HILIC HPLC Column
Hydrophilic interaction liquid chromatography (HILIC) is a chromatographic technique to improve the retention of very polar substances under reversed-phase chromatography conditions. HILIC has a wide variety of stationary phases, and in principle, any stationary phase with a polar surface can be used in HILIC mode. Therefore, stationary phases such as silica, amino (NH2), diol, amide (AM), and cyanogen (CN) packing materials can also be used as stationary phases for HILIC. HILIC HPLC columns can be used in 100% aqueous phase.
HILIC Column Selection
| Product Name | HILIC Column | ||
| Functional Group | Diol | Amide | Imidazole |
| Substrate | Monodispersed, porous, spherical silica particles | ||
| Particle Size | 3 / 5 μm | ||
| Pore Size | 120 Å | ||
| Surface Area | 300 m2/g | ||
| Carbon Load | 10% | 7% | 5% |
| End-capped | No | ||
| Pressure Limit | 5000 psi for 5 μm 6000 psi for 3 μm |
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| Temperature Limit | 60℃ | ||
| pH Range | 2-8 | ||
| Aqueous Compatibility | 100% aqueous | ||
