{"id":3806,"date":"2025-01-20T14:31:08","date_gmt":"2025-01-20T14:31:08","guid":{"rendered":"https:\/\/biovanix.com\/?p=3806"},"modified":"2025-01-20T14:47:09","modified_gmt":"2025-01-20T14:47:09","slug":"phases-daffinite-selectivite-en-chromatographie-liquide","status":"publish","type":"post","link":"https:\/\/biovanix.com\/fr\/phases-daffinite-selectivite-en-chromatographie-liquide\/","title":{"rendered":"Phases d'affinit\u00e9 S\u00e9lectivit\u00e9 en chromatographie liquide"},"content":{"rendered":"
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Phases d'affinit\u00e9 S\u00e9lectivit\u00e9<\/span><\/h1>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Les phases d'affinit\u00e9 offrent la sp\u00e9cificit\u00e9 et la s\u00e9lectivit\u00e9 les plus \u00e9lev\u00e9es pour les s\u00e9parations et les purifications biomol\u00e9culaires. Des purifications de plusieurs ordres de grandeur peuvent \u00eatre r\u00e9alis\u00e9es en une seule \u00e9tape. Les s\u00e9parations par affinit\u00e9 permettent souvent d'\u00e9liminer des contaminants difficiles \u00e0 \u00e9liminer \u00e0 l'aide de proc\u00e9dures chromatographiques conventionnelles.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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M\u00e9canisme<\/h3>

La chromatographie d'affinit\u00e9 repose sur un m\u00e9canisme de type \"serrure et cl\u00e9\". Un ligand d'affinit\u00e9, sp\u00e9cifique d'un site de liaison sur la mol\u00e9cule cible, est coupl\u00e9 \u00e0 une matrice de chromatographie inerte. Dans des conditions de liaison appropri\u00e9es, les mol\u00e9cules cibles sont li\u00e9es au ligand d'affinit\u00e9 en fonction de sa sp\u00e9cificit\u00e9. Les solut\u00e9s non li\u00e9s sont lav\u00e9s \u00e0 travers la colonne. Les mol\u00e9cules cibles adsorb\u00e9es sont ensuite d\u00e9sorb\u00e9es et \u00e9lu\u00e9es de la colonne. Une purification de plusieurs milliers de fois peut \u00eatre obtenue gr\u00e2ce \u00e0 la grande s\u00e9lectivit\u00e9 des interactions d'affinit\u00e9.<\/p>

Les r\u00e9sines d'affinit\u00e9 sp\u00e9cifiques \u00e0 un groupe (par exemple Tosoh Bioscience) lient les mol\u00e9cules partageant des caract\u00e9ristiques structurelles sp\u00e9cifiques. Si une plus grande sp\u00e9cificit\u00e9 est requise, il est \u00e9galement possible d'utiliser des ligands ayant une sp\u00e9cificit\u00e9 pr\u00e9cise pour la mol\u00e9cule cible. Par exemple, la phase prot\u00e9ine A a une affinit\u00e9 sp\u00e9cifique pour la r\u00e9gion Fc des immunoglobulines.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Chromatographie d'immunoaffinit\u00e9<\/h3>

La chromatographie d'immunoaffinit\u00e9 est une forme sp\u00e9cialis\u00e9e de chromatographie d'affinit\u00e9, qui utilise un anticorps ou un fragment d'anticorps comme ligand immobilis\u00e9 sur un support solide de mani\u00e8re \u00e0 conserver sa capacit\u00e9 de liaison. La figure 1 pr\u00e9sente un sch\u00e9ma des \u00e9tapes d'une analyse d'immunoaffinit\u00e9 typique : chargement de l'\u00e9chantillon, lavage de la colonne pour \u00e9liminer les composants de la matrice et les impuret\u00e9s, et \u00e9lution du compos\u00e9 cible.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Applications<\/h2>

Les techniques de chromatographie d'affinit\u00e9 et d'immunoaffinit\u00e9 sont applicables dans un grand nombre de disciplines, notamment la biochimie, l'immunochimie, la virologie et la biologie mol\u00e9culaire. En raison de la disponibilit\u00e9 croissante d'une vari\u00e9t\u00e9 d'anticorps, les s\u00e9parations bas\u00e9es sur les techniques d'immunoaffinit\u00e9 sont de plus en plus utilis\u00e9es dans un large \u00e9ventail d'applications impliquant la purification d'\u00e9chantillons biologiques complexes.<\/p>

Les principaux domaines d'application de la chromatographie d'immunoaffinit\u00e9 sont les prot\u00e9ines et les enzymes. La s\u00e9lectivit\u00e9 de la m\u00e9thode peut \u00eatre am\u00e9lior\u00e9e en combinant la pr\u00e9concentration et le pr\u00e9traitement des \u00e9chantillons offerts par les phases d'immunoaffinit\u00e9 avec les capacit\u00e9s de s\u00e9paration de la CLHP en phase invers\u00e9e. La technique peut \u00e9galement \u00eatre utilis\u00e9e avant les analyses MS en prot\u00e9omique.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t

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Colonnes et masses d'affinit\u00e9 Biovanix<\/span><\/h1>

Analyse des AcM Colonne HPLC Prosep A<\/h2>
\u00a0<\/strong><\/td>Mat\u00e9riau de base<\/strong><\/td>Taille des particules<\/strong><\/td>D\u00e9bit<\/strong><\/td>Service de la vie*<\/strong><\/td>Gamme de pH<\/strong><\/td><\/tr>
Prosep A<\/td>Polym\u00e8re<\/td>40um<\/td>0,5-3 ml\/min<\/td>3000 cercles<\/td>2-10<\/td><\/tr>
Prosep A PLUS<\/td>Polym\u00e8re<\/td>20um<\/td>0,5-3 ml\/min<\/td>3000 cercles<\/td>2-10<\/td><\/tr><\/tbody><\/table>

*Le service vie est bas\u00e9 sur des conditions d'analyse.<\/em><\/p><\/div><\/div><\/div>

Biovanix A50 &<\/strong>\u00a0PrA\/G\u00a0<\/strong>Phases d'affinit\u00e9<\/h2>
\u00a0<\/strong><\/td>Mat\u00e9riau de base<\/strong><\/td>Taille des particules<\/strong><\/td>D\u00e9bit<\/strong><\/td>Capacit\u00e9<\/strong><\/td>Gamme de pH<\/strong><\/td><\/tr>
Biovanix Affinity A50\u00a0<\/strong><\/td>Polym\u00e8re<\/td>40um<\/td>0,5-3 ml\/min<\/td>40 mg d'IgG humaine<\/td>2-10<\/td><\/tr>
Biovanix <\/strong>Pr A<\/strong><\/td>Agarose<\/td>90um<\/td>0,5-3 ml\/min<\/td>20 mg d'IgG humaine<\/td>2-12<\/td><\/tr>
Biovanix <\/strong>Pr G<\/strong><\/td>Agarose<\/td>90um<\/td>0,5-3 ml\/min<\/td>20 mg d'IgG humaine<\/td>2-8<\/td><\/tr>
Biovanix <\/strong>Ni-IMAC<\/strong><\/td>Agarose<\/td>90um<\/td>0,5-3 ml\/min<\/td>30 mg Myoglobine<\/td>3-12<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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Des probl\u00e8mes avec la chromatographie en phase liquide ? Contactez-nous directement.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t
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\n\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\tNous contacter<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Affinity Phases Selectivity Affinity phases offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separations can often remove contaminants difficult to eliminate using conventional chromatographic procedures. Mechanism The basis of affinity chromatography is a \u2018lock and key\u2019 type […]<\/p>","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1,49],"tags":[],"class_list":["post-3806","post","type-post","status-publish","format-standard","hentry","category-blog","category-hplc-introduction"],"aioseo_notices":[],"jetpack_featured_media_url":"","uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/fr\/author\/biovanix-com\/"},"uagb_comment_info":19,"uagb_excerpt":"Affinity Phases Selectivity Affinity phases offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separations can often remove contaminants difficult to eliminate using conventional chromatographic procedures. Mechanism The basis of affinity chromatography is a \u2018lock and key\u2019 type\u2026","aioseo_head":"\n\t\t\n\t\n\t\n\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t