{"id":4878,"date":"2025-02-08T13:13:16","date_gmt":"2025-02-08T13:13:16","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4878"},"modified":"2025-02-08T15:04:24","modified_gmt":"2025-02-08T15:04:24","slug":"%ec%98%ac%eb%a6%ac%ea%b3%a0-dt25-%ec%b9%9c%ed%99%94%ec%84%b1-%ed%81%ac%eb%a1%9c%eb%a7%88%ed%86%a0%ea%b7%b8%eb%9e%98%ed%94%bc-%ec%88%98%ec%a7%80","status":"publish","type":"page","link":"https:\/\/biovanix.com\/ko\/oligo-dt25-affinity-chromatography-resin\/","title":{"rendered":"Oligo dT(25) \uce5c\ud654\uc131 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub798\ud53c \ub808\uc9c4"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"4878\" class=\"elementor elementor-4878\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f2b3486 e-flex e-con-boxed e-con e-parent\" data-id=\"f2b3486\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-1af111e elementor-widget elementor-widget-heading\" data-id=\"1af111e\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\uc62c\ub9ac\uace0 dT(25) \uce5c\ud654\uc131 \ud06c\ub85c\ub9c8\ud1a0\uadf8\ub798\ud53c \uc218\uc9c0<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6964417 elementor-widget elementor-widget-text-editor\" data-id=\"6964417\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>\ubc14\uc774\uc624\ubc14\ub2c9\uc2a4 \uc62c\ub9ac\uace0 dT(25) \uce5c\ud654\uc131 \uc218\uc9c0<\/strong>\u00a0\uc774\uac83\uc740 \uccb4\uc678 \uc804\uc0ac(IVT) \uc81c\uc870 \uacf5\uc815\uc5d0\uc11c mRNA\uc758 \uc815\uc81c \ubc0f \ubd84\ub9ac\ub97c \uc704\ud574 \uc124\uacc4\ub41c \ucd5c\ucca8\ub2e8 \uc194\ub8e8\uc158\uc785\ub2c8\ub2e4. \uc774 \uace0\uae09 \uc218\uc9c0\ub294 \ud3f4\ub9ac\uc544\ub370\ub2d0\ud654(polyA) \uaf2c\ub9ac\ub97c \ud1b5\ud574 mRNA\ub97c \uc120\ud0dd\uc801\uc73c\ub85c \ud3ec\uc9d1\ud558\uc5ec \ud6a8\uc18c \ubc0f \ud50c\ub77c\uc2a4\ubbf8\ub4dc DNA\uc640 \uac19\uc740 \uc804\uc0ac \ubc18\uc751\uc758 \ub2e4\ub978 \uad6c\uc131 \uc694\uc18c\uc640 \ud6a8\uacfc\uc801\uc73c\ub85c \ubd84\ub9ac\ud569\ub2c8\ub2e4.<\/p><p>\ub300\uaddc\ubaa8 \ud558\ub958 \uc815\uc81c\uc5d0 \uc644\ubcbd\ud558\uac8c \uc801\ud569\ud558\uba70,\u00a0<strong>\ubc14\uc774\uc624\ubc14\ub2c9\uc2a4 \uc62c\ub9ac\uace0 dT(25) \uce5c\ud654\uc131 \uc218\uc9c0<\/strong>\u00a0\ubc31\uc2e0 \ubc0f \uc720\uc804\uc790 \uce58\ub8cc \uc560\ud50c\ub9ac\ucf00\uc774\uc158\uc758 \ud575\uc2ec \ub3d9\ub825\uc73c\ub85c, \ud604\ub300 \ubc14\uc774\uc624 \uc81c\uc57d \uc81c\uc870\uc758 \uc694\uad6c\ub97c \ucda9\uc871\uc2dc\ud0a4\ub294 \ud0c1\uc6d4\ud55c \uc120\ud0dd\uc131\uacfc \uc6a9\ub7c9\uc744 \uc81c\uacf5\ud569\ub2c8\ub2e4.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-63335b8 e-flex e-con-boxed e-con e-parent\" data-id=\"63335b8\" data-element_type=\"container\" data-e-type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b528e69 elementor-widget elementor-widget-text-editor\" data-id=\"b528e69\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2>\uae30\ub2a5<\/h2><h4><strong>\uc120\ud0dd\uc801 mRNA \ud3ec\uc9d1<\/strong><\/h4><ul><li><strong>\ud3f4\ub9ac\uc544 \uaf2c\ub9ac \ud2b9\uc774\uc131<\/strong>\u00a0\ud50c\ub77c\uc2a4\ubbf8\ub4dc DNA \ubc0f \uae30\ud0c0 IVT \uad6c\uc131 \uc694\uc18c\ub97c \ud6a8\uacfc\uc801\uc73c\ub85c \uc81c\uac70\ud558\uae30 \uc704\ud574 mRNA\ub97c \ub192\uc740 \uc120\ud0dd\uc131\uc73c\ub85c \ud45c\uc801\ud654\ud569\ub2c8\ub2e4.<\/li><li><strong>\uac04\ub2e8\ud55c \uc5fc\uc218 \uc6a9\ub9ac<\/strong>\u00a0\ubcf5\uc7a1\uc131\uc744 \ucd5c\uc18c\ud654\ud55c \uac04\uc18c\ud654\ub41c \uc815\uc81c \uacf5\uc815.<\/li><\/ul><h4><strong>2. \ud655\uc7a5 \uac00\ub2a5\ud55c \uc194\ub8e8\uc158<\/strong><\/h4><ul><li><strong>\ud0c1\uc6d4\ud55c \ud655\uc7a5\uc131<\/strong>\u00a0\uc2e4\ud5d8\uc2e4 \uaddc\ubaa8\uc5d0\uc11c \ub300\uaddc\ubaa8 \uc0dd\uc0b0\uc73c\ub85c\uc758 \uc6d0\ud65c\ud55c \uc804\ud658\uc744 \uc704\ud574 \uc124\uacc4\ub418\uc5c8\uc2b5\ub2c8\ub2e4.<\/li><li><strong>\ub300\uc6a9\ub7c9<\/strong>\u00a0\uace0\uc218\uc728 mRNA \uc81c\uc870 \uc694\uad6c \uc0ac\ud56d\uc744 \ucda9\uc871\ud569\ub2c8\ub2e4.<\/li><\/ul><h4><strong>3. \ube44\ub3d9\ubb3c \uc720\ub798<\/strong><\/h4><ul><li><strong>\ud569\uc131 \uc81c\uc870 \uacf5\uc815<\/strong>\u00a0\ubc14\uc774\uc624\ubc14\ub2c9\uc2a4 \uc62c\ub9ac\uace0 \ub9ac\uac04\ub4dc\ub294 \ub3d9\ubb3c \uc131\ubd84\uc774 \ud3ec\ud568\ub418\uc5b4 \uc788\uc9c0 \uc54a\uc544 \uaddc\uc81c \uc694\uad6c \uc0ac\ud56d \ubc0f \uc724\ub9ac\uc801 \uae30\uc900\uc744 \uc900\uc218\ud569\ub2c8\ub2e4.<\/li><\/ul><h4><strong>4. \uac04\uc18c\ud654\ub41c \uc6cc\ud06c\ud50c\ub85c<\/strong><\/h4><ul><li><strong>\ub2e8\uc21c\ud654<\/strong>\u00a0\uc5ec\ub7ec \uc815\uc81c \ub2e8\uacc4\ub97c \uc5c6\uc560 \ud6a8\uc728\uc131\uc744 \uadf9\ub300\ud654\ud558\uace0 \ud6c4\ucc98\ub9ac \ubcf5\uc7a1\uc131\uc744 \uc904\uc785\ub2c8\ub2e4.<\/li><li><strong>\uc77c\uad00\ub41c \uc131\ub2a5<\/strong>\u00a0\ub2e4\uc591\ud55c \uaddc\ubaa8\uc640 \uc0dd\uc0b0 \ubc30\uce58\uc5d0 \uac78\uccd0 \uc2e0\ub8b0\ud560 \uc218 \uc788\ub294 \uacb0\uacfc.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-bd1a46c e-flex e-con-boxed e-con e-parent\" data-id=\"bd1a46c\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-be95407 elementor-widget elementor-widget-text-editor\" data-id=\"be95407\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-row fl-row-fixed-width fl-row-bg-color fl-node-60f5336b77e5a fl-row-default-height fl-row-align-center nitro-offscreen\" data-node=\"60f5336b77e5a\"><div class=\"fl-row-content-wrap\"><div class=\"fl-row-content fl-row-fixed-width fl-node-content\"><div class=\"fl-col-group fl-node-60f5336b7a7d0\" data-node=\"60f5336b7a7d0\"><div class=\"fl-col fl-node-60f5336b7a866 fl-col-bg-color\" data-node=\"60f5336b7a866\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-rich-text fl-node-60f5336b77c2b\" data-node=\"60f5336b77c2b\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><h2><strong>\uc0ac\uc591<\/strong><\/h2><table width=\"627\"><tbody><tr><td width=\"172\"><strong>\ud2b9\uc131<\/strong><\/td><td width=\"455\"><strong>\uc124\uba85<\/strong><\/td><\/tr><tr><td width=\"172\"><strong>\uc9c0\uc6d0 \ud589\ub82c<\/strong><\/td><td width=\"455\">\uac00\uad50 \ud3f4\ub9ac(\uc2a4\ud0c0\uc774\ub80c-\ub514\ube44\ub2d0\ubca4\uc820)<\/td><\/tr><tr><td width=\"172\"><strong>\ud3c9\uade0 \uc785\uc790 \ud06c\uae30<\/strong><\/td><td width=\"455\">50 \ub9c8\uc774\ud06c\ub85c\ubbf8\ud130<\/td><\/tr><tr><td width=\"172\"><strong>\ud3c9\uade0 \uae30\uacf5 \ud06c\uae30<\/strong><\/td><td width=\"455\">200 nm<\/td><\/tr><tr><td width=\"172\"><strong>\ud45c\uba74 \uae30\ub2a5<\/strong><\/td><td width=\"455\">\ud3f4\ub9ac(dT) 25 \uc0c1\uc704 \uc5fc\uae30 \uc11c\uc5f4\uc5d0 \ub3c5\uc810\uc801\uc778 \ub9c1\ucee4<\/td><\/tr><tr><td width=\"172\"><strong>\ub9ac\uac04\ub4dc \ubc00\ub3c4<\/strong><\/td><td width=\"455\">0.3 umol\/ml<\/td><\/tr><tr><td width=\"172\"><strong>\uae30\uacc4\uc801 \ub0b4\uc131<\/strong><\/td><td width=\"455\">70 \ubc14 (1,000 psi; 7 MPa)<\/td><\/tr><tr><td width=\"172\"><strong>\uc5f4 \uc548\uc815\uc131<\/strong><\/td><td width=\"455\">\ud544\uc694\uc2dc 65\u00b0C\uc5d0\uc11c \uc2dc\ub8cc \ubcc0\uc131 \uac00\ub2a5<\/td><\/tr><tr><td width=\"172\"><strong>pH \ubc94\uc704<\/strong><\/td><td width=\"455\">2-13<\/td><\/tr><tr><td width=\"172\"><strong>\uc774\uc628 \uac15\ub3c4 \ubc94\uc704<\/strong><\/td><td width=\"455\">0~5 M, \ubaa8\ub4e0 \uc77c\ubc18 \uc5fc<\/td><\/tr><tr><td width=\"172\"><strong>\ub0b4\ud654\ud559\uc131<\/strong><\/td><td width=\"455\">mRNA \uc815\uc81c\uc5d0 \uc77c\ubc18\uc801\uc73c\ub85c \uc0ac\uc6a9\ub418\ub294 \uc2dc\uc57d\uc73c\ub85c\ub294 0.5 M NaOH, 2 M MgCl\u2082, 20 mM EDTA \ub4f1\uc774 \uc788\uc2b5\ub2c8\ub2e4. \ubb3c, 0~100% \uc54c\ucf54\uc62c, \uc544\uc138\ud1a0\ub2c8\ud2b8\ub9b4, 2 M \ucd08\uc0b0, 1 M HCl \ubc0f \uae30\ud0c0 \uc77c\ubc18\uc801\uc778 \uc720\uae30 \uc6a9\ub9e4\ub3c4 \ud3ec\ud568\ub429\ub2c8\ub2e4.<\/td><\/tr><tr><td width=\"172\"><strong>\uc2a4\ud1a0\ub9ac\uc9c0<\/strong><\/td><td width=\"455\">18-20% \uc5d0\ud0c4\uc62c<\/td><\/tr><\/tbody><\/table><h3>\u00a0<\/h3><h3><strong>\ud654\ud559 \ubc0f \ub0b4\uc5f4\uc131<\/strong><\/h3><table><tbody><tr><td width=\"148\"><strong>pH \ubc94\uc704<\/strong><\/td><td width=\"399\">2\u201313<\/td><\/tr><tr><td width=\"148\"><strong>\uc774\uc628 \uac15\ub3c4 \ubc94\uc704<\/strong><\/td><td width=\"399\">0~5 M, \ubaa8\ub4e0 \uc77c\ubc18 \uc5fc<\/td><\/tr><tr><td width=\"148\"><strong>\uc644\ucda9 \ucca8\uac00\uc81c<\/strong><\/td><td width=\"399\">mRNA \uc815\uc81c\uc6a9 \uc77c\ubc18\uc801\uc778 \uc2dc\uc57d\uc73c\ub85c\ub294 0.5 M NaOH, 2 mM MgCl2, 20 mM EDTA\uac00 \uc788\uc2b5\ub2c8\ub2e4.<p>\u00a0<\/p><p>\uac15\uc0b0\ud654\uc81c(\ucc28\uc544\uc5fc\uc18c\uc0b0\uc5fc \ub4f1), \uc0b0\ud654\uc0b0(\uc9c8\uc0b0 \ub4f1), \uac15\ud658\uc6d0\uc81c(\uc544\ud669\uc0b0\uc5fc \ub4f1), \uc544\uc138\ud1a4, THF \ub610\ub294 \ubca4\uc9c8 \uc54c\ucf54\uc62c\uacfc \uac19\uc740 \ubb3c\uc9c8\uc5d0 \ub178\ucd9c\uc2dc\ud0a4\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/p><\/td><\/tr><tr><td width=\"148\"><strong>\uc6a9\ub9e4<\/strong><\/td><td width=\"399\">\ubb3c, 0~100% \uc54c\ucf54\uc62c, \uc544\uc138\ud1a0\ub2c8\ud2b8\ub9b4, 2 M \ucd08\uc0b0, 1 M \uc5fc\uc0b0 \ubc0f \uae30\ud0c0 \uc77c\ubc18\uc801\uc778 \uc720\uae30 \uc6a9\ub9e4.<p>\u00a0<\/p><p>\uac15\ud55c \uc0b0\ud654\uc81c(\ucc28\uc544\uc5fc\uc18c\uc0b0\uc5fc \ub4f1), \uc0b0\ud654\uc131 \uc0b0(\uc9c8\uc0b0 \ub4f1), \uac15\ud55c \ud658\uc6d0\uc81c(\uc544\ud669\uc0b0\uc5fc \ub4f1), \uc544\uc138\ud1a4, \ubca4\uc9c8 \uc54c\ucf54\uc62c \ub610\ub294 THF\uc5d0 \ub178\ucd9c\uc2dc\ud0a4\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/p><\/td><\/tr><tr><td width=\"148\"><strong>\uc720\ub7c9<\/strong><\/td><td width=\"399\">\uc131\ub2a5\uc5d0 \ub530\ub77c \uc720\ub7c9\uc744 \uc870\uc808\ud558\uc2ed\uc2dc\uc624. \uc0c1\ud55c \uc555\ub825 \uc81c\ud55c\uc744 \ucd08\uacfc\ud558\uc9c0 \ub9c8\uc2ed\uc2dc\uc624.<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7f8781d e-flex e-con-boxed e-con e-parent\" data-id=\"7f8781d\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f583519 elementor-widget elementor-widget-text-editor\" data-id=\"f583519\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><strong>\ud61c\ud0dd \uc694\uc57d<\/strong><\/h2><h4><strong>\uace0\ud6a8\uc728<\/strong><\/h4><p>\ube60\ub974\uace0 \ud6a8\uc728\uc801\uc778 mRNA \uc815\uc81c.<\/p><h4><strong>\ud655\uc7a5 \uac00\ub2a5\ud55c \ud504\ub85c\uc138\uc2a4<\/strong><\/h4><p>\ub300\uaddc\ubaa8 \uc0dd\uc0b0\uc5d0 \uc27d\uac8c \uc801\uc6a9 \uac00\ub2a5.<\/p><h4><strong>\uaddc\uc815 \uc900\uc218<\/strong><\/h4><p>\ub3d9\ubb3c \uc720\ub798\uac00 \uc544\ub2cc \ud569\uc131 \uc81c\uc870 \uacf5\uc815.<\/p><h4><strong>\ube44\uc6a9 \ud6a8\uc728\uc801\uc778<\/strong><\/h4><p>\uac04\uc18c\ud654\ub41c \uc6cc\ud06c\ud50c\ub85c\ub294 \uc6b4\uc601 \ubcf5\uc7a1\uc131\uacfc \ube44\uc6a9\uc744 \uc904\uc785\ub2c8\ub2e4.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-b527727 e-flex e-con-boxed e-con e-parent\" data-id=\"b527727\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-f312b27 elementor-widget elementor-widget-text-editor\" data-id=\"f312b27\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><strong>\uc560\ud50c\ub9ac\ucf00\uc774\uc158<\/strong><\/h2><h4><strong>\ubc31\uc2e0 \uc0dd\uc0b0<\/strong><\/h4><p>mRNA \ubc31\uc2e0 \ud6a8\uc728\uc801\uc778 \uc815\uc81c.<\/p><h4><strong>\uc720\uc804\uc790 \uce58\ub8cc<\/strong><\/h4><p>\uce58\ub8cc \uc6a9\ub3c4 mRNA\uc758 \ud655\uc7a5 \uac00\ub2a5\ud55c \uc0dd\uc0b0.<\/p><h4><strong>IVT \uc81c\uc870<\/strong><\/h4><p>\uc804\uc0ac \ubc18\uc751 \ud63c\ud569\ubb3c\uc5d0\uc11c mRNA\ub97c \uac04\uc18c\ud654\ub41c \ubd84\ub9ac.<\/p><h4><strong>\ud50c\ub77c\uc2a4\ubbf8\ub4dc DNA \uc81c\uac70<\/strong><\/h4><p>\ud50c\ub77c\uc2a4\ubbf8\ub4dc DNA \ubc0f \uae30\ud0c0 IVT \uad6c\uc131 \uc694\uc18c\uc758 \ud6a8\uacfc\uc801\uc778 \uc81c\uac70.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7c1e95b e-flex e-con-boxed e-con e-parent\" data-id=\"7c1e95b\" data-element_type=\"container\" data-e-type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-36532a3 elementor-widget elementor-widget-text-editor\" data-id=\"36532a3\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2><strong>\ubc14\uc774\uc624\ubc14\ub2c9\uc2a4 \uc62c\ub9ac\uace0 dT(25) \uce5c\ud654\uc131 \uc218\uc9c0\ub97c \uc120\ud0dd\ud558\ub294 \uc774\uc720\ub294 \ubb34\uc5c7\uc778\uac00\uc694?<\/strong><\/h2><h4><strong>\ud6a8\uc728\uc801\uc778 mRNA \uc815\uc81c<\/strong><\/h4><p>\ubd88\uc21c\ubb3c\uc744 \ucd5c\uc18c\ud654\ud558\uc5ec 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