{"id":3201,"date":"2024-11-04T02:01:33","date_gmt":"2024-11-04T02:01:33","guid":{"rendered":"https:\/\/biovanix.com\/?post_type=product&#038;p=3201"},"modified":"2025-03-07T14:16:16","modified_gmt":"2025-03-07T14:16:16","slug":"milieu-de-chromatographie-en-agarose-ni-ida","status":"publish","type":"product","link":"https:\/\/biovanix.com\/fr\/product\/milieu-de-chromatographie-en-agarose-ni-ida\/","title":{"rendered":"Milieu de chromatographie en agarose Ni-IDA 6FF"},"content":{"rendered":"<h1>Milieu de chromatographie en agarose Ni-IDA 6FF<\/h1>\n<p>Le milieu Biovanix Agarose est con\u00e7u sur la base de la s\u00e9rie Cytive Sepharose. Il offre une sp\u00e9cificit\u00e9 et une s\u00e9lectivit\u00e9 \u00e9lev\u00e9es pour les s\u00e9parations et purifications biomol\u00e9culaires. La s\u00e9paration par affinit\u00e9 permet souvent d'\u00e9liminer des contaminants difficiles \u00e0 \u00e9liminer par d'autres proc\u00e9dures chromatographiques. Des purifications de plusieurs ordres de grandeur peuvent \u00eatre r\u00e9alis\u00e9es en une seule \u00e9tape.<\/p>\n<ul>\n<li>Milieu de chromatographie d'affinit\u00e9 ch\u00e9lateur Agarose Ni2+ ;<\/li>\n<li>Capacit\u00e9 d'adsorption dynamique \u00e9lev\u00e9e et fonctionnement \u00e0 d\u00e9bit rapide ;<\/li>\n<li>Principalement utilis\u00e9 pour la s\u00e9paration et la purification des prot\u00e9ines du g\u00e9nie g\u00e9n\u00e9tique marqu\u00e9es \u00e0 l'histidine.<\/li>\n<\/ul>\n<h3>Param\u00e8tres<\/h3>\n<table width=\"68%\">\n<tbody>\n<tr>\n<td width=\"43%\"><\/td>\n<td width=\"56%\"><strong>Ni-IDA 6<\/strong>FF<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Matrice<\/strong><\/td>\n<td width=\"56%\">Agarose r\u00e9ticul\u00e9 6%<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Taille moyenne des particules<\/strong><\/td>\n<td width=\"56%\">90\u03bcm<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Groupe modifi\u00e9<\/strong><\/td>\n<td width=\"56%\">-N(CH<sub>2<\/sub>COOH)<sub>2<\/sub>Ni<sup>2+<\/sup><\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Liaison dynamique<\/strong><\/p>\n<p><strong>Capacit\u00e9<\/strong><\/td>\n<td width=\"56%\">40 mg His\/mL<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Concentration du ligand<\/strong><\/td>\n<td width=\"56%\">15 \u03bcmol\/mL r\u00e9sine<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>pH<\/strong> <strong>Stabilit\u00e9, op\u00e9rationnel<\/strong><\/td>\n<td width=\"56%\">3-13<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>pH<\/strong> <strong>Stabilit\u00e9, CIP<\/strong><\/td>\n<td width=\"56%\">2-14<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Pression<\/strong><\/td>\n<td width=\"56%\">\u22640.3MPa<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Temp\u00e9rature de fonctionnement<\/strong><\/td>\n<td width=\"56%\">4-40\u2103<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Thermostabilit\u00e9<\/strong><\/td>\n<td width=\"56%\">120\u2103\uff0c30min, pH 7<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>D\u00e9bit<\/strong><\/td>\n<td width=\"56%\">600 cm\/h<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Stabilit\u00e9 chimique<\/strong><\/td>\n<td width=\"56%\">Tous les tampons courants, hydroxyde de sodium 1,0M, ur\u00e9e 8,0M, chlorhydrate de guanidine 6,0M, alcool isopropylique 30%, \u00e9thanol 70%<\/td>\n<\/tr>\n<tr>\n<td width=\"43%\"><strong>Stockage<\/strong><\/td>\n<td width=\"56%\">20% EtOH, 4-30\u2103<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 1.5em; font-weight: bold;\">Manuel du produit<\/span><\/p>\n<p>Ni-IDA 6FF<\/p>","protected":false},"excerpt":{"rendered":"<p>Ni-IDA 6FF Agarose Chromatography Media Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step. 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6FF Agarose Chromatography Media Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step.\u2026","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"BioVanix Ni-IDA 6FF Agarose Chromatography Media is based on high-quality agarose particles with Ni IDA ligand. Factory Supply. 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