target=\"_blank\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2357\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-768x768.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-600x600.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5-100x100.jpg 100w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-5.jpg 800w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1681886 elementor-widget elementor-widget-text-editor\" data-id=\"1681886\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4 style=\"text-align: center;\">Milieu \u00e9changeur d'ions<\/h4>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-e45a33a e-con-full e-flex e-con e-child\" data-id=\"e45a33a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0a4d22d elementor-widget elementor-widget-image\" data-id=\"0a4d22d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/fr\/hydrophobic-chromatography\/\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2358\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1024x1024.jpg 1024w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-768x768.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1536x1536.jpg 1536w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-2048x2048.jpg 2048w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-600x600.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-100x100.jpg 100w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4bf3a30 elementor-widget elementor-widget-text-editor\" data-id=\"4bf3a30\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4 style=\"text-align: center;\"><a href=\"https:\/\/biovanix.com\/fr\/chromatographie-dinteraction-hydrophobe\/\">Milieux hydrophobes<\/a><\/h4>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-59bc8e3 e-con-full e-flex e-con e-child\" data-id=\"59bc8e3\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-458d5b5 elementor-widget elementor-widget-image\" data-id=\"458d5b5\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/biovanix.com\/fr\/gel-filtration-agarose-chromatography-media\/\">\n\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"300\" height=\"300\" src=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg\" class=\"attachment-medium size-medium wp-image-2358\" alt=\"\" srcset=\"https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-300x300.jpg 300w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1024x1024.jpg 1024w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-150x150.jpg 150w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-768x768.jpg 768w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-1536x1536.jpg 1536w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-2048x2048.jpg 2048w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-600x600.jpg 600w, https:\/\/biovanix.com\/wp-content\/uploads\/2024\/08\/\u74f6\u5b50-4-100x100.jpg 100w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-0517043 elementor-widget elementor-widget-text-editor\" data-id=\"0517043\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4 style=\"text-align: center;\"><a title=\"Milieu de chromatographie en agarose pour filtration sur gel\" href=\"https:\/\/biovanix.com\/fr\/milieux-de-chromatographie-en-agarose-par-filtration-sur-gel\/\">M\u00e9dia de filtration sur gel<\/a><\/h4>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9c7e04a e-flex e-con-boxed e-con e-parent\" data-id=\"9c7e04a\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-687142a elementor-widget elementor-widget-text-editor\" data-id=\"687142a\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h4><span style=\"font-size: 1.7em; font-weight: bold; color: #33615a;\">Milieux de chromatographie d'affinit\u00e9<\/span><\/h4><table width=\"639\"><tbody><tr><td width=\"136\"><p><b>Produit<\/b><\/p><\/td><td width=\"174\"><p><b>Capacit\u00e9 de liaison dynamique<\/b><\/p><\/td><td width=\"328\"><p><b>Application<\/b><\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose Ni-IDA 6FF\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-ni-ida\/\">Ni-IDA 6FF<\/a><\/p><\/td><td width=\"174\"><p>40 mg His\/mL<\/p><\/td><td rowspan=\"2\" width=\"328\"><p>Capacit\u00e9 de charge \u00e9lev\u00e9e<\/p><p>Isolement et purification de prot\u00e9ines recombinantes marqu\u00e9es \u00e0 l'histidine (His-Tag)<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose Ni-IDA 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/ni-ida-6hp-agarose-chromatography-media-copy\/\">Ni-IDA 6HP<\/a><\/p><\/td><td width=\"174\"><p>40 mg His\/mL<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose Ni-NTA 6FF\" href=\"https:\/\/biovanix.com\/fr\/product\/ni-ntf-6ff-support-de-chromatographie-sur-agarose\/\">Ni-NTA 6FF<\/a><\/p><\/td><td width=\"174\"><p>50 mg His\/mL<\/p><\/td><td rowspan=\"2\" width=\"328\"><p>Faible Ni<sup>2+<\/sup> fuite<\/p><p>Isolement et purification de prot\u00e9ines recombinantes marqu\u00e9es \u00e0 l'histidine (His-Tag)<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose Ni-NTA 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/ni-nta-6hp-agarose-chromatography-media\/\">Ni-NTA 6HP<\/a><\/p><\/td><td width=\"174\"><p>50 mg His\/mL<\/p><\/td><\/tr><tr><td width=\"136\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/ni-ted-6ff-agarose-chromatography-media\/\">Ni-TED 6FF<\/a><\/p><\/td><td width=\"174\"><p>25 mg His\/mL<\/p><\/td><td rowspan=\"2\" width=\"328\"><p>Principalement utilis\u00e9 pour la s\u00e9paration et la purification des prot\u00e9ines du g\u00e9nie g\u00e9n\u00e9tique marqu\u00e9es \u00e0 l'histidine (His-Tag) contenant de l'EDTA ou du DTT et d'autres composants.<\/p><\/td><\/tr><tr><td width=\"136\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/ni-ted-6hp-agarose-chromatography-media\/\">Ni-TED 6HP<\/a><\/p><\/td><td width=\"174\"><p>25 mg His\/mL<\/p><\/td><\/tr><tr><td width=\"136\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/protein-g-agarose-chromatography-media\/\">Prot\u00e9ine G 4FF<\/a><\/p><\/td><td width=\"174\"><p>35 mg d'IgG\/mL<\/p><\/td><td width=\"328\"><p>Purification par affinit\u00e9 de divers anticorps polyclonaux et monoclonaux<\/p><\/td><\/tr><tr><td width=\"136\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/protein-a-g-agarose-chromatography-media\/\">Prot\u00e9ine A 4FF<\/a><\/p><\/td><td width=\"174\"><p>50 mg d'IgG\/mL<\/p><\/td><td width=\"328\"><p>R\u00e9sistance alcaline, \u00e9lution facile<\/p><p>Purification par affinit\u00e9 de divers anticorps polyclonaux et monoclonaux<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose GSH 4FF\" href=\"https:\/\/biovanix.com\/fr\/product\/gsh-4ff-agarose-chromatography-media\/\">GSH 4FF<\/a><\/p><\/td><td width=\"174\"><p>10 mg GST\/mL<\/p><\/td><td width=\"328\"><p>Isolement et purification de la prot\u00e9ine marqu\u00e9e par la glutathion transf\u00e9rase (prot\u00e9ine de fusion GST), de la glutathion transf\u00e9rase et de la prot\u00e9ine d\u00e9pendante du glutathion<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose h\u00e9parine 6FF\" href=\"https:\/\/biovanix.com\/fr\/product\/heparin-6ff-agarose-chromatography-media\/\">H\u00e9parine 6FF<\/a><\/p><\/td><td width=\"174\"><p>1,5 mg AT \u2162\/mL<\/p><\/td><td rowspan=\"2\" width=\"328\"><p>Isolement et purification de l'AT \u2162, du facteur de coagulation, de la lipoprot\u00e9ine, de la lipase et du polysaccharide<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose h\u00e9parine 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-heparine-6hp\/\">H\u00e9parine 6HP<\/a><\/p><\/td><td width=\"174\"><p>1,5 mg AT \u2162\/mL<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"Milieu de chromatographie en agarose Benzamidine 4FF\" href=\"https:\/\/biovanix.com\/fr\/product\/benzamidine-4ff-agarose-chromatography-media\/\">Benzamidine 4FF<\/a><\/p><\/td><td width=\"174\"><p>20 mg Trypsine\/mL (High Sub)<\/p><p>10 mg Trypsine\/mL (Low Sub)<\/p><\/td><td width=\"328\"><p>Isolement et purification de la trypsine, de la thrombine, de l'urokinase, de la kallikr\u00e9ine, de la pr\u00e9kallikr\u00e9ine et d'autres prot\u00e9ases \u00e0 s\u00e9rine<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"MMA 6FF Milieu de chromatographie en agarose\" href=\"https:\/\/biovanix.com\/fr\/product\/mma-6ff-milieu-de-chromatographie-en-agarose\/\">MMA 6FF<\/a><\/p><\/td><td width=\"174\"><p>25 mg BSA\/mL<\/p><\/td><td width=\"328\"><p>Largement utilis\u00e9 pour la s\u00e9paration et la purification des prot\u00e9ines, en particulier pour l'\u00e9limination de la prot\u00e9ine A des anticorps monoclonaux qui ont \u00e9t\u00e9 \u00e9limin\u00e9s par le milieu d'affinit\u00e9 de la prot\u00e9ine A, ainsi que des dim\u00e8res d'anticorps, des prot\u00e9ines de l'h\u00f4te, des acides nucl\u00e9iques et des virus.<\/p><\/td><\/tr><tr><td width=\"136\"><p><a title=\"MMC 6FF Milieu de chromatographie en agarose\" href=\"https:\/\/biovanix.com\/fr\/product\/mmc-6ff-agarose-chromatography-media\/\">MMC 6FF<\/a><\/p><\/td><td width=\"174\"><p>60 mg BSA\/mL<\/p><\/td><td width=\"328\"><p>Largement utilis\u00e9 dans la s\u00e9paration et la purification des prot\u00e9ines<\/p><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b573b3d elementor-widget elementor-widget-text-editor\" data-id=\"b573b3d\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<table width=\"100%\"><tbody><tr><td width=\"38%\"><p><strong>Produit<\/strong><\/p><\/td><td width=\"29%\"><p><strong>Capacit\u00e9 de liaison dynamique<\/strong><\/p><\/td><td width=\"29%\"><p><strong>Application<\/strong><\/p><\/td><\/tr><tr><td width=\"38%\"><p>Prosep MMA<\/p><\/td><td width=\"29%\"><p>20 mg BSA\/mL<\/p><\/td><td rowspan=\"6\" width=\"29%\"><p>Grande rigidit\u00e9<\/p><p>D\u00e9bit \u00e9lev\u00e9<\/p><p>Haute r\u00e9solution<\/p><p>Chargement rapide<\/p><\/td><\/tr><tr><td width=\"38%\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/mma-hpr-agarose-chromatography-media\/\">Prosep MMA HPR<\/a><\/p><\/td><td width=\"29%\"><p>35 mg BSA\/mL<\/p><\/td><\/tr><tr><td width=\"38%\"><p><a href=\"https:\/\/biovanix.com\/fr\/product\/prosep-mabpure-a-lx-protein-a-agarose-chromatography-media\/\">Prosep MabPure A LX<\/a><\/p><\/td><td width=\"29%\"><p>60 mg lgG\/mL<\/p><\/td><\/tr><tr><td width=\"38%\"><p><a title=\"MMC Prosep Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/mmc-prosep-agarose-chromatography-media\/\">Prosep MMC<\/a><\/p><\/td><td width=\"29%\"><p>45 mg BSA\/mL<\/p><\/td><\/tr><tr><td width=\"38%\"><p><a title=\"MMC HPR(LS) Prosep Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/mmc-hpr-ls-prosep-agarose-chromatography-media\/\">Prosep MMC HPR (LS)<\/a><\/p><\/td><td width=\"29%\">35 mg BSA\/mL<\/td><\/tr><tr><td width=\"38%\"><p><a title=\"MMC HPR(HS) Prosep Agarose Chromatography Media\" href=\"https:\/\/biovanix.com\/fr\/product\/mmc-hprhs-prosep-agarose-chromatography-media\/\">Prosep MMC HPR (HS)<\/a><\/p><\/td><td width=\"29%\">50 mg BSA\/mL<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7b35a8d e-flex e-con-boxed e-con e-parent\" data-id=\"7b35a8d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3f6a557 elementor-widget elementor-widget-text-editor\" data-id=\"3f6a557\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><span style=\"color: #33615a;\"><strong>Milieux de chromatographie par \u00e9change d'ions<\/strong><\/span><\/h2><table width=\"576\"><tbody><tr><td width=\"113\"><strong>Produit<\/strong><\/td><td width=\"189\"><strong>Capacit\u00e9 de liaison dynamique<\/strong><\/td><td width=\"274\"><strong>Application<\/strong><\/td><\/tr><tr><td width=\"113\"><a title=\"DEAE 6FF milieu de chromatographie en agarose IEX \u00e0 anions faibles\" href=\"https:\/\/biovanix.com\/fr\/product\/deae-6ff-agarose-chromatography-media\/\">DEAE 6FF<\/a><\/td><td width=\"189\">60 mg BSA\/mL<\/td><td rowspan=\"3\" width=\"274\">Milieu d'\u00e9change d'anions faible :<br \/>Haute applicabilit\u00e9 (FF)<br \/>Haute r\u00e9solution (HP)<br \/>Grande capacit\u00e9 (XL)<\/td><\/tr><tr><td width=\"113\"><a title=\"DEAE 6HP Weak Anion IEX Agarose Chromatography Media (milieu de chromatographie en agarose DEAE 6HP \u00e0 anions faibles)\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-deae-6hp\/\">DEAE 6HP<\/a><\/td><td width=\"189\">60 mg BSA\/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"DEAE 6XL milieu de chromatographie en agarose IEX \u00e0 anions faibles\" href=\"https:\/\/biovanix.com\/fr\/product\/deae-6xl-agarose-chromatography-media\/\">DEAE 6XL<\/a><\/td><td width=\"189\">120 mg BSA\/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"Q 6FF Agarose Strong Anion IEX Support de chromatographie\" href=\"https:\/\/biovanix.com\/fr\/product\/q-6ff-agarose-chromatography-media\/\">Q 6FF<\/a><\/td><td width=\"189\">50 mg BSA\/mL<\/td><td rowspan=\"3\" width=\"274\">Milieux d'\u00e9change d'anions forts :<br \/>Haute applicabilit\u00e9 (FF)<br \/>Haute r\u00e9solution (HP)<br \/>Grande capacit\u00e9 (XL)<\/td><\/tr><tr><td width=\"113\"><a title=\"Q 6HP Strong Anion IEX Agarose Chromatography Media\" href=\"https:\/\/biovanix.com\/fr\/product\/q-6hp-agarose-chromatography-media\/\">Q 6HP<\/a><\/td><td width=\"189\">70 mg BSA\/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"Q 6XL Strong Anion IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/q-6xl-milieu-de-chromatographie-en-agarose\/\">Q 6XL<\/a><\/td><td width=\"189\">140 mg BSA\/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"CM 6FF Milieu de chromatographie en agarose IEX \u00e0 cations faibles\" href=\"https:\/\/biovanix.com\/fr\/product\/cm-6ff-milieu-de-chromatographie-en-agarose\/\">CM 6FF<\/a><\/td><td width=\"189\">100 mg LZM \/mL<\/td><td rowspan=\"3\" width=\"274\">Milieu d'\u00e9change cationique faible :<br \/>Haute applicabilit\u00e9 (FF)<br \/>Haute r\u00e9solution (HP)<br \/>Grande capacit\u00e9 (XL)<\/td><\/tr><tr><td width=\"113\"><a title=\"CM 6HP Weak Cation IEX Agarose Chromatography Media (milieu de chromatographie sur agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/cm-6hp-agarose-chromatography-media\/\">CM 6HP<\/a><\/td><td width=\"189\">120 mg LZM \/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"CM 6XL milieu de chromatographie en agarose IEX \u00e0 cations faibles\" href=\"https:\/\/biovanix.com\/fr\/product\/cm-6xl-milieu-de-chromatographie-en-agarose\/\">CM 6XL<\/a><\/td><td width=\"189\">120 mg LZM \/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"SP 6FF Milieu de chromatographie en agarose IEX \u00e0 cations fortes\" href=\"https:\/\/biovanix.com\/fr\/product\/sp-6ff-agarose-chromatography-media\/\">SP 6FF<\/a><\/td><td width=\"189\">130 mg LZM \/mL<\/td><td rowspan=\"3\" width=\"274\">Milieu d'\u00e9change cationique fort :<br \/>Haute applicabilit\u00e9 (FF)<br \/>Haute r\u00e9solution (HP)<br \/>Grande capacit\u00e9 (XL)<\/td><\/tr><tr><td width=\"113\"><a title=\"SP 6HP Strong Cation IEX Agarose Chromatography Media\" href=\"https:\/\/biovanix.com\/fr\/product\/sp-6hp-milieu-de-chromatographie-en-agarose\/\">SP 6HP<\/a><\/td><td width=\"189\">160 mg LZM \/mL<\/td><\/tr><tr><td width=\"113\"><a title=\"SP 6XL Strong Cation IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/sp-6xl-agarose-chromatography-media\/\">SP 6XL<\/a><\/td><td width=\"189\">160 mg LZM \/mL<\/td><\/tr><\/tbody><\/table><table style=\"font-size: 16px; font-style: normal;\" width=\"643\"><tbody><tr><td width=\"251\"><p><strong>Produit<\/strong><\/p><\/td><td width=\"196\"><p><strong>Capacit\u00e9 de liaison dynamique<\/strong><\/p><\/td><td width=\"196\"><p><strong>Application<\/strong><\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Prosep DEAE Weak Anion IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-deae-agarose-chromatography-media\/\">Prosep DEAE<\/a><\/p><\/td><td width=\"196\"><p>90 mg BSA\/mL<\/p><\/td><td rowspan=\"7\" width=\"196\"><p>Grande rigidit\u00e9<\/p><p>D\u00e9bit \u00e9lev\u00e9<\/p><p>Haute r\u00e9solution<\/p><p>Chargement rapide<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Milieu de chromatographie Prosep Q Agarose Strong Anion IEX\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-q-agarose-strong-anion-iex-chromatography-media\/\">Prosep Q<\/a><\/p><\/td><td width=\"196\"><p>120 mg BSA\/mL<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Prosep SP Strong Cation IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-sp-agarose-chromatography-media\/\">Prosep SP<\/a><\/p><\/td><td width=\"196\"><p>120 mg de lysozyme\/mL<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Prosep DEAE HPR Weak Anion IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-deae-hpr-agarose-chromatography-media\/\">Prosep DEAE HPR<\/a><\/p><\/td><td width=\"196\"><p>35 mg BSA\/mL<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Milieu de chromatographie Prosep Q HPR Agarose Strong Anion IEX\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-q-hpr-agarose-strong-anion-iex-chromatography-media\/\">Prosep Q HPR<\/a><\/p><\/td><td width=\"196\"><p>45 mg BSA\/mL<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Milieu de chromatographie en agarose Prosep CM HPR Weak Cation IEX\" href=\"https:\/\/biovanix.com\/fr\/product\/medias-de-chromatographie-prosep-cm-hpr-agarose\/\">Prosep CM HPR<\/a><\/p><\/td><td width=\"196\"><p>75 mg de lysozyme\/mL<\/p><\/td><\/tr><tr><td width=\"251\"><p><a title=\"Prosep SP HPR Strong Cation IEX Agarose Chromatography Media (milieu de chromatographie en agarose)\" href=\"https:\/\/biovanix.com\/fr\/product\/prosep-sp-hpr-agarose-chromatography-media\/\">Prosep SP HPR<\/a><\/p><\/td><td width=\"196\"><p>70 mg de lysozyme\/mL<\/p><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-9117743 e-flex e-con-boxed e-con e-parent\" data-id=\"9117743\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-40aad24 elementor-widget elementor-widget-text-editor\" data-id=\"40aad24\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><span style=\"color: #33615a;\">Milieux de chromatographie hydrophobe<\/span><\/h2><table width=\"576\"><tbody><tr><td width=\"122\"><strong>Produit<\/strong><\/td><td width=\"189\"><strong>Capacit\u00e9 de liaison dynamique<\/strong><\/td><td width=\"265\"><strong>Application<\/strong><\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Butyl 4FF\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-hydrophobe-butyl-4-ff\/\">Butyl 4FF<\/a><\/td><td width=\"189\">20 mg BSA\/mL<\/td><td rowspan=\"2\" width=\"265\">Faiblement hydrophobe<br \/>Convient pour la s\u00e9paration et la purification de prot\u00e9ines aliphatiques<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Butyl 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-hydrophobe-butyl-6hp\/\">Butyl 6HP<\/a><\/td><td width=\"189\">25 mg BSA\/mL<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Phenyl 6FF HS\" href=\"https:\/\/biovanix.com\/fr\/product\/phenyl-6ff-hs-hydrophobic-agarose-chromatography-media\/\">Ph\u00e9nyl 6FF(HS)<\/a><\/td><td width=\"189\">30 mg BSA\/mL<\/td><td rowspan=\"3\" width=\"265\">Hydrophobicit\u00e9 \u00e9lev\u00e9e  Convient \u00e0 la s\u00e9paration et \u00e0 la purification de prot\u00e9ines aromatiques (telles que les anticorps monoclonaux)<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Phenyl 6FF LS\" href=\"https:\/\/biovanix.com\/fr\/product\/phenyl-6ff-ls-hydrophobic-agarose-chromatography-media\/\">Ph\u00e9nyl 6FF (LS)<\/a><\/td><td width=\"189\">15 mg BSA\/mL<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Phenyl 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-hydrophobe-phenyl-6hp\/\">Ph\u00e9nyle 6HP<\/a><\/td><td width=\"189\">20 mg BSA\/mL<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Octyl 4FF\" href=\"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-hydrophobe-octyl-4ff\/\">Octyl 4FF<\/a><\/td><td width=\"189\">8 mg BSA\/mL<\/td><td rowspan=\"2\" width=\"265\">Hydrophobie moyenne<br \/>Convient pour la s\u00e9paration et la purification de prot\u00e9ines fortement lipophiles<\/td><\/tr><tr><td width=\"122\"><a title=\"Milieu de chromatographie en agarose hydrophobe Octyl 6HP\" href=\"https:\/\/biovanix.com\/fr\/product\/octyl-6hp-hydrophobic-agarose-chromatography-media\/\">Octyl 6HP<\/a><\/td><td width=\"189\">8 mg BSA\/mL<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-98d0ccd e-flex e-con-boxed e-con e-parent\" data-id=\"98d0ccd\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-9f95ca4 elementor-widget elementor-widget-text-editor\" data-id=\"9f95ca4\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h1>Foire Aux Questions (FAQ) : Milieux de Chromatographie sur Gel d'Agarose<\/h1><h2>1. Caract\u00e9ristiques techniques et s\u00e9lection des m\u00e9dias<\/h2><p data-path-to-node=\"4\"><b>Qu'est-ce qui distingue les milieux d'agarose des matrices \u00e0 base de polym\u00e8res ou de silice ?<\/b><\/p><p data-path-to-node=\"4\"><b>A\u00a0:<\/b> L'agarose est une matrice polysaccharide hydrophile d'origine naturelle. Son principal avantage par rapport aux options synth\u00e9tiques est sa <b>hydrophilie intrins\u00e8que et faible liaison non sp\u00e9cifique (NSB)<\/b>. Cette caract\u00e9ristique minimise l'adsorption des biomol\u00e9cules non cibl\u00e9es, garantissant ainsi une r\u00e9cup\u00e9ration \u00e9lev\u00e9e des prot\u00e9ines et pr\u00e9servant l'activit\u00e9 biologique des cibles sensibles.<\/p><ul data-path-to-node=\"5\"><li><p data-path-to-node=\"5,0,0\"><b>Contre Silica:<\/b> L'agarose offre une stabilit\u00e9 chimique sup\u00e9rieure dans des conditions alcalines, permettant l'utilisation d'hydroxyde de sodium (NaOH) pour le nettoyage en place (NEP), qui peut d\u00e9grader la silice.<\/p><\/li><li><p data-path-to-node=\"5,1,0\"><b>Contre. Polym\u00e8res synth\u00e9tiques :<\/b> Alors que les polym\u00e8res rigides (par exemple, polystyr\u00e8ne-divinylbenz\u00e8ne) offrent une tol\u00e9rance \u00e0 la pression plus \u00e9lev\u00e9e, ils n\u00e9cessitent souvent une hydrophilisation de surface. L'agarose est naturellement hydrophile, ce qui r\u00e9duit le risque de d\u00e9naturation des prot\u00e9ines, bien qu'il fonctionne g\u00e9n\u00e9ralement \u00e0 des limites de pression inf\u00e9rieures \u00e0 celles des billes de polym\u00e8res rigides.<\/p><\/li><\/ul><p data-path-to-node=\"6\"><b>Q : Quelle est la diff\u00e9rence entre l'agarose \u201c native \u201d et l'agarose \u201c r\u00e9ticul\u00e9e \u201d ?<\/b><\/p><p data-path-to-node=\"6\"><b>A\u00a0:<\/b> \u201d Natif \u201c vs \u00ab R\u00e9ticul\u00e9 \u00bb<\/p><ul data-path-to-node=\"7\"><li><p data-path-to-node=\"7,0,0\"><b>Agarose Native :<\/b> La structure du gel est stabilis\u00e9e uniquement par des liaisons hydrog\u00e8ne. Il manque de rigidit\u00e9 m\u00e9canique et se comprime facilement sous pression, ce qui le rend inadapt\u00e9 aux d\u00e9bits industriels. Son application principale est l'\u00e9lectrophor\u00e8se analytique sur gel.<\/p><\/li><li><p data-path-to-node=\"7,1,0\"><b>Agarose r\u00e9ticul\u00e9 :<\/b> Cette variante subit une modification chimique (g\u00e9n\u00e9ralement \u00e0 l'aide de l'\u00e9pichlorhydrine) pour former des liaisons covalentes entre les cha\u00eenes de polysaccharides. Ceci <b>r\u00e9ticulation<\/b> am\u00e9liore consid\u00e9rablement la rigidit\u00e9 m\u00e9canique du talon, ce qui permet d'atteindre les d\u00e9bits \u00e9lev\u00e9s et la tol\u00e9rance \u00e0 la pression requis pour la fabrication \u00e0 l'\u00e9chelle industrielle (comparable aux normes \u201c Fast Flow \u201d ou \u201c Capto \u201d).<\/p><\/li><\/ul><p data-path-to-node=\"8\"><b>Q : Comment choisir entre les concentrations d&#x27;agarose 4% et 6% ?<\/b><\/p><p data-path-to-node=\"8\"><b>A\u00a0:<\/b> La concentration d'agarose est inversement proportionnelle \u00e0 la taille des pores de la r\u00e9sine :<\/p><ul data-path-to-node=\"9\"><li><p data-path-to-node=\"9,0,0\"><b>Agarose 4% :<\/b> Il pr\u00e9sente une structure de pores plus large, ce qui le rend id\u00e9al pour la purification de biomol\u00e9cules tr\u00e8s volumineuses telles que les virus, les plasmides et les complexes prot\u00e9iques de grande taille. Cependant, il pr\u00e9sente une r\u00e9sistance m\u00e9canique plus faible par rapport \u00e0 des concentrations plus \u00e9lev\u00e9es.<\/p><\/li><li><p data-path-to-node=\"9,1,0\"><b>Agarose 6% :<\/b> Les pores sont l\u00e9g\u00e8rement plus petits mais offrent une rigidit\u00e9 m\u00e9canique am\u00e9lior\u00e9e. Il \u00e9quilibre la capacit\u00e9 et les performances de d\u00e9bit, servant de r\u00e9f\u00e9rence dans l'industrie pour la purification g\u00e9n\u00e9rale des prot\u00e9ines, y compris les anticorps monoclonaux (AcM) et les prot\u00e9ines recombinantes.<\/p><\/li><\/ul><h2>2. Performances comparatives et validation<\/h2><p data-path-to-node=\"11\"><b>Q: Comment vos m\u00e9dias se comparent-ils aux r\u00e9f\u00e9rences de l'industrie telles que Cytiva (GE) ?<\/b><\/p><p data-path-to-node=\"11\"><b>A\u00a0:<\/b> Gr\u00e2ce aux progr\u00e8s significatifs de la technologie de fabrication au cours de la derni\u00e8re d\u00e9cennie, nos m\u00e9dias ont atteint une parit\u00e9 \u00e9lev\u00e9e avec les r\u00e9f\u00e9rences \u00e9tablies.<\/p><ul data-path-to-node=\"12\"><li><p data-path-to-node=\"12,0,0\"><b>Performance :<\/b> Nos r\u00e9sines \u00e9quivalentes \u201c Fast Flow \u201d d\u00e9montrent des performances comparables <b>Capacit\u00e9 de liaison dynamique (CLD)<\/b>, r\u00e9solution et caract\u00e9ristiques de d\u00e9bit-pression \u00e0 Sepharose\u2122 Fast Flow.<\/p><\/li><li><p data-path-to-node=\"12,1,0\"><b>Avantage commercial :<\/b> Les principaux facteurs de diff\u00e9renciation sont <b>rentabilit\u00e9<\/b> et <b>r\u00e9silience de la cha\u00eene d'approvisionnement<\/b>, offrant des d\u00e9lais de livraison consid\u00e9rablement plus courts par rapport aux marques import\u00e9es.<\/p><\/li><\/ul><p data-path-to-node=\"13\"><b>Q: Puis-je utiliser votre m\u00e9dia comme un remplacement direct \u201cplug-and-play\u201d de Sepharose FF ?<\/b><\/p><p data-path-to-node=\"13\"><b>A\u00a0:<\/b> Bien que la matrice de base (agarose r\u00e9ticul\u00e9) soit chimiquement analogue, nous recommandons une formelle <b>\u00c9tude de comparabilit\u00e9<\/b> avant la mise en \u0153uvre \u00e0 grande \u00e9chelle. Les attributs qualit\u00e9 critiques (AQC) cl\u00e9s \u00e0 valider comprennent :<\/p><ol start=\"1\" data-path-to-node=\"14\"><li><p data-path-to-node=\"14,0,0\"><b>Distribution granulom\u00e9trique<\/b> V\u00e9rification que la taille du maillage correspond (par exemple, ~90 \u03bcm) afin d'assurer une contre-pression constante.<\/p><\/li><li><p data-path-to-node=\"14,1,0\"><b>Densit\u00e9 du ligand :<\/b> Confirmation que la densit\u00e9 des groupes fonctionnels soutient la capacit\u00e9 de liaison et le profil d'\u00e9lution requis.<\/p><\/li><li><p data-path-to-node=\"14,2,0\"><b>Liaison non sp\u00e9cifique :<\/b> \u00c9valuation des niveaux de liaison de fond pour garantir que l'\u00e9limination des impuret\u00e9s respecte les sp\u00e9cifications.<\/p><\/li><\/ol><p data-path-to-node=\"15\"><b>R : Quel soutien r\u00e9glementaire est fourni pour la production BPF ?<\/b><\/p><p data-path-to-node=\"15\"><b>A\u00a0:<\/b> Nous fournissons un support complet pour la fabrication conforme aux BPF. Notre syst\u00e8me qualit\u00e9 est <b>Certifi\u00e9 ISO 9001<\/b>.<\/p><h2>3. Lignes directrices op\u00e9rationnelles et maintenance<\/h2><p data-path-to-node=\"17\"><b>Q: Quel est le protocole de Nettoyage en Place (NEP) recommand\u00e9 ?<\/b><\/p><p data-path-to-node=\"17\"><b>A\u00a0:<\/b> L'agarose r\u00e9ticul\u00e9 pr\u00e9sente une excellente stabilit\u00e9 alcaline. Le protocole CIP standard utilise <b>0,1 M \u00e0 1,0 M de NaOH<\/b>.<\/p><ul data-path-to-node=\"18\"><li><p data-path-to-node=\"18,0,0\"><b>Param\u00e8tres :<\/b> Une dur\u00e9e de contact de 30 \u00e0 60 minutes est standard.<\/p><\/li><li><p data-path-to-node=\"18,1,0\"><b>M\u00e9canisme\u00a0:<\/b> Ceci hydrolyse efficacement les prot\u00e9ines pr\u00e9cipit\u00e9es, saponifie les lipides et inactive les endotoxines et les virus sans compromettre l'int\u00e9grit\u00e9 du squelette d'agarose.<\/p><\/li><\/ul><p data-path-to-node=\"19\"><b>Q: Quel est le cycle de vie typique de la r\u00e9sine ?<\/b><\/p><p data-path-to-node=\"19\"><b>A\u00a0:<\/b> Dans un proc\u00e9d\u00e9 BPF bien contr\u00f4l\u00e9, les milieux \u00e0 base d'agarose r\u00e9ticul\u00e9 supportent typiquement <b>100 \u00e0 200 cycles de purification<\/b>. La dur\u00e9e de vie de la r\u00e9sine est g\u00e9n\u00e9ralement limit\u00e9e par l'encrassement irr\u00e9versible (perte progressive de la capacit\u00e9 dynamique) ou par l'augmentation de la contre-pression de la colonne due \u00e0 l'accumulation de particules, plut\u00f4t que par la d\u00e9gradation chimique de la bille elle-m\u00eame.<\/p><p data-path-to-node=\"20\"><b>Q : Quelles sont les exigences de stockage pour les m\u00e9dias ?<\/b><\/p><p data-path-to-node=\"20\"><b>A\u00a0:<\/b> Les milieux \u00e0 base d'agarose doivent \u00eatre conserv\u00e9s dans une solution bact\u00e9riostatique, g\u00e9n\u00e9ralement <b>20% \u00c9thanol<\/b>, \u00e0 des temp\u00e9ratures comprises entre <b>4\u00b0C et 30\u00b0C<\/b>.<\/p><ul data-path-to-node=\"21\"><li><p data-path-to-node=\"21,0,0\"><b>Avertissement critique :<\/b> <b>Ne congelez pas les billes d'agarose.<\/b> Le gel congel\u00e9 provoque la formation de cristaux de glace \u00e0 l'int\u00e9rieur du r\u00e9seau de pores, ce qui fragilise la structure du gel et d\u00e9truit irr\u00e9versiblement les performances chromatographiques du milieu.<\/p><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-ad99f48 e-flex e-con-boxed e-con e-parent\" data-id=\"ad99f48\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-198b94b e-con-full e-flex e-con e-child\" data-id=\"198b94b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-118cad91 elementor-widget elementor-widget-text-editor\" data-id=\"118cad91\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">Vous ne trouvez pas le produit dont vous avez besoin ? Contactez nous directement.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c389c e-con-full e-flex e-con e-child\" data-id=\"c389c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-6d375b98 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"6d375b98\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Nous contacter<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Agarose Chromatography Media Biovanix Agarose Media is a conventional biological separation medium crafted from highly crosslinked agarose microspheres. It utilizes high-performance agarose raw materials sourced from abroad to maintain a consistent level of product quality. 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