{"id":4891,"date":"2025-02-08T14:35:45","date_gmt":"2025-02-08T14:35:45","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4891"},"modified":"2025-02-08T15:05:47","modified_gmt":"2025-02-08T15:05:47","slug":"resine-de-chromatographie-a-coeur-plein-inerte","status":"publish","type":"page","link":"https:\/\/biovanix.com\/fr\/inertshell-core-shell-chromatography-resin\/","title":{"rendered":"R\u00e9sine de chromatographie InertShell Core-Shell"},"content":{"rendered":"
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R\u00e9sine de chromatographie InertShell Core-Shell<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Pour la purification d'Ad\u00e9noviridae ADV<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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R\u00e9sine de chromatographie Biovanix InertShell<\/strong>\u00a0est une r\u00e9sine bas\u00e9e sur une technologie r\u00e9volutionnaire de c\u0153ur-\u00e9corce, con\u00e7ue pour la purification des virus et des grandes biomol\u00e9cules. Combinant la s\u00e9paration par exclusion de taille avec la chromatographie d'affinit\u00e9, cette r\u00e9sine avanc\u00e9e capture et isole efficacement les grandes biomol\u00e9cules tout en permettant aux contaminants plus petits de passer et de se lier au c\u0153ur. Cette double fonctionnalit\u00e9 garantit des r\u00e9sultats de haute puret\u00e9 dans le traitement en aval.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Caract\u00e9ristiques<\/h2>

1. Technologie C\u0153ur-\u00c9corce<\/strong><\/h4>
  • Double fonctionnalit\u00e9 :<\/strong>\u00a0Effectue simultan\u00e9ment une s\u00e9paration par exclusion de taille et une chromatographie d'affinit\u00e9.<\/li>
  • S\u00e9paration efficace :<\/strong>\u00a0Les biomol\u00e9cules de grande taille (par exemple, des virus inactiv\u00e9s) sont collect\u00e9es dans la fraction de percolation (mode FT), tandis que les contaminants (<700 kDa) se lient aux ligands internes.<\/li>
  • Conception optimis\u00e9e :<\/strong>\u00a0La coque ext\u00e9rieure est hydrophile et neutre, emp\u00eachant les interactions ind\u00e9sirables et garantissant des d\u00e9bits \u00e9lev\u00e9s.<\/li><\/ul>

    2. Composition avanc\u00e9e des mat\u00e9riaux<\/strong><\/h4>
    • Base de polym\u00e8re :<\/strong>\u00a0Fabriqu\u00e9 \u00e0 partir de microsph\u00e8res de m\u00e9thacrylate polym\u00e8re pour une durabilit\u00e9 robuste et une stabilit\u00e9 chimique.<\/li>
    • Ligand actif :<\/strong>\u00a0Le ligand octylamine dans le c\u0153ur offre \u00e0 la fois des capacit\u00e9s d'\u00e9change anionique et d'interaction hydrophobe.<\/li>
    • Structure poreuse :<\/strong>\u00a0La coquille a une taille de pores de 50 \u00e0 100 nm, tandis que le c\u0153ur varie de 200 \u00e0 500 nm, assurant une s\u00e9paration efficace bas\u00e9e sur la taille mol\u00e9culaire.<\/li><\/ul>

      3. Haute puret\u00e9 et efficacit\u00e9<\/strong><\/h4>
      • Capture s\u00e9lective :<\/strong>\u00a0\u00c9limine efficacement les impuret\u00e9s telles que les prot\u00e9ines de cellules h\u00f4tes, les fragments d'ADN, les endotoxines et l'albumine.<\/li>
      • Haute Capacit\u00e9 :<\/strong>\u00a0Con\u00e7u pour g\u00e9rer des proc\u00e9d\u00e9s de purification \u00e0 grande \u00e9chelle avec une grande efficacit\u00e9 et \u00e9volutivit\u00e9.<\/li><\/ul>

        4. Conformit\u00e9 et s\u00e9curit\u00e9<\/strong><\/h4>
        • D\u00e9riv\u00e9 non animal<\/strong>\u00a0fabriqu\u00e9 selon des proc\u00e9d\u00e9s synth\u00e9tiques, garantissant la conformit\u00e9 avec les normes r\u00e9glementaires et les exigences \u00e9thiques.<\/li>
        • Performances stables<\/strong>\u00a0Fiable sur diff\u00e9rentes \u00e9chelles de production, garantissant des r\u00e9sultats constants.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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          Sp\u00e9cification<\/strong><\/h2>
          \u00a0<\/td>Coque inerte Biovanix<\/strong><\/td>Concurrent 700<\/strong><\/td><\/tr>
          Matrice<\/td>Polyacrylate<\/td>Agarose hautement r\u00e9ticul\u00e9<\/td><\/tr>
          Ligand<\/td>Octylamine<\/td>Octylamine<\/td><\/tr>
          Taille moyenne des particules<\/td>50-150 \u03bcm<\/td>50-150 \u03bcm<\/td><\/tr>
          Densit\u00e9 du ligand<\/td>0,10-0,20 mmol\/mL<\/td>0,04-0,085 mmol\/mL<\/td><\/tr>
          Capacit\u00e9 de liaison\u00b9<\/td>20 mg d'albumine s\u00e9rique bovine\/mL de r\u00e9sine<\/td>12 mg BSa\/mL r\u00e9sine<\/td><\/tr>
          Pression de fonctionnement<\/td>\u22641,0 MPa<\/td>\u22640,3 MPa<\/td><\/tr>
          D\u00e9bit op\u00e9rationnel<\/td>100-600 cm\/h<\/td>100-600 cm\/h<\/td><\/tr>
          Stabilit\u00e9 du pH<\/td>3-13<\/td>3-13<\/td><\/tr>
          Temp\u00e9rature<\/td>4-30\u2103<\/td>4-30\u2103<\/td><\/tr>
          Stabilit\u00e9 chimique<\/td>Tous les tampons aqueux couramment utilis\u00e9s : hydroxyde de sodium (NaOH) \u00e0 1 M\u00b2, chlorhydrate de guanidine \u00e0 6 M, isopropanol 30% et \u00e9thanol 70%.<\/td><\/tr>
          Stockage<\/td>\u00c9thanol 20% entre 4 \u00b0C et 25 \u00b0C<\/td><\/tr><\/tbody><\/table>
          1. Capacit\u00e9 de liaison dynamique mesur\u00e9e au moment de la perc\u00e9e \u00e0 51 TP3T, \u00e0 un d\u00e9bit de 76 cm\/h, sur des colonnes de \u03c6 10 \u00d7 13 mm et d'une capacit\u00e9 de 1 ml. Le tampon \u00e9tait compos\u00e9 de 1,0 mg\/ml de BSA dans une solution de NaCl \u00e0 50 mM, pH 0.<\/em><\/li>
          2. Aucun changement significatif de la capacit\u00e9 ionique et de la teneur en carbone apr\u00e8s un stockage d'une semaine dans du NaOH 1 M \u00e0 25\u00b0C.<\/em><\/li><\/ol>

            Comparativement au concurrent 700, l'\u00e9paisseur du c\u0153ur dans Biovanix Inert Shell (0,5-1,0 \u03bcm) est plus faible que celle du concurrent 700 (5 \u03bcm). Biovanix Inert Shell favorise le transfert de masse rapide des prot\u00e9ines impures vers le c\u0153ur du milieu pour la capture, comme les prot\u00e9ines cellulaires de l'h\u00f4te, les fragments d'ADN, les endotoxines, le s\u00e9rum. Biovanix Inert Shell pr\u00e9sente un rendement plus \u00e9lev\u00e9 en prot\u00e9ines diverses. Gr\u00e2ce \u00e0 la structure macroporeuse (200-500 nm) du c\u0153ur de Biovanix Inert Shell, on peut rapidement \u00e9liminer les prot\u00e9ines diverses lors du nettoyage en place (NEP). Biovanix Inert Shell a \u00e9galement une dur\u00e9e de vie plus longue dans le processus de purification. Dans les \u00e9tudes de vaccins animaux, les performances de Biovanix Inert Shell peuvent \u00eatre r\u00e9p\u00e9t\u00e9es plus de 30 fois avec peu de changement dans leurs propri\u00e9t\u00e9s.<\/p><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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            Applications<\/strong><\/h2>

            Purification de virus<\/strong><\/h4>

            Isolement efficace des virus inactiv\u00e9s pour les vaccins et les th\u00e9rapies g\u00e9niques.<\/p>

            S\u00e9paration de Grandes Biomol\u00e9cules<\/strong><\/h4>

            Convient \u00e0 la purification de grandes prot\u00e9ines et de complexes.<\/p>

            \u00c9limination des prot\u00e9ines de la cellule h\u00f4te<\/strong><\/h4>

            \u00c9limination efficace des prot\u00e9ines de cellules h\u00f4tes et autres impuret\u00e9s.<\/p>

            Production de vaccins<\/strong><\/h4>

            Optimisation des proc\u00e9d\u00e9s de purification en aval pour les vaccins \u00e0 ARNm et les vaccins \u00e0 base de vecteurs viraux.<\/p>

            Th\u00e9rapie g\u00e9nique<\/strong><\/h4>

            Production \u00e9volutive de vecteurs viraux pour les applications de th\u00e9rapie g\u00e9nique.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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            Pourquoi choisir la r\u00e9sine chromatographique Biovanix InertShell ?<\/strong><\/h2>

            Puret\u00e9 am\u00e9lior\u00e9e<\/strong><\/h4>

            \u00c9limination efficace des impuret\u00e9s pour atteindre une ultra-haute puret\u00e9 dans la purification de virus et de biomol\u00e9cules.<\/p>

            Fonctionnement bi-mode<\/strong><\/h4>

            Combine la chromatographie d'exclusion de taille et la chromatographie d'affinit\u00e9 pour un contr\u00f4le polyvalent et pr\u00e9cis.<\/p>

            Solution \u00e9volutive<\/strong><\/h4>

            Id\u00e9al pour les processus de fabrication \u00e0 l'\u00e9chelle du laboratoire et \u00e0 grande \u00e9chelle.<\/p>

            Rentable<\/strong><\/h4>

            Des processus simplifi\u00e9s et une efficacit\u00e9 \u00e9lev\u00e9e r\u00e9duisent les co\u00fbts op\u00e9rationnels et am\u00e9liorent la productivit\u00e9.<\/p>

            Conformit\u00e9 r\u00e9glementaire<\/strong><\/h4>

            La fabrication \u00e0 partir de composants d'origine non animale et synth\u00e9tiques garantit le respect des normes biopharmaceutiques internationales.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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            T\u00e9moignage client<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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            \u201cLa r\u00e9sine d'affinit\u00e9 Biovanix Oligo dT(25) a r\u00e9volutionn\u00e9 notre processus de purification de l'ARNm. La haute s\u00e9lectivit\u00e9 et la capacit\u00e9 de la r\u00e9sine ont consid\u00e9rablement am\u00e9lior\u00e9 notre rendement et notre puret\u00e9, nous permettant ainsi de r\u00e9pondre \u00e0 la demande croissante de vaccins \u00e0 ARNm.\u201d<\/em>
            \u2013\u00a0John, Ing\u00e9nieur en bioproc\u00e9d\u00e9s, Une biopharmaceutique<\/strong><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

            InertShell Core-Shell Chromatography Resin For Adenoviridae ADV Purification Biovanix InertShell Chromatography Resin\u00a0is a revolutionary core-shell technology-based resin designed for the purification of viruses and large biomolecules. Combining size-exclusion separation with binding chromatography, this advanced resin efficiently captures and isolates large biomolecules while allowing smaller contaminants to pass through and bind within the core. This dual […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4891","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"Biovanix","author_link":"https:\/\/biovanix.com\/fr\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"InertShell Core-Shell Chromatography Resin For Adenoviridae ADV Purification Biovanix InertShell Chromatography Resin\u00a0is a revolutionary core-shell technology-based resin designed for the purification of viruses and large biomolecules. Combining size-exclusion separation with binding chromatography, this advanced resin efficiently captures and isolates large biomolecules while allowing smaller contaminants to pass through and bind within the core. This dual\u2026","aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t