Oligo dT(25) Affinity Chromatography Resin
Biovanix Oligo dT(25) Affinity Resin is a cutting-edge solution designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This advanced resin selectively captures mRNA via its polyadenylated (polyA) tail, effectively separating it from other components of the transcription reaction, such as enzymes and plasmid DNA.
Perfectly suited for large-scale downstream purification, Biovanix Oligo dT(25) Affinity Resin is a key enabler for vaccine and gene therapy applications, offering unmatched selectivity and capacity to meet the demands of modern biopharmaceutical manufacturing.
Features
1. Selective mRNA Capture
- PolyA Tail Specificity: Targets mRNA with high selectivity, ensuring effective removal of plasmid DNA and other IVT components.
- Simple Salt and Water Elution: Streamlined purification process with minimal complexity.
2. Scalable Solution
- Excellent Scalability: Designed for seamless transition from lab-scale to large-scale production.
- High Capacity: Meets the demands of high-yield mRNA manufacturing.
3. Non-Animal Derived
- Synthetic Manufacturing Process: Biovanix oligo ligands are free of animal components, ensuring compliance with regulatory requirements and ethical standards.
4. Simplified Workflow
- Reduced Complexity: Eliminates the need for multiple purification steps, maximizing efficiency and reducing downstream processing complexity.
- Consistent Performance: Reliable results across different scales and production batches.
Specification
| Characteristic | Description |
| Support Matrix | Cross-linked poly(styrene-divinylbenzene) |
| Average Particle Size | 50 um |
| Average Pore Size | 200 nm |
| Surface Functionality | poly(dT) 25mer with proprietary linker |
| Ligand Density | 0.3 umol/ml |
| Mechanical Resistance | 70 bar (1,000 psi; 7 MPa) |
| Thermal Stability | allows sample denaturing at 65°C if needed |
| pH Range | 2-13 |
| Ionic Strength Range | 0 to 5 M, all common salts |
| Chemical Resistance | Common agents for mRNA purification, include 0.5 M NaOH, 2 M MgCl2, 20 mM EDTA. Water, 0 to 100% alcohol, acetonitrile, 2 M acetic acid, 1 M HCl, and other common organic solvents |
| Storage | 18-20% ethanol |
Chemical & Thermal Resistance
| pH Range | 2–13 |
| Ionic Strength Range | 0 to 5 M, all common salts |
| Buffer Additives | Common agents for mRNA purification, include 0.5 M NaOH, 2 MMgCl2, 20 mM EDTA.
Do not expose to strong oxidizers (such as hypochlorite), oxidizing acids (such as nitric), strong reducing agents (such as sulfite), acetone, THF, or benzyl alcohol. |
| Solvents | Water, 0 to 100% alcohol, acetonitrile, 2 M acetic acid, 1 M HCl, and other common organic solvents.
Do not expose to strong oxidizers (such as hypochlorite), oxidizing acids (such as nitric), strong reducing agents (such as sulfite), acetone, benzyl alcohol, or THF. |
| Flow rate | Adjust flow rate depending on performance. Do not exceed upper-pressure limitations. |
Benefits at a Glance
High Efficiency
Rapid and efficient mRNA purification.
Scalable Process
Easily adaptable to large-scale manufacturing.
Regulatory Compliance
Non-animal derived and synthetic manufacturing process.
Cost-Effective
Simplified workflow reduces operational complexity and costs.
Applications
Vaccine Production
Efficient purification of mRNA vaccines.
Gene Therapy
Scalable production of mRNA for therapeutic applications.
IVT Manufacturing
Streamlined isolation of mRNA from transcription reaction mixtures.
Plasmid DNA Removal
Effective elimination of plasmid DNA and other IVT components.
Why Choose Biovanix Oligo dT(25) Affinity Resin?
Efficient mRNA Purification
Achieve high-purity mRNA with minimal impurities.
Cost-Effective
Reduced operational complexity and increased productivity lower overall production costs.
Regulatory Compliance
Non-animal derived and synthetic manufacturing ensure compliance with global standards.
Versatile Application
Ideal for a wide range of mRNA constructs used in vaccines, gene therapies, and other biopharmaceuticals.
Customer Testimonial
“Biovanix InertShell Chromatography Resin has transformed our virus purification process. The dual functionality and high selectivity have significantly improved our yields and purity levels, making it an essential tool in our testings.”
– Alex, Developer, AA Biopharma
