SEC HPLC Column

BioVanix SEC HPLC column is a family of high-performance, size exclusion chromatography (SEC) columns for separating a broad range of biomolecules based on the size of analytes. The column technology involves the creation of a neutral hydrophilic layer on the surface of specially designed high-strength monodispered silica particles followed by a well-established production process. Therefore, SEC columns can be used in pharmaceutical, biopharmaceutical and academic research applications.

Features

  • High column efficiency, high resolution;
  • Minimal undesired interactions between stationary phase and analytes, resulting in good peak shape and recovery;
  • High physical strength for better column lifetime;
  • Broad range of applications, including small molecule drugs, peptides, proteins, oligos, glycans, etc.

Types

  • SEC-120 – designed for mall-molecule, drugs, peptides, glycans, and small oligos.
  • SEC-150 – designed for separating peptides, glycans, small oligos, and small proteins.
  • SEC-300 – designed for mAb aggregate determination.
  • SEC-500 – designed for separating larger proteins and oligos.
  • SEC-1000 – designed for mAbs and high-order aggregates, large proteins and large DNA/RNA.

SEC Column Selection

Product Name SEC-120 SEC-150 SEC-300 SEC-500
Functional Group Diol
Substrate Monodispersed, high pore volume, porous silica particles
Particle Size 3 / 5μm
Pore Size 120Å 150Å 300Å 500Å
Pressure Limit >2500 psi (3 μm)
>1500 psi (5 μm)
Temperature Limit 40 ℃
pH Range 2-8
Calibration Curve(PEG) 300-10000 500-15000 1000-50000 5000-200000
Calibration Curve(Glucan) NA 1000-30000 2000-100000 20000-500000
Calibration Curve(Globular protein) NA 5000-150000 10000-750000 20000-1500000
Application Small-molecule drugs, peptides, glycans, small oligos Small-molecule drugs, peptides, glycans, small oligos and small proteins mAbs and aggregates mAbs and high order aggregates, large proteins and large DNA/RNA

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