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Columnas HPLC preenvasadas de precisión para un rendimiento de separación inigualable

High-Performance Liquid Chromatography (HPLC) columns are essential tools in modern analytical chemistry, widely used in pharmaceutical development, biomedical research, food safety testing, and environmental monitoring. HPLC columns achieve the efficient separation of complex mixtures by exploiting the differences in the distribution of solutes between the stationary phase and the mobile phase.

10,000

Max Pressure (psi)

2.1–50

Column ID Range (mm)

pH 2–12

Stable Operating Range

20+

Column Types Available

Technical Advantages

Engineered for Reproducibility, High Pressure Resistance & Superior Peak Resolution

Every Biovanix HPLC column is designed to deliver consistent, high-quality separations across a wide range of analytical conditions.

Tipos de fase estacionaria

Common stationary phases include silica, polymers, and chemically modified materials such as C18, C8, NH₂, etc. Different stationary phases are suitable for the separation of compounds with varying polarities.

Tamaño de las partículas y de los poros

Particle sizes typically range from 1.8 µm to 10 µm, while pore sizes vary depending on the application, such as 100 Å, 200 Å, and 300 Å. Smaller particles can enhance separation efficiency but may require higher operating pressures.

Longitud y diámetro interior de la columna

The selection of column length and inner diameter depends on the separation requirements. Longer columns generally provide higher separation efficiency.

Estabilidad del pH

Some HPLC columns can operate stably over a broad pH range (e.g., pH 2-12), enabling flexible method development with aggressive mobile phases.

Catálogo de productos

Comprehensive HPLC Column Portfolio

Explore our full range of prepacked chromatography columns organized by application category. Click any product to learn more.

Columna C18 y granel

C8 Columna y granel

C4 Columna y Bulto

Columna de fenilo y a granel

Columna de SiO2 y a granel

NH2/Amino Columna y granel

CN/Ciano Columna y granel

Columna Diol & Bulto

Columna de amidas

Columna HILIC

Columna de intercambio iónico

Columna de azúcar

Columna SEC

Columna HIC

Columna de ADN

Columna quiral

Columna de proteína A

Columna de análisis de HbA1c

Tubos para columnas de HPLC

Tubo de columna PEEK

Columna de guardia

Filtros en línea

Aplicaciones

Trusted Across Industries

From drug development to environmental protection, Biovanix HPLC columns deliver reliable performance where it matters most.

Desarrollo farmacéutico

Se utiliza para la separación, purificación y control de calidad de los componentes de los medicamentos para garantizar su seguridad y eficacia.

Investigación biomédica

Analiza biomoléculas (como proteínas y péptidos) y metabolitos para apoyar la investigación en ciencias de la vida.

Pruebas de seguridad alimentaria

Detecta sustancias nocivas como aditivos y residuos de pesticidas en los alimentos.

Vigilancia medioambiental

Controla los contaminantes orgánicos y los residuos de pesticidas en el agua y el suelo.

Selection Guide

How to Choose the Right HPLC Column

Selecting the optimal column dimension and stationary phase is critical for achieving the best separation results.

Dependiendo de la escala y/o la eficacia de la separación requerida, la tabla siguiente puede ayudarle a elegir una columna por el diámetro interior (D.I.) y la longitud de columna más adecuados a sus necesidades.

¿Qué longitud de columna se adapta mejor a mis necesidades?

Si desea maximizar la velocidad de su aplicación → 20 mm a 75 mm
Si desea un equilibrio entre resolución y velocidad → 100 mm
Si desea la mejor resolución posible → 150 mm
También disponible en dimensiones analítica y semipreparada

Las columnas USP (United States Pharmacopeia) son columnas de cromatografía líquida de alto rendimiento (HPLC) diseñadas para cumplir las estrictas normas establecidas por la USP, lo que garantiza la coherencia, la fiabilidad y el cumplimiento de los procedimientos analíticos. Estas columnas se utilizan ampliamente en aplicaciones farmacéuticas, medioambientales y de seguridad alimentaria para proporcionar resultados precisos y reproducibles.

Why Biovanix

Why Choose Biovanix HPLC Columns?

Every column is engineered and manufactured to the highest standards, backed by our commitment to quality and technical support.

01

Estabilidad a alta presión

Withstands up to 10.000 psi for UHPLC applications

Materials: 316L stainless steel or biocompatible PEEK

02

Excepcional resolución de picos

Mirror-polished inner surface (Ra ≤0.2 μm) minimizes band broadening, delivering sharp, well-defined peaks for precise quantification.

03

Opciones personalizables

Dimensiones de la columna: 2,1-50 mm ID | 20-500 mm longitud

Packing materials: C18, C8, HILIC, ion exchange, affinity resins

04

Amplio rango de pH

Compatible with aggressive mobile phases across a broad pH range, enabling flexible method development for diverse analytes.

05

Diseño de bajo volumen muerto

Ensures minimal sample loss and high reproducibility, critical for trace-level analysis and quantitative accuracy.

Need Custom Specifications?

Our engineering team can tailor column dimensions, stationary phases, and end fittings to your exact requirements.

FAQ

Frequently Asked Questions

Everything you need to know about Biovanix prepacked HPLC columns.

What are prepacked HPLC columns and how do they work?

Prepacked HPLC columns are factory-filled, ready-to-use chromatographic columns where the stationary phase (typically chemically modified silica particles) is uniformly packed under high pressure into stainless steel or PEEK tubing.

They work by exploiting differential interactions between analytes, the stationary phase, and the mobile phase. Sample components partition based on hydrophobicity, polarity, charge, or size as the mobile phase is pumped through the column at high pressure. Compounds with stronger affinity for the stationary phase are retained longer, enabling efficient separation, identification, and quantification.

What types of HPLC columns does Biovanix offer?

Biovanix offers six major categories:

Reversed-Phase Columns — C18, C18H, C18L, C18AQ, C18BS, C8, C4-300, Phenyl
Normal-Phase Columns — SiO₂, NH₂, CN, Diol, Amide
Small-Molecule Columns — HILIC, Ion Exchange (SCX/SAX), Sugar Analysis
Biological Columns — SEC (SEC-120/150/300/500/1000), HIC, DNA
Specialty Columns — Chiral (8 types), Protein A, HbA1c
Consumibles — SS/PEEK column tubing, guard columns, inline filters, frits, fittings

What is the maximum operating pressure for Biovanix HPLC columns?

Tamaño de las partículas	Máx. Presión
10 μm	4,000 psi
5 μm	5,000 psi
3 μm	6,000 psi
SEC (3 μm)	2,500 psi
SEC (5 μm)	1500 psi
UHPLC applications	Up to 10,000 psi

Temperature limits: 60 °C for reversed-phase columns; 40 °C for SEC columns.

Can I customize HPLC column dimensions and packing materials?

Yes. Biovanix supports full customization:

Inner diameter: 2.1–50 mm (10 standard sizes); non-standard sizes available upon request.
Column length: 20–500 mm (8 standard lengths); custom lengths accepted.
Material de embalaje: C18, C8, Phenyl, NH₂, CN, Diol, Bare Silica and more (particle sizes 3–50 μm; pore sizes 100/120/300 Å). Special bonded phases are available upon request.
Hardware: 316L stainless steel or PEEK tubing; multiple frit types; custom end fittings and seals.

Are Biovanix HPLC columns suitable for pharmaceutical quality control?

Yes. Key qualifications include:

Columns carry recognized USP codes (L1, L3, L7, L8, L9, L10, L11, L14), enabling direct compliance with pharmacopeial methods.
Ultra-pure silica with metal ions < 20 ppm ensures minimal peak tailing for basic compounds.
Proprietary bonding chemistry with deep endcapping delivers low silanol activity and excellent batch-to-batch reproducibility.
pH stability from 2–8 (up to 1–12 for C18BS) covers diverse mobile phase conditions.
Manufacturing follows ISO 9001 quality standards.
Applications explicitly include drug substance characterization, impurity profiling, dissolution testing, and content uniformity analysis.

How do I select the right HPLC column for my application?

Select based on four key parameters:

① Separation mode — Match the analyte properties to the column chemistry:

Hydrophobic compounds → C18 / C8 (reversed-phase)
Aromatic compounds → Phenyl
Polar compounds → HILIC / Amide
Sugars → Sugar-10H / NH₂
Proteins/antibodies → SEC-300 / C4-300
Enantiomers → Chiral columns

② Column dimensions — Choose by scale:

Analytical QC: 2.1–4.6 mm ID × 100–250 mm length
Semi-preparative: 7.8–10 mm ID
Preparative: 20–50 mm ID

③ Particle size — Balance resolution and pressure:

3 μm: highest resolution (pharmacopeial methods)
5 μm: optimal balance (most common)
10 μm: low pressure, scalable to preparative

④ Pore size — Match to molecular weight:

100 Å: small molecules (< 3 kDa)
300 Å: peptides and proteins
SEC series: size-based separation of biomolecules

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