{"id":4865,"date":"2025-02-08T06:38:22","date_gmt":"2025-02-08T06:38:22","guid":{"rendered":"https:\/\/biovanix.com\/?page_id=4865"},"modified":"2025-02-08T06:42:49","modified_gmt":"2025-02-08T06:42:49","slug":"protein-a50-psdvb-affinity-media","status":"publish","type":"page","link":"https:\/\/biovanix.com\/ru\/protein-a50-psdvb-affinity-media\/","title":{"rendered":"Protein A50 PSDVB Affinity Media"},"content":{"rendered":"<div data-elementor-type=\"wp-page\" data-elementor-id=\"4865\" class=\"elementor elementor-4865\" data-elementor-post-type=\"page\">\n\t\t\t\t<div class=\"elementor-element elementor-element-873fbec e-flex e-con-boxed e-con e-parent\" data-id=\"873fbec\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-0296c3c elementor-widget elementor-widget-heading\" data-id=\"0296c3c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h1 class=\"elementor-heading-title elementor-size-default\">Protein A50 PSDVB affinity chromatography resin<\/h1>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53bd545 elementor-widget elementor-widget-text-editor\" data-id=\"53bd545\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).<br \/>The ligand of Protein A50 is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS\/DVB microsphere substrate. The rProtein A has a better alkali-resisting ability that ensures stability in high pH conditions. With our hydrophilic treatment and binding technology, we eliminated non-specific binding PS-DVB surface. Hence, Protein A50 is extremely useful for the purification process of monoclonal antibodies.<\/p><p><strong>Advantages\uff1a<\/strong><\/p><ul><li>High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing<\/li><li>Outstanding high dynamic binding capacity at low residence time reduces process time and the amount of medium used<\/li><li>Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP<\/li><li>Low ligand leakage ensures a long lifetime<\/li><li class=\"nitro-offscreen\">High price-performance ratio<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3db3010 e-flex e-con-boxed e-con e-parent\" data-id=\"3db3010\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b5fa2a0 elementor-widget elementor-widget-heading\" data-id=\"b5fa2a0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\u041f\u0440\u0438\u043b\u043e\u0436\u0435\u043d\u0438\u044f<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-23ee177 elementor-widget elementor-widget-text-editor\" data-id=\"23ee177\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul><li>Purification of recombinant proteins.<\/li><li>Separation of enzymes and antibodies.<\/li><li>Capture of His-Tag, GST, and other fusion proteins.<\/li><li>Large-scale biopharmaceutical production.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-213570e e-flex e-con-boxed e-con e-parent\" data-id=\"213570e\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-ee27bea elementor-widget elementor-widget-heading\" data-id=\"ee27bea\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Main\u00a0Characteristics<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f3360bb elementor-widget elementor-widget-text-editor\" data-id=\"f3360bb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"fl-row fl-row-fixed-width fl-row-bg-color fl-node-5dd0ec099131e fl-row-default-height fl-row-align-center nitro-offscreen\" data-node=\"5dd0ec099131e\"><div class=\"fl-row-content-wrap\"><div class=\"fl-row-content fl-row-fixed-width fl-node-content\"><div class=\"fl-col-group fl-node-5dd0ec0993e26\" data-node=\"5dd0ec0993e26\"><div class=\"fl-col fl-node-5dd0ec0993ece fl-col-bg-color\" data-node=\"5dd0ec0993ece\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-rich-text fl-node-5dd0eb9979558\" data-node=\"5dd0eb9979558\"><div class=\"fl-module-content fl-node-content\"><div class=\"fl-rich-text\"><div class=\"fusion-text\"><table style=\"font-size: 16px;\" width=\"609\"><tbody><tr><td width=\"200\"><b>\u041c\u0430\u0442\u0440\u0438\u0446\u0430 \u043f\u043e\u0434\u0434\u0435\u0440\u0436\u043a\u0438<\/b><\/td><td width=\"409\">Poly(styrene\/divinylbenzene) (PS-DVB)<\/td><\/tr><tr><td width=\"200\"><b>\u041b\u0438\u0433\u0430\u043d\u0434<\/b><\/td><td width=\"409\">Recombinant Protein A<\/td><\/tr><tr><td width=\"200\"><b>Ave.\u00a0<\/b><b>Particle size<\/b><\/td><td width=\"409\">50\u00b5m<\/td><\/tr><tr><td width=\"200\"><b>\u0414\u0438\u043d\u0430\u043c\u0438\u0447\u0435\u0441\u043a\u0430\u044f \u0441\u0432\u044f\u0437\u044b\u0432\u0430\u044e\u0449\u0430\u044f \u0441\u043f\u043e\u0441\u043e\u0431\u043d\u043e\u0441\u0442\u044c<\/b>\u00a0<b>(<\/b><b>DBC)<\/b><\/td><td width=\"409\">Approx. 40 mg human IgG\/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm\/h in a column with a bed height of 5 cm, Residence time 0.6 min)<\/td><\/tr><tr><td width=\"200\"><b>Shrinkage\/Swelling<\/b><\/td><td width=\"409\">&lt; 1% from 1-100% organic solvent<\/td><\/tr><tr><td width=\"200\"><b>pH range (Long term)<\/b><\/td><td width=\"409\">pH 2-10<\/td><\/tr><tr><td width=\"200\"><b>Maximum Operating Pressure<\/b><\/td><td width=\"409\">1500 psi (100 bar \/ 10 MPa)<\/td><\/tr><tr><td width=\"200\"><b>Cleaning Agents<\/b><\/td><td width=\"409\">0.1-0.5M NaOH<\/td><\/tr><tr><td width=\"200\"><b>Temperature Stability<\/b><\/td><td width=\"409\">4-40 \u00b0C<\/td><\/tr><tr><td width=\"200\"><b>Delivery Conditions<\/b><\/td><td width=\"409\">20% ethanol (2-8\u2103)<\/td><\/tr><\/tbody><\/table><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div><div class=\"fl-row fl-row-fixed-width fl-row-bg-none fl-node-60ebf4829784c fl-row-default-height fl-row-align-center nitro-offscreen\" data-node=\"60ebf4829784c\"><div class=\"fl-row-content-wrap\"><div class=\"fl-row-content fl-row-fixed-width fl-node-content\"><div class=\"fl-col-group fl-node-60ebf482b3ab5\" data-node=\"60ebf482b3ab5\"><div class=\"fl-col fl-node-60ebf482b3b68 fl-col-bg-color\" data-node=\"60ebf482b3b68\"><div class=\"fl-col-content fl-node-content\"><div class=\"fl-module fl-module-spacer-gap fl-node-60ebf48296c05\" data-node=\"60ebf48296c05\"><div class=\"fl-module-content fl-node-content\">\u00a0<\/div><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-a0bb558 e-flex e-con-boxed e-con e-parent\" data-id=\"a0bb558\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t<div class=\"elementor-element elementor-element-285d2c1 elementor-widget elementor-widget-heading\" data-id=\"285d2c1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Why Choose PSDVB Affinity Chromatography Resin?<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b43c658 elementor-widget elementor-widget-text-editor\" data-id=\"b43c658\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><strong>1. Particle Size<\/strong><\/h3><ul><li><strong>Granule Size:<\/strong>\u00a030 \u03bcm \u2013 100 \u03bcm (customizable).<\/li><li><strong>Uniform Distribution:<\/strong>\u00a0Ensures consistent flow and high-resolution separation.<\/li><\/ul><h3><strong>2. Porous Structure<\/strong><\/h3><ul><li><strong>Pore Size:<\/strong>\u00a0Optimized for target protein size, ensuring efficient mass transfer.<\/li><li><strong>High Porosity:<\/strong>\u00a0Maximizes surface area and loading capacity.<\/li><\/ul><h3><strong>3. Surface Chemistry<\/strong><\/h3><ul><li><strong>High Surface Area:<\/strong>\u00a0BET surface area &gt; 500 m\u00b2\/g.<\/li><li><strong>Minimal Non-Specific Binding:<\/strong>\u00a0Reduced background interference for cleaner elution.<\/li><\/ul><h3><strong>4. Affinity Ligand Properties<\/strong><\/h3><ul><li><strong>Ligand Types:<\/strong>\u00a0Customizable options (e.g., His-Tag, MBP, Strep Tag).<\/li><li><strong>Ligand Density:<\/strong>\u00a0&gt;10 \u03bcmol\/mL, ensuring high binding capacity.<\/li><\/ul><h3><strong>5. Loading Capacity<\/strong><\/h3><ul><li><strong>Dynamic Binding Capacity:<\/strong>\u00a0Up to 50 mg\/mL under high-flow conditions.<\/li><li><strong>High Yield:<\/strong>\u00a0Consistent and reliable protein recovery.<\/li><\/ul><h3><strong>6. Mechanical Strength<\/strong><\/h3><ul><li><strong>High Crush Strength:<\/strong>\u00a0&gt;10 MPa, ensuring long-term stability under pressure.<\/li><\/ul><h3><strong>7. Chemical Durability<\/strong><\/h3><ul><li><strong>pH Stability:<\/strong>\u00a0Operates effectively in a wide pH range (2\u201312).<\/li><li><strong>Resistance to Organic Solvents:<\/strong>\u00a0Stable in high concentrations of acetonitrile and ethanol.<\/li><\/ul><h3><strong>8. Thermal Stability<\/strong><\/h3><ul><li><strong>Heat Resistance:<\/strong>\u00a0Stable at temperatures up to 80\u00b0C, enabling efficient cleaning and sterilization.<\/li><\/ul><h3><strong>9. Batch-to-Batch Consistency<\/strong><\/h3><ul><li><strong>Uniform Performance:<\/strong>\u00a0Ensures consistent separation efficiency across different production batches.<\/li><\/ul><h3><strong>10. Cost Efficiency<\/strong><\/h3><ul><li><strong>Economical Use:<\/strong>\u00a0High loading capacity and long service life reduce overall production costs.<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-16f64849 e-flex e-con-boxed e-con e-parent\" data-id=\"16f64849\" data-element_type=\"container\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-3e827943 e-con-full e-flex e-con e-child\" data-id=\"3e827943\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5be8f86a elementor-widget elementor-widget-text-editor\" data-id=\"5be8f86a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h3><span style=\"color: #ffffff;\">\u041d\u0435 \u043d\u0430\u0448\u043b\u0438 \u043d\u0443\u0436\u043d\u044b\u0439 \u0432\u0430\u043c \u043f\u0440\u043e\u0434\u0443\u043a\u0442? \u0421\u0432\u044f\u0436\u0438\u0442\u0435\u0441\u044c \u0441 \u043d\u0430\u043c\u0438 \u043d\u0430\u043f\u0440\u044f\u043c\u0443\u044e.<\/span><\/h3>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-66a5552 e-con-full e-flex e-con e-child\" data-id=\"66a5552\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4214d054 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"4214d054\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-size-sm\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon\">\n\t\t\t\t<svg aria-hidden=\"true\" class=\"e-font-icon-svg e-far-comment\" viewbox=\"0 0 512 512\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\"><path d=\"M256 32C114.6 32 0 125.1 0 240c0 47.6 19.9 91.2 52.9 126.3C38 405.7 7 439.1 6.5 439.5c-6.6 7-8.4 17.2-4.6 26S14.4 480 24 480c61.5 0 110-25.7 139.1-46.3C192 442.8 223.2 448 256 448c141.4 0 256-93.1 256-208S397.4 32 256 32zm0 368c-26.7 0-53.1-4.1-78.4-12.1l-22.7-7.2-19.5 13.8c-14.3 10.1-33.9 21.4-57.5 29 7.3-12.1 14.4-25.7 19.9-40.2l10.6-28.1-20.6-21.8C69.7 314.1 48 282.2 48 240c0-88.2 93.3-160 208-160s208 71.8 208 160-93.3 160-208 160z\"><\/path><\/svg>\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">\u0421\u0432\u044f\u0436\u0438\u0442\u0435\u0441\u044c \u0441 \u043d\u0430\u043c\u0438<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).The ligand of Protein [&hellip;]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_uag_custom_page_level_css":"","_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_uf_show_specific_survey":0,"_uf_disable_surveys":false,"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"full-width-container","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-4865","page","type-page","status-publish","hentry"],"aioseo_notices":[],"uagb_featured_image_src":{"full":false,"thumbnail":false,"medium":false,"medium_large":false,"large":false,"1536x1536":false,"2048x2048":false,"trp-custom-language-flag":false,"woocommerce_thumbnail":false,"woocommerce_single":false,"woocommerce_gallery_thumbnail":false},"uagb_author_info":{"display_name":"biovanix.com","author_link":"https:\/\/biovanix.com\/ru\/author\/biovanix-com\/"},"uagb_comment_info":0,"uagb_excerpt":"Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).The ligand of Protein&hellip;","aioseo_head":"\n\t\t<!-- All in One SEO Pro 4.9.3 - aioseo.com -->\n\t<meta name=\"description\" content=\"Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).The ligand of Protein\" \/>\n\t<meta name=\"robots\" content=\"max-image-preview:large\" \/>\n\t<link rel=\"canonical\" href=\"https:\/\/biovanix.com\/ru\/protein-a50-psdvb-affinity-media\/\" \/>\n\t<meta name=\"generator\" content=\"All in One SEO Pro (AIOSEO) 4.9.3\" \/>\n\t\t<meta property=\"og:locale\" content=\"ru_RU\" \/>\n\t\t<meta property=\"og:site_name\" content=\"BioVanix | Professional Instrument Supplier\" \/>\n\t\t<meta property=\"og:type\" content=\"article\" \/>\n\t\t<meta property=\"og:title\" content=\"Protein A50 PSDVB Affinity Media | BioVanix\" \/>\n\t\t<meta property=\"og:description\" content=\"Protein A50 PSDVB affinity chromatography resin Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. 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Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. 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