Ion Exchange IEX HPLC Column
Biovanix ion exchange IEX columns are based on modified silica particles and include weak cation exchange (WCX), strong cation exchange (SCX) and strong anion exchange (SAX) columns.
- WCX columns are carboxylic acid modified silica particles that designed for separating charged variants in proteins, including monoclonal antibodies (mAbs) and related substances.
- SCX columns are based on sulfonate-modified silica particles. These columns feature a strong cation exchange property and are suitable for cationic analytes.
- SAX columns are based on quaternary ammonium modified silica particles that feature a strong anion exchange property and are suitable for separating anionic analytes.
Ion-exchange Column Selection
| Product Name | WCX Column | SCX Column | SAX Column |
| Functional Group | Carboxylic Acid | Sulfonic Acid | Quaternary Ammonium |
| Substrate | Monodispersed spherical PS/DVB particles | ||
| Particle Size | 5 & 10 μm | ||
| Pore Size | Nonporous | ||
| Pressure Limit | 4500 psi for 10 μm 5000 psi for 5 μm |
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| Temperature Limit | 60 ℃ | ||
| pH Range | 2-12 | ||
Application
