Affinity Chromatography
Agarose
Beads
Agarose beads are the main matrix for affinity chromatography. The products are white suspensions not powder that keeps in 20% alcohol. Biovanix’s affinity chromatography agarose beads are specialized media used to purify targeted proteins of specific biomolecules, leveraging the power of affinity interactions.
- Precision Purification: Our affinity agarose media is designed for the highly specific capture of target biomolecules, based on their unique affinity interactions, ensuring a pure and concentrated product.
- Versatile Ligand Options: Compatible with a wide array of ligands, including antibodies, peptides, and small molecules, allowing for the customization of the purification process to fit specific biomolecular targets.
- High Binding Affinity: Offers exceptional binding affinity, which is crucial for the selective capture of even low-abundance proteins or other biomolecules of interest.
- Gentle Operation Conditions: The mild conditions required for binding and elution with our agarose media are ideal for preserving the integrity and bioactivity of sensitive biomolecules.
- Consistent Quality: Each batch of affinity agarose media is manufactured to the highest quality standards, ensuring consistent performance and reliable results.
- Scalability: Suitable for both small-scale research applications and large-scale industrial production, our media can be easily scaled to meet varying demands.
- Ease of Use: Simple to integrate into existing purification processes, our affinity agarose media streamlines the purification workflow, reducing complexity and enhancing efficiency.
The Selection of agarose beads in affinity chromatography
| Product | Dynamic Binding Capacity | Application |
| Ni-IDA 6 FF | 40 mg His Protein/mL | High loading capacity Isolation and purification of recombinant histidine labeled (His-Tag) proteins |
| Ni-IDA 6 HP | 40 mg His Protein/mL | |
| Ni-NTA 6 FF | 40 mg His Protein/mL | Low Ni2+ leakage Isolation and purification of recombinant histidine labeled (His-Tag) proteins |
| Ni-NTA 6 HP | 40 mg His Protein/mL | |
| Ni-TED 6 FF | 10-15 mg His Protein/mL | It is mainly used for the separation and purification of histidine labeled (His-Tag) genetic engineering proteins containing EDTA or DTT in the feed liquid |
| Ni-TED 6 HP | 10-15 mg His Protein/mL | |
| GSH 4 FF | 10 mg GST /mL | Isolation and purification of glutathione transferase labeled protein (GST fusion protein), glutathione transferase and glutathione dependent protein |
| Blue 6 FF | 18 mg BSA/mL | Isolation and purification of NADH or NADPH coenzyme enzymes (such as dehydrogenase), serum albumin, interferon, thrombin, coagulation factor and other proteins and peptides |
| Blue 6 HP | 20 mg BSA/mL | |
| Benzamidine 4 FF | 40 mg Trypsin /mL | Isolation and purification of serine proteases such as trypsin, thrombin, urokinase, kallikrein and prekallikrein |
| MMA 6 FF | 50 mg BSA/mL | It is widely used in the separation and purification of proteins, especially in the removal of protein A shed from monoclonal antibodies through protein A affinity medium and antibody dimers, host proteins, nucleic acids, viruses, etc |
| MMC 6 FF | 60 mg LZM /mL | Widely used in the separation and purification of proteins |
The Advantages Of Agarose Gel Beads
Agarose gel beads are widely used in affinity chromatography, and their main advantages include:
- Biocompatibility: Agarose is a natural polysaccharide that has good biocompatibility and is less likely to cause denaturation or loss of activity of biomolecules.
- High Porosity: Agarose beads have high porosity, which helps to improve the mass transfer efficiency in the chromatographic process, thereby enhancing the separation efficiency.
- Chemical Stability: Agarose beads are stable over a wide pH range, allowing them to be used in different buffer systems.
- Good Mechanical Strength: Agarose beads possess a certain level of mechanical strength, enabling their use under high flow rates, which is beneficial for increasing production efficiency and reducing costs.
- Easy Functionalization: Agarose can be functionalized through various chemical reactions, facilitating the introduction of affinity ligands, such as protein A, for the purification of specific proteins.
- Cost-Effective: Compared to other types of matrices, agarose is generally less expensive, making it more attractive for industrial-scale chromatographic applications.
- Low non-specific Adsorption: Agarose beads typically have low non-specific adsorption, which helps to reduce impurities in the sample and improve the selectivity of the purification process.
- Reusable: With proper regeneration and cleaning, agarose beads can be reused multiple times, helping to reduce the cost of the purification process.
- Environmentally Friendly: Since agarose is biodegradable, the chromatographic process using agarose beads has a smaller environmental impact.
How to choose the media of affinity chromatography
When selecting affinity chromatography packing materials, several key factors should be considered to ensure that the packing is suitable for specific separation needs. Here are some factors to consider when choosing affinity chromatography packing materials:
- Target Molecule Characteristics: First, consider the size, charge, hydrophobicity, and other biochemical properties of the target molecule, as these will affect its interaction with the packing material.
- Physical and Chemical Stability of the Packing: The packing should have good mechanical and chemical stability to withstand the pressures and different chemical environments of the chromatographic process.
- Porosity and Surface Area: High porosity and a large surface area contribute to improved mass transfer efficiency and target molecule binding capacity.
- Biocompatibility: The packing should have good biocompatibility to minimize non-specific adsorption and denaturation of the target molecule.
- Choice of Affinity Ligand: The affinity ligand should have high specificity and affinity to effectively capture the target molecule. Common affinity ligands include antibodies, protein A, metal ion chelators, lectins, and dyes.
- Cost-Effectiveness: The cost of the packing material is also an important factor, especially in large-scale purification processes.
- Regeneration and Reusability: Ideal packing materials should be capable of being reused over multiple purification cycles without losing performance.
- Flow Rate and Resolution: The packing should allow for rapid flow while maintaining good resolution to effectively separate the target molecule.
- Compatibility: The packing should be compatible with the chromatography system used, including compatibility with the mobile phase and detection systems.
According to the search results, agarose packing materials have become popular in affinity chromatography due to their low cost, large pore size, low non-specific binding, and broad pH stability. However, agarose has limited mechanical stability at high operating pressures, which may limit its use in high-performance liquid chromatography (HPLC) systems. Other carbohydrate-based materials, such as cellulose, have also been used as supports in affinity chromatography, although they were more common in early affinity chromatography applications and are now often used in membrane-based affinity separations.
