{"id":4878,"date":"2025-02-08T13:13:16","date_gmt":"2025-02-08T13:13:16","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4878"},"modified":"2025-02-08T15:04:24","modified_gmt":"2025-02-08T15:04:24","slug":"resine-de-chromatographie-daffinite-oligo-dt25","status":"publish","type":"page","link":"https:\/\/biovanix.com\/fr\/oligo-dt25-affinity-chromatography-resin\/","title":{"rendered":"R\u00e9sine de chromatographie d'affinit\u00e9 Oligo dT(25)"},"content":{"rendered":"
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R\u00e9sine de chromatographie d'affinit\u00e9 Oligo dT(25)<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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R\u00e9sine d'affinit\u00e9 Biovanix Oligo dT(25)<\/strong>\u00a0est une solution de pointe con\u00e7ue pour la purification et l'isolement de l'ARNm \u00e0 partir de processus de fabrication de transcription in vitro (IVT). Cette r\u00e9sine avanc\u00e9e capture s\u00e9lectivement l'ARNm via sa queue polyad\u00e9nyl\u00e9e (polyA), le s\u00e9parant ainsi efficacement des autres composants de la r\u00e9action de transcription, tels que les enzymes et l'ADN plasmidique.<\/p>

Parfaitement adapt\u00e9 \u00e0 la purification \u00e0 grande \u00e9chelle en aval,\u00a0R\u00e9sine d'affinit\u00e9 Biovanix Oligo dT(25)<\/strong>\u00a0est un facilitateur cl\u00e9 pour les applications de vaccins et de th\u00e9rapies g\u00e9niques, offrant une s\u00e9lectivit\u00e9 et une capacit\u00e9 in\u00e9gal\u00e9es pour r\u00e9pondre aux exigences de la fabrication biopharmaceutique moderne.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Caract\u00e9ristiques<\/h2>

1. Capture s\u00e9lective d'ARNm<\/strong><\/h4>
  • Sp\u00e9cificit\u00e9 de la Queue PolyA :<\/strong>\u00a0Cible l'ARNm avec une grande s\u00e9lectivit\u00e9, assurant l'\u00e9limination efficace de l'ADN plasmidique et d'autres composants IVT.<\/li>
  • \u00c9lution simple par sel et eau :<\/strong>\u00a0Proc\u00e9d\u00e9 de purification simplifi\u00e9 avec une complexit\u00e9 minimale.<\/li><\/ul>

    2. Solution \u00e9volutive<\/strong><\/h4>
    • Excellente scalabilit\u00e9 :<\/strong>\u00a0Con\u00e7u pour une transition transparente de l'\u00e9chelle du laboratoire \u00e0 la production \u00e0 grande \u00e9chelle.<\/li>
    • Haute Capacit\u00e9 :<\/strong>\u00a0R\u00e9pond aux exigences de la fabrication d'ARNm \u00e0 haut rendement.<\/li><\/ul>

      3. Non d\u00e9riv\u00e9 d'animaux<\/strong><\/h4>
      • Proc\u00e9d\u00e9 de Fabrication Synth\u00e9tique :<\/strong>\u00a0Les ligands oligo Biovanix sont exempts de composants d'origine animale, garantissant ainsi la conformit\u00e9 aux exigences r\u00e9glementaires et aux normes \u00e9thiques.<\/li><\/ul>

        4. Workflow simplifi\u00e9<\/strong><\/h4>
        • Complexit\u00e9 r\u00e9duite :<\/strong>\u00a0\u00c9limine le besoin de plusieurs \u00e9tapes de purification, maximisant ainsi l'efficacit\u00e9 et r\u00e9duisant la complexit\u00e9 des processus en aval.<\/li>
        • Performances constantes<\/strong>\u00a0Des r\u00e9sultats fiables sur diff\u00e9rentes \u00e9chelles et lots de production.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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          Sp\u00e9cification<\/strong><\/h2>
          Caract\u00e9ristique<\/strong><\/td>Description<\/strong><\/td><\/tr>
          Matrice de soutien<\/strong><\/td>Poly(styr\u00e8ne-divinylbenz\u00e8ne) r\u00e9ticul\u00e9<\/td><\/tr>
          Taille moyenne des particules<\/strong><\/td>50 \u00b5m<\/td><\/tr>
          Taille moyenne des pores<\/strong><\/td>200 nm<\/td><\/tr>
          Fonctionnalit\u00e9 de surface<\/strong><\/td>poly(dT) 25-m\u00e8re avec lieur propri\u00e9taire<\/td><\/tr>
          Densit\u00e9 du ligand<\/strong><\/td>0,3 \u00b5mol\/ml<\/td><\/tr>
          R\u00e9sistance m\u00e9canique<\/strong><\/td>70 bars (1 000 psi ; 7 MPa)<\/td><\/tr>
          Stabilit\u00e9 thermique<\/strong><\/td>permet la d\u00e9naturation d'\u00e9chantillons \u00e0 65\u00b0C si n\u00e9cessaire<\/td><\/tr>
          Gamme de pH<\/strong><\/td>2-13<\/td><\/tr>
          Gamme de force ionique<\/strong><\/td>0 \u00e0 5 M, tous les sels courants<\/td><\/tr>
          R\u00e9sistance chimique<\/strong><\/td>Les agents couramment utilis\u00e9s pour la purification de l'ARNm comprennent le NaOH 0,5 M, le MgCl\u2082 2 M et l'EDTA 20 mM. L'eau, l'alcool 0 \u00e0 100%, l'ac\u00e9tonitrile, l'acide ac\u00e9tique 2 M, l'HCl 1 M et d'autres solvants organiques courants<\/td><\/tr>
          Stockage<\/strong><\/td>18-20% \u00e9thanol<\/td><\/tr><\/tbody><\/table>

          \u00a0<\/h3>

          R\u00e9sistance chimique et thermique<\/strong><\/h3>
          Gamme de pH<\/strong><\/td>2\u201313<\/td><\/tr>
          Gamme de force ionique<\/strong><\/td>0 \u00e0 5 M, tous les sels courants<\/td><\/tr>
          Additifs de tampon<\/strong><\/td>Les agents courants pour la purification de l'ARNm comprennent 0,5 M de NaOH, 2 mM de MgCl2, 20 mM d'EDTA.

          \u00a0<\/p>

          Ne pas exposer \u00e0 des oxydants forts (tels que l'hypochlorite), des acides oxydants (tels que l'acide nitrique), des agents r\u00e9ducteurs forts (tels que le sulfite), l'ac\u00e9tone, le THF ou l'alcool benzylique.<\/p><\/td><\/tr>

          Solvants<\/strong><\/td>Eau, alcool 0 \u00e0 100%, ac\u00e9tonitrile, acide ac\u00e9tique 2 M, HCl 1 M et autres solvants organiques courants.

          \u00a0<\/p>

          Ne pas exposer aux agents d'oxydation forts (tels que l'hypochlorite), aux acides oxydants (tels que l'acide nitrique), aux agents r\u00e9ducteurs forts (tels que le sulfite), \u00e0 l'ac\u00e9tone, \u00e0 l'alcool benzylique ou au THF.<\/p><\/td><\/tr>

          D\u00e9bit<\/strong><\/td>Ajuster le d\u00e9bit en fonction des performances. Ne pas d\u00e9passer les limites de pression sup\u00e9rieures.<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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          Avantages en un coup d'\u0153il<\/strong><\/h2>

          Haute efficacit\u00e9<\/strong><\/h4>

          Purification rapide et efficace de l'ARNm.<\/p>

          Proc\u00e9d\u00e9 \u00e9volutif<\/strong><\/h4>

          Facilement adaptable \u00e0 la fabrication \u00e0 grande \u00e9chelle.<\/p>

          Conformit\u00e9 r\u00e9glementaire<\/strong><\/h4>

          Proc\u00e9d\u00e9 de fabrication non d\u00e9riv\u00e9 d'animaux et synth\u00e9tique.<\/p>

          Rentable<\/strong><\/h4>

          Un flux de travail simplifi\u00e9 r\u00e9duit la complexit\u00e9 op\u00e9rationnelle et les co\u00fbts.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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          Applications<\/strong><\/h2>

          Production de vaccins<\/strong><\/h4>

          Purification efficace des vaccins \u00e0 ARNm.<\/p>

          Th\u00e9rapie g\u00e9nique<\/strong><\/h4>

          Production \u00e9volutive d'ARN messager pour des applications th\u00e9rapeutiques.<\/p>

          Fabrication IVT<\/strong><\/h4>

          Purification simplifi\u00e9e de l'ARN messager \u00e0 partir de milieux de r\u00e9actions de transcription.<\/p>

          Suppression de l'ADN plasmidique<\/strong><\/h4>

          \u00c9limination efficace de l'ADN plasmidique et des autres composants de la r\u00e9action de transcription in vitro.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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          Pourquoi choisir la r\u00e9sine d'affinit\u00e9 Biovanix Oligo dT(25) ?<\/strong><\/h2>

          Purification efficace d'ARNm<\/strong><\/h4>

          Obtenez de l'ARNm de haute puret\u00e9 avec un minimum d'impuret\u00e9s.<\/p>

          Rentable<\/strong><\/h4>

          La r\u00e9duction de la complexit\u00e9 op\u00e9rationnelle et l'augmentation de la productivit\u00e9 diminuent les co\u00fbts de production globaux.<\/p>

          Conformit\u00e9 r\u00e9glementaire<\/strong><\/h4>

          La fabrication non d\u00e9riv\u00e9e d'animaux et synth\u00e9tique garantit la conformit\u00e9 aux normes mondiales.<\/p>

          Application polyvalente<\/strong><\/h4>

          Id\u00e9al pour un large \u00e9ventail de constructions ARNm utilis\u00e9es dans les vaccins, les th\u00e9rapies g\u00e9niques et autres produits biopharmaceutiques.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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          T\u00e9moignage client<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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          \u201cLa r\u00e9sine chromatographique Biovanix InertShell a transform\u00e9 notre processus de purification des virus. Sa double fonctionnalit\u00e9 et sa haute s\u00e9lectivit\u00e9 ont consid\u00e9rablement am\u00e9lior\u00e9 nos rendements et nos niveaux de puret\u00e9, en faisant un outil essentiel dans nos tests.\u201d<\/em>
          \u2013 Alex, D\u00e9veloppeur, AA Biopharma<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

          Oligo dT(25) Affinity Chromatography Resin Biovanix Oligo dT(25) Affinity Resin\u00a0is a cutting-edge solution designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This advanced resin selectively captures mRNA via its polyadenylated (polyA) tail, effectively separating it from other components of the transcription reaction, such as enzymes and plasmid DNA. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4878","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"Biovanix","author_link":"https:\/\/biovanix.com\/fr\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"Oligo dT(25) Affinity Chromatography Resin Biovanix Oligo dT(25) Affinity Resin\u00a0is a cutting-edge solution designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This advanced resin selectively captures mRNA via its polyadenylated (polyA) tail, effectively separating it from other components of the transcription reaction, such as enzymes and plasmid DNA.\u2026","aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t