Protein A Affinity HPLC Column
For mAb Analysis
mAb analysis HPLC column is used for monoclonal antibody analysis in labs. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions. Protein A affinity chromatography is a fast, accurate, and reliable method. Absolut A is protein A affinity columns for mab analysis.
- Direct use on HPLC instrument
- Fast and accurate analysis of antibody concentration
- Wide linearity range
Technical Parameter
| Ligand | Substrate | Pressure | pH Range | Dynamic Capacity | Flow Rate | Life Time | |
| Pr-A Column | rProtein A | 30 μm PSDVB | 1200psi | 2-11 | 40mg human IgG/ml media* | 1-3ml/min | 3000-5000 circles* |
*PS/DVB: Poly(styrene/divinylbenzene) *The testing result is based on local laboratory tests.
Advantages of Pr-A Column
- Mono-dispersed PS/DVB particles feature very high rigidity, low back pressure, and quick mass transfer.
- Unique hydrophilic surface treatment technology minimizes the nonspecific absorption and guarantees accurate determination of antibody concentration.
- The protein binds specifically to the Fc region of IgG, and the high selectivity guarantees the accuracy of concentration determination.
- Fast analysis cycle time: 2 – 5 minutes.
- Satisfactory linearity within a wide concentration range from 0.02 to 10 mg/ml.
- Long lifetime: 3000-5000 analysis cycles.
- New alkali-resistant Protein A ligand warrants 0.1-0.5M NaOH cleaning conditions.
Product
Pr-A Column
Analysis Condition Of Pr-A Column
| Eluent A | 20-50 mM Phosphate, 150 mM NaCl, pH7-7.5 |
|---|---|
| Eluent B | 12 mM HCl, 150 mM NaCl, pH1.9, or 100 mM Gly-HCl, 150 mM NaCl, pH2.5 |
| Flow rate | 1 mL/min |
| Injection | 1-100 μL |
| Detection | UV 280nm |
Method Timetable
| Time(min) | Gradient(%B) | |
|---|---|---|
| 0.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.01 | 100 | Elution, 2 min, 20 CV |
| 3.00 | 100 | Elution, 2 min, 20 CV |
| 3.01 | 0 | Reequilibration, 2 min, 20 CV |
| 5.00 | 0 | Reequilibration, 2 min, 20 CV |
* The analysis condition varies according to different machines.
