SEC Column
BioVanix SEC HPLC column is a family of high-performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of analytes. The column technology involves the creation of a neutral hydrophilic layer on the surface of specially designed high-strength monodispered silica particles followed by a well-established production process. Therefore, SEC columns can be used in pharmaceutical, biopharmaceutical and academic research applications.
Features
- High column efficiency, high resolution;
- Minimal undesired interactions between stationary phase and analytes, resulting in good peak shape and recovery;
- High physical strength for better column lifetime;
- Broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.
Types
- SEC-120 – designed for mall-molecule, drugs, peptides, glycans, and small oligos.
- SEC-150 – designed for separating peptides, glycans, small oligos, and small proteins.
- SEC-300 – designed for mAb aggregate determination.
- SEC-500 – designed for separating larger proteins and oligos.
- SEC-1000 – designed for mAbs and high-order aggregates, large proteins and large DNA/RNA.
Product
SEC-120 Column
SEC-150 Column
SEC-300 Column
SEC-500 Column
Column Selection
| Product Name | SEC-120 | SEC-150 | SEC-300 | SEC-500 |
| Functional Group | Diol | |||
| Substrate | Monodispersed, high pore volume, porous silica particles | |||
| Particle Size | 3 / 5μm | |||
| Pore Size | 120Å | 150Å | 300Å | 500Å |
| Pressure Limit | >2500 psi (3 μm) >1500 psi (5 μm) |
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| Temperature Limit | 40 ℃ | |||
| pH Range | 2-8 | |||
| Calibration Curve（PEG） | 300-10000 | 500-15000 | 1000-50000 | 5000-200000 |
| Calibration Curve（Glucan） | NA | 1000-30000 | 2000-100000 | 20000-500000 |
| Calibration Curve（Globular protein） | NA | 5000-150000 | 10000-750000 | 20000-1500000 |
| Application | Small-molecule drugs, peptides, glycans, small oligos | Small-molecule drugs, peptides, glycans, small oligos and small proteins | mAbs and aggregates | mAbs and high order aggregates, large proteins and large DNA/RNA |
