Ni-IDA 6FF Agarose Chromatography Media
Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step.
- Agarose Ni2+ chelating affinity chromatography medium;
- High dynamic adsorption capacity and fast flow rate operation;
- Mainly used for the separation and purification of histidine-labeled genetic engineering proteins.
Parameter
| Ni-IDA 6FF | |
| Matrix | 6% cross-linked Agarose |
| Average Particle Size | 90μm |
| Changed Group | -N(CH2COOH)2Ni2+ |
| Dynamic Binding
Capacity |
40 mg His/mL |
| Ligand Concentration | 15 μmol/mL resin |
| pH Stability, operational | 3-13 |
| pH Stability, CIP | 2-14 |
| Pressure | ≤0.3MPa |
| Temperature, operational | 4-40℃ |
| Thermostability | 120℃，30min, pH 7 |
| Flow Rate | 600 cm/h |
| Chemical Stability | All common buffer, 1.0M sodium hydroxide, 8.0M urea, 6.0M guanidine hydrochloride, 30% isopropyl alcohol, 70% ethanol |
| Storage | 20% EtOH, 4-30℃ |
Product Manual
Ni-IDA 6FF
