DNA Analysis HPLC Column

By liquid chromatography, DNA analysis columns are designed for high-resolution separation of oligonucleotides, including mRNAs, plasmids and short oligos. These columns are based on advanced mono-dispersed particle technology and specially designed column chemistry, resulting in high resolution, ideal selectivity, good stability and decent consistency. DNA column offering consists of three separation modes: RP, SAX, and SEC, providing a complete toolset and application coverage abroad for oligonucleotide separation in biotech, biopharmaceutical, and academic research.

  • DNA RP column is based on macroporous PS/DVB microspheres with high crosslinking degrees, and they are suitable for analyzing plasmid topology content.
  • DNA 1000-C18 is based on monodispersed C18 bonded silica gel, is used for the purity analysis of oligonucleotides including short-chain oligos, DNA, mRNA, plasmids, etc.
  • DNA SEC1000 is based on monodispersed silica gel for the aggregates and fragment analysis in mRNAs and plasmids.

DNA HPLC Column Selection

ProductDNA RPDNA 1000-C18DNA SEC1000
Functional GroupQuaternary AmmoniumOctadecylDiol
SubstratePS-DVBSpherical SilicaSpherical Silica
Particle Size5um5um3um
Pore Size1000A1000A
Pressure Limit5000 psi5000psi2500psi
Temperature Limit80℃60℃40℃
pH Range2-122-112-8

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