Protein A50 PSDVB affinity chromatography resin
Protein A50 Affinity Chromatography Media is designed to analyze and purify monoclonal antibodies (mAbs). Compared to traditional agarose media Protein A50 has the advantages of high dynamic binding capacity (DBC), long service life, and less shedding of ligand. NaOH (0.1-0.5M) can be used for clean-in-Place (CIP).
The ligand of Protein A50 is recombinant protein A (rProtein A) immobilized on the surface of the macro-porous PS/DVB microsphere substrate. The rProtein A has a better alkali-resisting ability that ensures stability in high pH conditions. With our hydrophilic treatment and binding technology, we eliminated non-specific binding PS-DVB surface. Hence, Protein A50 is extremely useful for the purification process of monoclonal antibodies.
Advantages：
- High rigidity monodisperse matrix, low back pressure, suitable for large-scale downstream processing
- Outstanding high dynamic binding capacity at low residence time reduces process time and the amount of medium used
- Novel Protein A ligand allows the use of 0.1-0.5 M NaOH for CIP
- Low ligand leakage ensures a long lifetime
- High price-performance ratio
Applications
- Purification of recombinant proteins.
- Separation of enzymes and antibodies.
- Capture of His-Tag, GST, and other fusion proteins.
- Large-scale biopharmaceutical production.
Main Characteristics
| Support Matrix | Poly(styrene/divinylbenzene) (PS-DVB) |
| Ligand | Recombinant Protein A |
| Ave. Particle size | 50µm |
| Dynamic Binding Capacity (DBC) | Approx. 40 mg human IgG/ml media (Determined at 10% breakthrough by frontal analysis at a mobile phase velocity of 500 cm/h in a column with a bed height of 5 cm, Residence time 0.6 min) |
| Shrinkage/Swelling | < 1% from 1-100% organic solvent |
| pH range (Long term) | pH 2-10 |
| Maximum Operating Pressure | 1500 psi (100 bar / 10 MPa) |
| Cleaning Agents | 0.1-0.5M NaOH |
| Temperature Stability | 4-40 °C |
| Delivery Conditions | 20% ethanol (2-8℃) |
Why Choose PSDVB Affinity Chromatography Resin?
1. Particle Size
- Granule Size: 30 μm – 100 μm (customizable).
- Uniform Distribution: Ensures consistent flow and high-resolution separation.
2. Porous Structure
- Pore Size: Optimized for target protein size, ensuring efficient mass transfer.
- High Porosity: Maximizes surface area and loading capacity.
3. Surface Chemistry
- High Surface Area: BET surface area > 500 m²/g.
- Minimal Non-Specific Binding: Reduced background interference for cleaner elution.
4. Affinity Ligand Properties
- Ligand Types: Customizable options (e.g., His-Tag, MBP, Strep Tag).
- Ligand Density: >10 μmol/mL, ensuring high binding capacity.
5. Loading Capacity
- Dynamic Binding Capacity: Up to 50 mg/mL under high-flow conditions.
- High Yield: Consistent and reliable protein recovery.
6. Mechanical Strength
- High Crush Strength: >10 MPa, ensuring long-term stability under pressure.
7. Chemical Durability
- pH Stability: Operates effectively in a wide pH range (2–12).
- Resistance to Organic Solvents: Stable in high concentrations of acetonitrile and ethanol.
8. Thermal Stability
- Heat Resistance: Stable at temperatures up to 80°C, enabling efficient cleaning and sterilization.
9. Batch-to-Batch Consistency
- Uniform Performance: Ensures consistent separation efficiency across different production batches.
10. Cost Efficiency
- Economical Use: High loading capacity and long service life reduce overall production costs.
