Engineered for reproducibility, high pressure resistance, superior peak resolution
Precision Prepacked HPLC Columns for Unmatched Separation Performance
High-Performance Liquid Chromatography (HPLC) columns are essential tools in modern analytical chemistry, widely used in pharmaceutical development, biomedical research, food safety testing, and environmental monitoring. HPLC columns achieve the efficient separation of complex mixtures by exploiting the differences in the distribution of solutes between the stationary phase and the mobile phase. Below is a detailed introduction to HPLC columns:
Advantages
- Stationary Phase Types: Common stationary phases include silica, polymers, and chemically modified materials such as C18, C8, NH₂, etc. Different stationary phases are suitable for the separation of compounds with varying polarities.
- Particle Size and Pore Size: Particle sizes typically range from 1.8 µm to 10 µm, while pore sizes vary depending on the application, such as 100 Å, 200 Å, and 300 Å. Smaller particles can enhance separation efficiency but may require higher operating pressures.
- Column Length and Inner Diameter: The selection of column length and inner diameter depends on the separation requirements. Longer columns generally provide higher separation efficiency.
- pH Stability: Some HPLC columns can operate stably over a broad pH range (e.g., pH 2-12).
Reversed-phase Liquid Chromatography
Normal-phase Liquid Chromatography
Application
Pharmaceutical Development
Biomedical Research
Food Safety Testing
Environmental Monitoring
HPLC Column Selection
Depending on the scale and/or efficiency of the separation required, the table below can help you to choose a column by the most appropriate inner diameter (I.D.) and column length for your needs.
Which column length is best for my needs?
- If you want to maximize the speed of your application → 20 mm to 75 mm
- If you want a balance of resolution and speed → 100 mm
- If you want the best resolution possible → 150 mm
- Also available in both analytical and semi-prep dimensions
USP (United States Pharmacopeia) columns are high-performance liquid chromatography (HPLC) columns designed to meet the stringent standards set by the USP, ensuring consistency, reliability, and compliance in analytical procedures. These columns are widely used in pharmaceutical, environmental, and food safety applications to provide accurate and reproducible results.
Why Choose Biovanix?
High-Pressure Stability
- Withstand up to 10,000 psi for UHPLC applications.
- Materials: 316L stainless steel or biocompatible PEEK.
Exceptional Peak Resolution
- Mirror-polished inner surface (Ra ≤0.2 μm) minimizes band broadening.
Customizable Options
- Column dimensions: 2.1–50 mm ID | 20–500 mm length.
- Packing materials: C18, C8, HILIC, ion exchange, affinity resins.
Wide pH Range
- Compatible with aggressive mobile phases.
Low Dead Volume Design
- Ensures minimal sample loss and high reproducibility.
