DNA Analysis HPLC Column
By liquid chromatography, DNA analysis columns are designed for high-resolution separation of oligonucleotides, including mRNAs, plasmids and short oligos. These columns are based on advanced mono-dispersed particle technology and specially designed column chemistry, resulting in high resolution, ideal selectivity, good stability and decent consistency. DNA column offering consists of three separation modes: RP, SAX, and SEC, providing a complete toolset and application coverage abroad for oligonucleotide separation in biotech, biopharmaceutical, and academic research.
- DNA RP column is based on macroporous PS/DVB microspheres with high crosslinking degrees, and they are suitable for analyzing plasmid topology content.
- DNA 1000-SEC is designed for aggregate and fragment analysis in mRNAs and plasmids
- DNA 1000-C18 is based on monodispersed C18 bonded silica gel for the separation of large oligonucleotides, DNAs and RNAs.
- DNA AAV-SEC is designed for aggregates analysis of AAVs in different serotypes.
- DNA AAV-Q is designed for empty/full ratio analysis of capsid content in different serotypes of AAV.
DNA HPLC Column Selection
Product | DNA RP | DNA 1000–SEC | DNA 1000-C18 | DNA AAV-SEC | DNA AAV-Q |
Functional Group | Quaternary Ammonium | Diol | Octadecyl | Diol | Quaternary ammonium |
Substrate | PS-DVB | Spherical Silica | Spherical Silica | Spherical Silica | PS-DVB |
Particle Size | 5um | 3um | 5um | 3um | 10um |
Pore Size | – | 1000A | 1000A | – | – |
Pressure Limit | 5000 psi | 2500psi | 5000psi | 3000psi | 4000psi |
Temperature Limit | 80℃ | 40℃ | 60℃ | 40℃ | 60℃ |
pH Range | 2-12 | 2-8 | 2-11 | 2-8 | 2-12 |
Application | Content analysis of plasmid topology | Aggregates and fragments analysis in mRNAs and plasmids | Purity analysis of oligonucleotides. Including short-chain oligos, DNA, mRNAs, plasmids, etc | Aggregates analysis of AAVs in different serotypes | Empty/Full capsid content analysis of AAVs in different serotypes |
DNA Column Product
Application
