Prosep Protein A Affinity HPLC Column
For mAb HPLC Analysis
Prosep mAb analysis HPLC column is used for monoclonal antibody analysis in laboratories. The determination of IgG concentration (titer) uses for the rapid screening of cell culture samples and optimization of fermentation conditions.
- Direct use on HPLC instrument
- Fast and accurate analysis of antibody concentration
- Wide linearity range
Technical Parameter
|
|
Ligand |
Substrate |
Pressure |
pH Range |
Dynamic Capacity |
Flow Rate |
Life Time |
|
Prosep PrA PLUS Column |
rProtein A |
20 μm PSDVB |
1200psi |
2-11 |
40mg human IgG/ml media[1] |
1-3ml/min |
1000-3000 circles[2] |
|
Prosep PrA Column |
rProtein A |
40 μm PSDVB |
1200psi |
2-11 |
40mg human IgG/ml media[1] |
1-3ml/min |
1000-3000 circles[2] |
- PS/DVB: Poly(styrene/divinylbenzene)
- The testing result is based on local laboratory tests.
Prosep PrA Product
Analysis Condition For Prosep PrA Column
| Eluent A | 20-50 mM Phosphate, 150 mM NaCl, pH7-7.5 |
|---|---|
| Eluent B | 12 mM HCl, 150 mM NaCl, pH1.9, or 100 mM Gly-HCl, 150 mM NaCl, pH2.5 |
| Flow rate | 1 mL/min |
| Injection | 1-100 μL |
| Detection | UV 280nm |
Method Timetable
| Time(min) | Gradient(%B) | |
|---|---|---|
| 0.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.00 | 0 | Equilibration/Wash, 1 min, 10 CV |
| 1.01 | 100 | Elution, 2 min, 20 CV |
| 3.00 | 100 | Elution, 2 min, 20 CV |
| 3.01 | 0 | Reequilibration, 2 min, 20 CV |
| 5.00 | 0 | Reequilibration, 2 min, 20 CV |
* The analysis condition varies according to different machines.
