Résine de chromatographie à perfusion Biopure AF
Biovanix Biopure AF is a rigid, hydrophilic PS-DVB perfusion chromatography resin with 3000–6000Å throughpores and flexible tentacle ligands — designed for high-flow capture of capsular viruses at 1000 cm/h with 100 mg/ml dynamic binding capacity.
√ 3000–6000Å Throughpores
√ 100 mg/ml DBC at High Flow
√ pH 1–14 Stability
√ 2–3× Throughput vs. Fast-flow Gels
√ Tentacle Ligand Technolog
Biopure AF Resin at a Glance
Key specifications for process-scale viral purification chromatography.
PS/DVB
Substrat
70 µm
Taille des particules
3000–6000Å
Taille des pores
100 mg/ml
Capacité de liaison dynamique
1000 cm/h
Débit maximum
1–14
pH Stability Range
20–40 cm
Hauteur du lit de la colonne
4–30°C
Température de fonctionnement
0.5–1M NaOH
Condition CIP
2–8°C
Storage Temperature
High-Performance Perfusion Chromatography Media
Combining innovative bonding and surface treatment techniques, Biovanix developed a series of high-performance Biopure perfusion chromatography resins. Biopure is hydrophilic porous polymer microspheres developed based on PSDVB microspheres with extremely low non-specific adsorption. Biopure media are top-performing chromatographic media for process-scale bioseparations. The rigid, robust particles enable high-resolution separations with 2–3× the throughput of conventional fast-flow gels. They are easier to handle and pack and offer outstanding cleanability.
Les milieux de chromatographie par perfusion de Biopure offrent de meilleurs choix pour vos processus de purification dans les domaines des sciences biologiques, du génie biologique et de l'industrie pharmaceutique.
Biopure AF Media Advantages
Engineered for efficiency, safety, and scalability in biopharmaceutical virus purification.
High Selectivity
Similar affinity to live and inactivated capsular viruses, enabling consistent capture across different virus states.
Closed, Safe, and Sterile
Closed-column operation ensures safety and sterility throughout the entire purification process.
Streamlined Process
Enrichment and purification are carried out simultaneously, increasing production while reducing processing steps, time, and cost.
Rigid Particles, High Flow Rate
Rigid particles can be operated under a high flow rate, significantly improving purification efficiency.
Excellent Chemical Stability
Good chemical stability delivers longer service life, reducing resin replacement frequency and operational cost.
Need help selecting the right resin for your virus purification process?
Core Technology Features
Innovative particle engineering that sets Biopure AF apart in perfusion chromatography.
Polyvalence
Multiple Choices
Supports interactifs d'échange d'ions, d'affinité et hydrophobes. Choix multiples pour différentes applications.
Particle Design
Microsphère rigide avec des pores de grande taille
The core of VirCap® particles is the novel porous structure with large “throughpores”. These large throughpores allow part of mobile phase flow through, quickly carrying biomolecules to smaller diffusive pores. The large “throughpores” reduce diffusion rate of biomolecules and enhance interaction between biomolecules and functional groups on the surface. Consequently, diffusion barriers are lowered, and flow rate can be dramatically increased — without compromising capacity or resolution.
√ 3000–6000Å pore size, enable the diffusion and mass transfer for large biomolecules.
√ 45–90 micron particles, satisfy your purification processing requests.
Ligand Architecture
Tentacules flexibles
Flexible tentacle structure minimizes the steric hindrance between functional groups and target molecules. It also improves the binding capability of the target material. Compared to traditional media, VirCap® media have more effective capture and higher total recovery.
Performance
Débit élevé, faible contre-pression
Biopure media offer an excellent balance of resolution and operating backpressure. Under the condition of mobile phase change, VirCap® media exhibits almost no shrinkage or expansion. Throughpores and flexible tentacles ensures the rapid diffusion of solute, reduces the barrier of mass transfer, and realizes the high dynamic load under the operation of high flow rate.
Durabilité
Durability Robust Chemical Stability
Les milieux Biopure sont des particules polymères rigides recouvertes d'un polymère hydrophile exclusif sur lequel les différents groupes fonctionnels (échange d'ions, affinité, etc.) sont attachés de manière covalente. Il en résulte un produit très robuste et chimiquement stable, parfaitement adapté aux applications biopharmaceutiques à grande échelle.
Maintenance
Condition de nettoyage en place (CIP)
Biopure AF supports robust cleaning-in-place protocols to ensure column hygiene and consistent performance across multiple cycles.
√ 0.5–1M NaOH
√ Organic solvent
√ High salt solvent
Biopure AF Resin Parameters
Complete technical specifications for process development and scale-up.
Biopure AF Perfusion Chromatography Resin — Technical Data Sheet
| Substrat | Microsphères hydrophiles PS/DVB |
| Taille des particules | 70um |
| Capacité de liaison dynamique | 100 mg de lysozyme/ml |
| Débit | 1000cm/h (20℃, viscosité de la solution tampon identique à celle de l'eau，pression < 3 bar / 43,5psi, hauteur du lit de la colonne 20cm). |
| Hauteur du lit de la colonne | 20-40cm |
| Stabilité du pH | 1-14 |
| Température de fonctionnement | 4-30℃ |
| Condition CIP | 0,5-1M NaOH |
| Stockage | 2-8℃ 20% EtOH |
Virus & Antigen Applications
Biopure AF resin has been successfully applied to purify a broad range of capsular viruses and viral/microbial antigens.
Validated Virus and Antigen Applications for Biopure AF Perfusion Chromatography Resin
| Virus | Antigènes viraux/microbiens | |
| Rage | Calicivirus félin | Sous-unités des glycoprotéines gA et gB de l'herpès simplex |
| Grippe | Virus respiratoire syncytial | Antigène de surface de l'hépatite B |
| Encéphalite japonaise | Herpès simplex humain | Hémagglutinine filamenteuse de B. pertussis |
| Leucémie féline | Rougeole humaine | Facteur de promotion de la leucocytose Hémagglutinine |
| Herpès félin | Parainfluenza humaine | |
Why Perfusion Chromatography for Virus Purification?
La purification des virus est la base du développement de vaccins de haute qualité et fournit également une base importante pour l'étude de la structure morphologique fine du virus, l'isolement et la purification de la protéine antigène du virus, ainsi que des études détaillées de la composition chimique et du matériel génétique du virus.
Le processus de purification ultrarapide par gradient de densité de saccharose utilisé dans les procédés traditionnels de purification des vaccins antiviraux nécessite de grands appareils centrifuges très coûteux. Ces appareils ont une faible charge d'échantillon, des temps de traitement longs et une mauvaise élimination de l'ADN de la cellule hôte. La chromatographie par filtration sur gel, largement utilisée dans le processus de purification des vaccins, est douce, reproductible et facile à industrialiser. Cependant, son efficacité est faible et l'effet de l'élimination des hétéroprotéines et de l'ADN n'est pas évident.
VirCap® AF media is an affinity chromatography media designed to capture and moderate capsular virus purification stages. Specific adsorption of VirCap® AF media and target occurs by simulating the affinity between ligands and virus particles with capsular membranes. With unique high loading capacity, high flow rate and low back pressure, VirCap® AF media reduces the process cycle time and increases the yield, fully meeting the requirements of large-scale biopharmaceutical and vaccine production processes.
Particle Microstructure & Ligand Bonding Technology
Particle SEM Picture — Biopure AF porous microsphere morphology
Technologie de liaison des ligands
Functional groups or ligand on traditional packing materials are distributed along their surface. This limits their ability to effectively interact with bio-molecules. GALAK developed technologies to build “tentacle structure” on its ion-exchange and affinity packing materials. Flexible tentacle structures minimize the steric hindrance between functional groups or ligand and target molecules, improving the binding capability of the target molecules. Compared to traditional packing materials, GALAK’s PS-DVB particles show more effective capture and higher recovery.
Application Scenarios
From vaccine development to structural virology — Biopure AF supports every stage of your purification workflow.
Vaccine Purification
High-quality vaccine development requires efficient, scalable virus capture. Biopure AF delivers one-step enrichment and purification for live and inactivated virus vaccines.
Virus Morphological Studies
Provides purified virus particles suitable for fine morphological structure analysis using electron microscopy and other advanced imaging techniques.
Antigen Protein Isolation
Isolation and purification of viral antigen proteins for downstream characterization, formulation, and quality control in biopharmaceutical manufacturing.
Genetic Material Research
Obtain highly pure virus preparations for detailed studies of viral chemical composition and genetic material, supporting genomic and proteomic analysis.
Ready to optimize your virus purification workflow?
Related Products & Complementary Solutions
Build a complete purification workflow with Biovanix resins, columns, and systems.
Chromatography Resins & Media
Resin Family
Résine Core-Shell InertShell
PMMA core-shell technology designed for inactivated virus flow-through purification. Complements Biopure AF in a capture-and-polish workflow.
Resin Family
Supermacroporous Polymer Microspheres
Ultra-large pore polymer microspheres delivering 10× binding capacity for challenging separations of very large biomolecules and viruses.
Resin Family
Supports de chromatographie PSDVB
Polystyrene-divinylbenzene-based chromatography media with customizable pore sizes from 100 nm to 400 nm for diverse separation needs.
Resin Family
Supports de chromatographie en PMMA
Polymethyl methacrylate-based media offering excellent biocompatibility and tailored surface chemistry for specific purification applications.
Équipement corollaire
Optimized chromatography columns designed to pair with Biopure AF resin.
Temperature-controlled chromatography with double-layer glass design for precise process conditions during virus purification.
Reliable low-pressure glass column for laboratory and pilot-scale virus purification with Biopure AF perfusion resin.
Frequently Asked Questions
Everything you need to know about Biopure AF perfusion chromatography resin.
What is perfusion chromatography and how does it differ from gel filtration?
Perfusion chromatography uses particles with large throughpores (3000–6000Å) that allow part of the mobile phase to flow directly through the microsphere, creating convective mass transfer. Traditional gel filtration relies entirely on diffusion to transport biomolecules into pores. This convective mechanism enables perfusion resins to maintain high resolution at 2–3× the throughput of conventional fast-flow gels.
How does the throughpore / porous structure enable high flow rate?
The large throughpores (3000–6000Å) allow part of the mobile phase to flow through the particle, quickly carrying biomolecules to smaller diffusive pores where binding occurs. This reduces the diffusion path and mass transfer resistance. Combined with flexible tentacle structures, the resin achieves linear flow rates up to 1000 cm/h at low backpressure without sacrificing binding capacity.
What are the advantages of the tentacle structure?
The flexible tentacle structure extends functional groups or ligands away from the particle surface on polymer chains, minimizing steric hindrance between the ligand and target molecules. This improves ligand accessibility and binding capacity, resulting in more effective capture and higher total recovery compared to traditional surface-bonded media.
What particle size and pore size ranges are available?
Biopure AF resin features 70 µm particle size with 3000–6000Å throughpores. The broader Biovanix PSDVB and PMMA platform also offers 45–90 µm particle sizes and pore sizes from 100 nm to 400 nm for customized separation needs.
What is the dynamic binding capacity?
Biopure AF has a dynamic binding capacity (DBC) of 100 mg lysozyme per ml of resin, maintained even at high flow rates thanks to the efficient convective mass transfer through the throughpore structure.
What flow rate and backpressure can I expect?
Biopure AF supports linear flow rates up to 1000 cm/h at 20°C with buffer viscosity equivalent to water, at less than 3 bar (43.5 psi) backpressure with a 20 cm column bed height. The rigid PS/DVB skeleton ensures minimal shrinkage or expansion during mobile phase changes.
What is the pH operating range and chemical stability?
Biopure AF is stable across the full pH range of 1–14. The rigid polymeric PS/DVB particle backbone coated with a proprietary hydrophilic polymer provides excellent chemical stability, tolerating 0.5–1M NaOH, organic solvents, and high-salt solvents for cleaning and sanitization.
Can Biopure AF be used for both live and inactivated virus purification?
Yes. Biopure AF features high selectivity with similar affinity to both live and inactivated capsular viruses. The closed-column operation ensures safety and sterility throughout the purification process.
What are the CIP and storage conditions?
Clean-in-Place (CIP) conditions include 0.5–1M NaOH, organic solvents, and high-salt solvents. Storage conditions are 2–8°C in 20% ethanol (EtOH).
Which viruses and antigens has this resin been applied to?
Biopure AF has been applied to purify Rabies, Influenza, Japanese Encephalitis, Feline Leukemia, Feline Herpes, Feline Calicivirus, Respiratory Syncytial Virus, Human Herpes Simplex, and Human Measles viruses, as well as Human Parainfluenza. Viral and microbial antigens include Herpes Simplex gA and gB Glycoprotein Subunits, Hepatitis B Surface Antigen, Filamentous Hemagglutinin from B. pertussis, and Leucocytosis Promoting Factor Hemagglutinin. A validated one-step purification protocol for live attenuated rabies virus vaccine is available.
How does Biopure AF relate to InertShell product lines?
Biopure AF is part of the VirCap series as a perfusion affinity resin specifically designed for capsular virus capture. InertShell is a separate PMMA core-shell technology product optimized for inactivated virus flow-through purification. The two product lines can be used together in an integrated capture-and-polish workflow.
What chromatography columns and equipment pair well with Biopure AF resin?
Biopure AF is compatible with Biovanix Jacketed Double-layer Glass Columns, Low-pressure Chromatography Columns, Système de remplissage de colonne HPLC Isoplex, stainless steel column tubes, and protective pre-columns. Contact Biovanix for personalized equipment recommendations based on your scale and process requirements.
Optimize Your Virus Purification Process
Contact Biovanix to discuss your specific application, request sample quantities, or get a detailed quote for Biopure AF perfusion chromatography resin.
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