Comparison Test Report For mAb Analysis HPLC Column
Columns: POR*S A column, Prosep Protein A column
Samples: mAb, Fusion Protein, Fusion Protein without Fc
Test Preparation
Instrument: Agilent 1260(DAD)
Column: 2.1mm × 30mm SS; AbSolut A 2.0mm × 30mm SS
Sample: Blank(MPA)
Standard Samples: mAb, Fusion Protein, Fusion Protein without Fc
Test Pictures
Conclusion
Prosep Protein A and POR*S A columns are consistent in terms of Blank peak shape, standard peak shape, sample peak shape, standard curve linearity, and quantification of known concentrations of standards and unknown concentrations of fermentation broth when tested with commonly used methods.
Prosep Protein A column can accurately determine the concentration of samples under Method I conditions.
Compared with the POR*S A column, the AbSolut A column showed a wider peak shape and lower peak height.
