Benzamidine 4FF Agarose Chromatography Media
Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step.
- Affinity chromatography mediums for various serine proteases and ligands are broad-spectrum inhibitors of serine proteases;
- High dynamic capacity and fast flow rate operation;
- Mainly used for the separation and purification of trypsin, thrombin, urokinase, kallikrein, pre-kallikrein and other serine proteases.
Parameter
| Benzamidine 4FF | |
| Substrate | 4% cross-linked agarose |
| Ligand | Benzamidine |
| Particle Size | 90µm (45-165µm) |
| Dynamic Binding Capacity | 20 mg trypsin/mL (High Sub)
10 mg trypsin/mL (Low Sub) |
| pH Stability, operational | 2-8 |
| pH Stability, CIP | 1-9 |
| Max. Pressure | 0.3MPa |
| Temperature, operational | 4-40℃ |
| Flow Rate | 500cm/h |
| Chemical Stability | Commonly used aqueous buffer, 8 M urea, 6 M guanidine hydrochloride, 30% isopropyl alcohol |
| Storage | 20% EtOH with 0.05M sodium acetate, pH 4.0 |
