ISO Certified Manufacturer

Precision Prepacked HPLC Columns for Unmatched Separation Performance

High-Performance Liquid Chromatography (HPLC) columns are essential tools in modern analytical chemistry, widely used in pharmaceutical development, biomedical research, food safety testing, and environmental monitoring. HPLC columns achieve the efficient separation of complex mixtures by exploiting the differences in the distribution of solutes between the stationary phase and the mobile phase.

10,000

Max Pressure (psi)

2.1–50

Column ID Range (mm)

pH 2–12

Stable Operating Range

20+

Column Types Available

Technical Advantages

Engineered for Reproducibility, High Pressure Resistance & Superior Peak Resolution

Every Biovanix HPLC column is designed to deliver consistent, high-quality separations across a wide range of analytical conditions.

Stationary Phase Types

Common stationary phases include silica, polymers, and chemically modified materials such as C18, C8, NH₂, etc. Different stationary phases are suitable for the separation of compounds with varying polarities.

Particle Size and Pore Size

Particle sizes typically range from 1.8 µm to 10 µm, while pore sizes vary depending on the application, such as 100 Å, 200 Å, and 300 Å. Smaller particles can enhance separation efficiency but may require higher operating pressures.

Column Length and Inner Diameter

The selection of column length and inner diameter depends on the separation requirements. Longer columns generally provide higher separation efficiency.

pH Stability

Some HPLC columns can operate stably over a broad pH range (e.g., pH 2-12), enabling flexible method development with aggressive mobile phases.

Product Catalog

Comprehensive HPLC Column Portfolio

Explore our full range of prepacked chromatography columns organized by application category. Click any product to learn more.

C18 Column & Bulk

C8 Column & Bulk

C4 Column & Bulk

Phenyl Column & Bulk

SiO2 Column & Bulk

NH2/Amino Column & Bulk

CN/Cyano Column & Bulk

Diol Column & Bulk

Amide Column

HILIC Column

Ion-exchange Column

Sugar Column

SEC Column

HIC Column

DNA Column

Chiral Column

Protein A Column

HbA1c Analysis Column

HPLC Column Tubing

PEEK Column Tubing

Guard Column

In-line Filters

Applications

Trusted Across Industries

From drug development to environmental protection, Biovanix HPLC columns deliver reliable performance where it matters most.

Pharmaceutical Development

Used for the separation, purification, and quality control of drug components to ensure the safety and efficacy of pharmaceuticals.

Biomedical Research

Analyzes biomolecules (such as proteins and peptides) and metabolites to support life science research.

Food Safety Testing

Detects harmful substances like additives and pesticide residues in food.

Environmental Monitoring

Monitors organic pollutants and pesticide residues in water and soil.

Selection Guide

How to Choose the Right HPLC Column

Selecting the optimal column dimension and stationary phase is critical for achieving the best separation results.

Depending on the scale and/or efficiency of the separation required, the table below can help you to choose a column by the most appropriate inner diameter (I.D.) and column length for your needs.

Which column length is best for my needs?

If you want to maximize the speed of your application → 20 mm to 75 mm
If you want a balance of resolution and speed → 100 mm
If you want the best resolution possible → 150 mm
Also available in both analytical and semi-prep dimensions

USP (United States Pharmacopeia) columns are high-performance liquid chromatography (HPLC) columns designed to meet the stringent standards set by the USP, ensuring consistency, reliability, and compliance in analytical procedures. These columns are widely used in pharmaceutical, environmental, and food safety applications to provide accurate and reproducible results.

Why Biovanix

Why Choose Biovanix HPLC Columns?

Every column is engineered and manufactured to the highest standards, backed by our commitment to quality and technical support.

01

High-Pressure Stability

Withstands up to 10,000 psi for UHPLC applications

Materials: 316L stainless steel or biocompatible PEEK

02

Exceptional Peak Resolution

Mirror-polished inner surface (Ra ≤0.2 μm) minimizes band broadening, delivering sharp, well-defined peaks for precise quantification.

03

Customizable Options

Column dimensions: 2.1–50 mm ID | 20–500 mm length

Packing materials: C18, C8, HILIC, ion exchange, affinity resins

04

Wide pH Range

Compatible with aggressive mobile phases across a broad pH range, enabling flexible method development for diverse analytes.

05

Low Dead Volume Design

Ensures minimal sample loss and high reproducibility, critical for trace-level analysis and quantitative accuracy.

Need Custom Specifications?

Our engineering team can tailor column dimensions, stationary phases, and end fittings to your exact requirements.

FAQ

Frequently Asked Questions

Everything you need to know about Biovanix prepacked HPLC columns.

What are prepacked HPLC columns and how do they work?

Prepacked HPLC columns are factory-filled, ready-to-use chromatographic columns where the stationary phase (typically chemically modified silica particles) is uniformly packed under high pressure into stainless steel or PEEK tubing.

They work by exploiting differential interactions between analytes, the stationary phase, and the mobile phase. Sample components partition based on hydrophobicity, polarity, charge, or size as the mobile phase is pumped through the column at high pressure. Compounds with stronger affinity for the stationary phase are retained longer, enabling efficient separation, identification, and quantification.

What types of HPLC columns does Biovanix offer?

Biovanix offers six major categories:

Reversed-Phase Columns — C18, C18H, C18L, C18AQ, C18BS, C8, C4-300, Phenyl
Normal-Phase Columns — SiO₂, NH₂, CN, Diol, Amide
Small-Molecule Columns — HILIC, Ion Exchange (SCX/SAX), Sugar Analysis
Biological Columns — SEC (SEC-120/150/300/500/1000), HIC, DNA
Specialty Columns — Chiral (8 types), Protein A, HbA1c
Consumables — SS/PEEK column tubing, guard columns, inline filters, frits, fittings

What is the maximum operating pressure for Biovanix HPLC columns?

Particle Size	Max. Pressure
10 μm	4,000 psi
5 μm	5,000 psi
3 μm	6,000 psi
SEC (3 μm)	2,500 psi
SEC (5 μm)	1,500 psi
UHPLC applications	Up to 10,000 psi

Temperature limits: 60 °C for reversed-phase columns; 40 °C for SEC columns.

Can I customize HPLC column dimensions and packing materials?

Yes. Biovanix supports full customization:

Inner diameter: 2.1–50 mm (10 standard sizes); non-standard sizes available upon request.
Column length: 20–500 mm (8 standard lengths); custom lengths accepted.
Packing materials: C18, C8, Phenyl, NH₂, CN, Diol, Bare Silica and more (particle sizes 3–50 μm; pore sizes 100/120/300 Å). Special bonded phases are available upon request.
Hardware: 316L stainless steel or PEEK tubing; multiple frit types; custom end fittings and seals.

Are Biovanix HPLC columns suitable for pharmaceutical quality control?

Yes. Key qualifications include:

Columns carry recognized USP codes (L1, L3, L7, L8, L9, L10, L11, L14), enabling direct compliance with pharmacopeial methods.
Ultra-pure silica with metal ions < 20 ppm ensures minimal peak tailing for basic compounds.
Proprietary bonding chemistry with deep endcapping delivers low silanol activity and excellent batch-to-batch reproducibility.
pH stability from 2–8 (up to 1–12 for C18BS) covers diverse mobile phase conditions.
Manufacturing follows ISO 9001 quality standards.
Applications explicitly include drug substance characterization, impurity profiling, dissolution testing, and content uniformity analysis.

How do I select the right HPLC column for my application?

Select based on four key parameters:

① Separation mode — Match the analyte properties to the column chemistry:

Hydrophobic compounds → C18 / C8 (reversed-phase)
Aromatic compounds → Phenyl
Polar compounds → HILIC / Amide
Sugars → Sugar-10H / NH₂
Proteins/antibodies → SEC-300 / C4-300
Enantiomers → Chiral columns

② Column dimensions — Choose by scale:

Analytical QC: 2.1–4.6 mm ID × 100–250 mm length
Semi-preparative: 7.8–10 mm ID
Preparative: 20–50 mm ID

③ Particle size — Balance resolution and pressure:

3 μm: highest resolution (pharmacopeial methods)
5 μm: optimal balance (most common)
10 μm: low pressure, scalable to preparative

④ Pore size — Match to molecular weight:

100 Å: small molecules (< 3 kDa)
300 Å: peptides and proteins
SEC series: size-based separation of biomolecules

Ready to Optimize Your Chromatography?

Get detailed pricing, technical specifications, and custom column consultation from our experts.