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탁월한 분리 성능을 위한 정밀 프리패킹 HPLC 컬럼

High-Performance Liquid Chromatography (HPLC) columns are essential tools in modern analytical chemistry, widely used in pharmaceutical development, biomedical research, food safety testing, and environmental monitoring. HPLC columns achieve the efficient separation of complex mixtures by exploiting the differences in the distribution of solutes between the stationary phase and the mobile phase.

10,000

Max Pressure (psi)

2.1–50

Column ID Range (mm)

pH 2–12

Stable Operating Range

20+

Column Types Available

Technical Advantages

Engineered for Reproducibility, High Pressure Resistance & Superior Peak Resolution

Every Biovanix HPLC column is designed to deliver consistent, high-quality separations across a wide range of analytical conditions.

고정 위상 유형

Common stationary phases include silica, polymers, and chemically modified materials such as C18, C8, NH₂, etc. Different stationary phases are suitable for the separation of compounds with varying polarities.

입자 크기 및 기공 크기

Particle sizes typically range from 1.8 µm to 10 µm, while pore sizes vary depending on the application, such as 100 Å, 200 Å, and 300 Å. Smaller particles can enhance separation efficiency but may require higher operating pressures.

기둥 길이 및 내경

The selection of column length and inner diameter depends on the separation requirements. Longer columns generally provide higher separation efficiency.

pH 안정성

Some HPLC columns can operate stably over a broad pH range (e.g., pH 2-12), enabling flexible method development with aggressive mobile phases.

제품 카탈로그

Comprehensive HPLC Column Portfolio

Explore our full range of prepacked chromatography columns organized by application category. Click any product to learn more.

C18 컬럼 및 벌크

C8 컬럼 및 벌크

C4 컬럼 및 벌크

페닐 컬럼 및 벌크

SiO2 컬럼 및 벌크

NH2/아미노 컬럼 및 벌크

CN/Cyano 칼럼 및 벌크

디올 칼럼 및 벌크

아미드 열

HILIC 칼럼

이온 교환 열

설탕 칼럼

SEC 칼럼

HIC 칼럼

DNA 칼럼

키랄 칼럼

단백질 A 칼럼

HbA1c 분석 열

HPLC 컬럼 튜브

PEEK 컬럼 튜빙

가드 열

인라인 필터

애플리케이션

Trusted Across Industries

From drug development to environmental protection, Biovanix HPLC columns deliver reliable performance where it matters most.

제약 개발

의약품의 안전성과 효능을 보장하기 위해 의약품 성분의 분리, 정제 및 품질 관리에 사용됩니다.

생물의학 연구

생명과학 연구를 지원하기 위해 생체 분자(예: 단백질 및 펩타이드)와 대사산물을 분석합니다.

식품 안전 테스트

식품에 포함된 첨가물 및 잔류 농약과 같은 유해 물질을 감지합니다.

환경 모니터링

물과 토양의 유기 오염 물질과 잔류 농약을 모니터링합니다.

Selection Guide

How to Choose the Right HPLC Column

Selecting the optimal column dimension and stationary phase is critical for achieving the best separation results.

필요한 분리 규모 및/또는 효율성에 따라 아래 표를 참고하여 필요에 가장 적합한 내경(I.D.)과 컬럼 길이로 컬럼을 선택할 수 있습니다.

내 요구 사항에 가장 적합한 열 길이는 무엇인가요?

애플리케이션의 속도를 최대화하려면 → 20mm에서 75mm로 확대합니다.
해상도와 속도의 균형을 원하는 경우 → 100mm
최상의 해상도를 원하는 경우 → 150mm
분석 및 반준비 차원 모두에서 사용 가능

USP(미국 약전) 컬럼은 USP에서 정한 엄격한 표준을 충족하도록 설계된 고성능 액체 크로마토그래피(HPLC) 컬럼으로, 분석 절차의 일관성, 신뢰성 및 규정 준수를 보장합니다. 이러한 컬럼은 정확하고 재현 가능한 결과를 제공하기 위해 제약, 환경 및 식품 안전 분야에서 널리 사용됩니다.

Why Biovanix

Why Choose Biovanix HPLC Columns?

Every column is engineered and manufactured to the highest standards, backed by our commitment to quality and technical support.

01

고압 안정성

Withstands up to 10,000 psi for UHPLC applications

Materials: 316L stainless steel or biocompatible PEEK

02

탁월한 피크 해상도

Mirror-polished inner surface (Ra ≤0.2 μm) minimizes band broadening, delivering sharp, well-defined peaks for precise quantification.

03

사용자 지정 가능한 옵션

열 치수: 2.1-50mm ID | 20-500mm 길이

Packing materials: C18, C8, HILIC, ion exchange, affinity resins

04

넓은 pH 범위

Compatible with aggressive mobile phases across a broad pH range, enabling flexible method development for diverse analytes.

05

낮은 데드 볼륨 설계

Ensures minimal sample loss and high reproducibility, critical for trace-level analysis and quantitative accuracy.

Need Custom Specifications?

Our engineering team can tailor column dimensions, stationary phases, and end fittings to your exact requirements.

FAQ

Frequently Asked Questions

Everything you need to know about Biovanix prepacked HPLC columns.

What are prepacked HPLC columns and how do they work?

Prepacked HPLC columns are factory-filled, ready-to-use chromatographic columns where the stationary phase (typically chemically modified silica particles) is uniformly packed under high pressure into stainless steel or PEEK tubing.

They work by exploiting differential interactions between analytes, the stationary phase, and the mobile phase. Sample components partition based on hydrophobicity, polarity, charge, or size as the mobile phase is pumped through the column at high pressure. Compounds with stronger affinity for the stationary phase are retained longer, enabling efficient separation, identification, and quantification.

What types of HPLC columns does Biovanix offer?

Biovanix offers six major categories:

Reversed-Phase Columns — C18, C18H, C18L, C18AQ, C18BS, C8, C4-300, Phenyl
Normal-Phase Columns — SiO₂, NH₂, CN, Diol, Amide
Small-Molecule Columns — HILIC, Ion Exchange (SCX/SAX), Sugar Analysis
Biological Columns — SEC (SEC-120/150/300/500/1000), HIC, DNA
Specialty Columns — Chiral (8 types), Protein A, HbA1c
소모품 — SS/PEEK column tubing, guard columns, inline filters, frits, fittings

What is the maximum operating pressure for Biovanix HPLC columns?

입자 크기	Max. 압력
10 μm	4,000 psi
5 μm	5,000 psi
3 μm	6,000 psi
SEC (3 μm)	2,500 psi
SEC (5 μm)	1,500 psi
UHPLC applications	Up to 10,000 psi

Temperature limits: 60 °C for reversed-phase columns; 40 °C for SEC columns.

Can I customize HPLC column dimensions and packing materials?

Yes. Biovanix supports full customization:

Inner diameter: 2.1–50 mm (10 standard sizes); non-standard sizes available upon request.
Column length: 20–500 mm (8 standard lengths); custom lengths accepted.
포장 재료: C18, C8, Phenyl, NH₂, CN, Diol, Bare Silica and more (particle sizes 3–50 μm; pore sizes 100/120/300 Å). Special bonded phases are available upon request.
하드웨어: 316L stainless steel or PEEK tubing; multiple frit types; custom end fittings and seals.

Are Biovanix HPLC columns suitable for pharmaceutical quality control?

Yes. Key qualifications include:

Columns carry recognized USP codes (L1, L3, L7, L8, L9, L10, L11, L14), enabling direct compliance with pharmacopeial methods.
Ultra-pure silica with metal ions < 20 ppm ensures minimal peak tailing for basic compounds.
Proprietary bonding chemistry with deep endcapping delivers low silanol activity and excellent batch-to-batch reproducibility.
pH stability from 2–8 (up to 1–12 for C18BS) covers diverse mobile phase conditions.
Manufacturing follows ISO 9001 quality standards.
Applications explicitly include drug substance characterization, impurity profiling, dissolution testing, and content uniformity analysis.

How do I select the right HPLC column for my application?

Select based on four key parameters:

① Separation mode — Match the analyte properties to the column chemistry:

Hydrophobic compounds → C18 / C8 (reversed-phase)
Aromatic compounds → Phenyl
Polar compounds → HILIC / Amide
Sugars → Sugar-10H / NH₂
Proteins/antibodies → SEC-300 / C4-300
Enantiomers → Chiral columns

② Column dimensions — Choose by scale:

Analytical QC: 2.1–4.6 mm ID × 100–250 mm length
Semi-preparative: 7.8–10 mm ID
Preparative: 20–50 mm ID

③ Particle size — Balance resolution and pressure:

3 μm: highest resolution (pharmacopeial methods)
5 μm: optimal balance (most common)
10 μm: low pressure, scalable to preparative

④ Pore size — Match to molecular weight:

100 Å: small molecules (< 3 kDa)
300 Å: peptides and proteins
SEC series: size-based separation of biomolecules

Ready to Optimize Your Chromatography?

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