Biopure AF Perfusion Chromatography Resin
Combining innovative bonding and surface treatment techniques, Biovanix developed a series of high-performance Biopure perfusion chromatography resins. Biopure is hydrophilic porous polymer microspheres developed based on PSDVB microspheres with extremely low non-specific adsorption. Biopure media are top-performing chromatographic media for process-scale bioseparations. The rigid, robust particles enable high-resolution separations with 2–3x the throughput of conventional fast-flow gels. They are easier to handle and pack and offer outstanding cleanability.
Biopure perfusion chromatography media offers better choices for your purification processes in biological science, biological engineering, and pharmaceutical industry areas.
Virus purification is the basis of developing high-quality vaccines and also provides an important basis for the study of virus fine morphological structure, isolation and purification of virus antigen protein, and detailed studies of virus chemical composition and genetic material.
The sucrose density gradient ultrafast purification process in traditional virus vaccine purification processing requires high-cost large centrifugal devices. These devices with small sample loading, long treatment times, and poor removal of host cell DNA. Gel filtration chromatography, widely used in a vaccine purification process, is mild, repeatable and easy to industrialise. However its efficiency is low, and the effect of removing heteroprotein and DNA is not obvious.
VirCap® AF media is an affinity chromatography media designed to capture and moderate capsular virus purification stages. Specific adsorption of VirCap® AF media and target occurs by simulating the affinity between ligands and virus particles with capsular membranes. With unique high loading capacity, high flow rate and low back pres-sure, VirCap® AF media reduces the process cycle time and increases the yield, fully meeting the requirements of large-scale biopharmaceutical and vaccine production processes.
Biopure AF Media Advantages:
√ High selectivity, similar affinity to live and inactivated capsular viruses.
√ Closed operation, safety, and sterility.
√ Enrichment and purification are carried out simultaneously, increase production, reduce processing steps, time and cost.
√ Rigid particles can be operated under a high flow rate, improve the purification efficiency.
√ Good chemical stability, longer service life.
Mutiple Choices
Ion-exchange, affinity and hydrophobic interactive media. Multiple choices for different application.
Rigid Microsphere With Large Pore Size
The core of VirCap® particles is the novel porous structure with large “throughpores”. These large throughpores allow part of mobile phase flow through, quickly carrying biomolecules to smaller diffusive pores. The large “throuhpores” reduce diffusion rate of biomolecules and enhance interaction between biomolecules and functional groups on the surface. Consequently, diffusion barriers are lowered, and flow rate can be dramatically increased-without compromising capacity or resolution.
- 3000-6000Å pore size, enable the diffusion and mass transfer for large biomolecules.
- 45-90 micron particles, satisfy your purification processing requests.
Flexible Tentacles
- Flexible tentacle structure minimizes the steric hindrance between functional groups and target molecules. It also improves the binding capability of the target material.
- Compared to traditional media, VirCap® media have more effective capture and higher total recovery.
High Flow Rate, Low Backpressure
Biopure media offer an excellent balance of resolution and operating backpressure.
Under the condition of mobile phase change, VirCap® media exhibites almost no shrinkage or expansion. Throughpores and flexible tentacles ensures the rapid diffusion of solute, reduces the barrier of mass transfer, and realizes the high dynamic load under the operation of high flow rate.
Robust Chemical Stability
Biopure media are rigid polymeric particles coated with a proprietary hydrophilic polymer onto which the various functional groups (ion exchange, affinity, etc.) are covalently attached. The result is a highly robust, chemically stable product that is ideally suited for large-scale biopharmaceutical applications.
Clean-in-Place Condition (CIP)
0.5-1M NaOH, organic solvent, high salt solvent.
Biopure AF Resin Parameter
| Substrate | Hydrophilic PS/DVB Microspheres |
| Particle Size | 70um |
| Dynamic Binding Capability | 100mg lysozyme/ml |
| Flow Rate | 1000cm/h (20℃, buffer solution viscosity same as water，pressure < 3 bar / 43.5psi, column bed height 20cm) |
| Column Bed Height | 20-40cm |
| pH Stability | 1-14 |
| Working Temperature | 4-30℃ |
| CIP Condition | 0.5-1M NaOH |
| Storage | 2-8℃ 20% EtOH |
Biopure AF Resin Application
| Viruses | Viral/Microbial Antigens | |
| Rabies | Feline Calicivirus | Herpes Simplex gA and gB Glycoprotein Subunits |
| Influenza | Respiratory Syncytial Virus | Hepatitis B Surface Antigen |
| Japanese Enchephalitis | Human Herpes Simplex | Filamentous Hemagglutinin from B. pertussis |
| Feline Leukemia | Human Measles | Leucocytosis Promoting Factor Hemagglutinin |
| Feline Herpes | Human Parainfluenza | |
Particle SEM Picture
Ligand Bonding Technology
Functional groups or ligand on traditional packing materials are distributed along their surface. This limits their ability to effectively interact with bio-molecules. GALAK developed technologies to build “tentacle structure” on its ion-exchange and affinity packing materials. Flexible tentacle structures minimize the steric hindrance between functional groups or ligand and target molecules, improving the binding capability of the target molecules. Compared to traditional packing materials, GALAK’s PS-DVB particles show more effective capture and higher recovery.
