DNA Analysis HPLC Column
By liquid chromatography, DNA analysis columns are designed for high-resolution separation of oligonucleotides, including mRNAs, plasmids and short oligos. These columns are based on advanced mono-dispersed particle technology and specially designed column chemistry, resulting in high resolution, ideal selectivity, good stability and decent consistency. DNA column offering consists of three separation modes: RP, SAX, and SEC, providing a complete toolset and application coverage abroad for oligonucleotide separation in biotech, biopharmaceutical, and academic research.
- DNA RP column is based on macroporous PS/DVB microspheres with high crosslinking degrees, and they are suitable for analyzing plasmid topology content.
- DNA 1000-C18 is based on monodispersed C18 bonded silica gel, is used for the purity analysis of oligonucleotides including short-chain oligos, DNA, mRNA, plasmids, etc.
- DNA SEC1000 is based on monodispersed silica gel for the aggregates and fragment analysis in mRNAs and plasmids.
DNA HPLC Column Selection
Product | DNA RP | DNA 1000-C18 | DNA SEC1000 |
Functional Group | Quaternary Ammonium | Octadecyl | Diol |
Substrate | PS-DVB | Spherical Silica | Spherical Silica |
Particle Size | 5um | 5um | 3um |
Pore Size | – | 1000A | 1000A |
Pressure Limit | 5000 psi | 5000psi | 2500psi |
Temperature Limit | 80℃ | 60℃ | 40℃ |
pH Range | 2-12 | 2-11 | 2-8 |