Oligo dT(25) Affinity Chromatography Resin

Biovanix Oligo dT(25) Affinity Resin is a cutting-edge solution designed for the purification and isolation of mRNA from in vitro transcription (IVT) manufacturing processes. This advanced resin selectively captures mRNA via its polyadenylated (polyA) tail, effectively separating it from other components of the transcription reaction, such as enzymes and plasmid DNA.

Perfectly suited for large-scale downstream purification, Biovanix Oligo dT(25) Affinity Resin is a key enabler for vaccine and gene therapy applications, offering unmatched selectivity and capacity to meet the demands of modern biopharmaceutical manufacturing.

50 μm

Particle Size

200 nm

Pore Size

pH 2–13

Stability Range

Max Pressure

Stability Range

Product Features

Engineered for High-Performance mRNA Purification

Biovanix Oligo dT(25) Affinity Resin combines selective polyA capture with a rigid PS-DVB matrix for reliable results at any scale.

1. Selective mRNA Capture

✔ PolyA Tail Specificity: Targets mRNA with high selectivity, ensuring effective removal of plasmid DNA and other IVT components.

✔Simple Salt and Water Elution: Streamlined purification process with minimal complexity.

2. Scalable Solution

✔ Excellent Scalability: Designed for seamless transition from lab-scale to large-scale production.

✔ High Capacity: Meets the demands of high-yield mRNA manufacturing.

3. Non-Animal Derived

✔ Synthetic Manufacturing Process: Biovanix oligo ligands are free of animal components, ensuring compliance with regulatory requirements and ethical standards.

4. Simplified Workflow

✔ Reduced Complexity: Eliminates the need for multiple purification steps, maximizing efficiency and reducing downstream processing complexity.

✔ Consistent Performance: Reliable results across different scales and production batches.

How It Works

Simple 4-Step mRNA Purification Workflow

From crude IVT mixture to purified mRNA in a single affinity capture step — no complex multi-step protocols required.

①

Equilibrate

Prepare column with high-salt binding buffer (e.g., 0.5 M NaCl)

②

Load & Bind

Apply IVT mixture; polyA-tailed mRNA binds to poly(dT) ligands

③

Wash

Remove impurities (enzymes, pDNA, nucleotides) in flow-through

④

Elute

Release purified mRNA with water or low-salt buffer

Technical Specifications

Product Specifications

Comprehensive technical data for method development and process validation.

Characteristic	Description
Support Matrix	Cross-linked poly(styrene-divinylbenzene)
Average Particle Size	50 um
Average Pore Size	200 nm
Surface Functionality	poly(dT) 25mer with proprietary linker
Ligand Density	0.3 umol/ml
Mechanical Resistance	70 bar (1,000 psi; 7 MPa)
Thermal Stability	allows sample denaturing at 65°C if needed
pH Range	2-13
Ionic Strength Range	0 to 5 M, all common salts
Chemical Resistance	Common agents for mRNA purification, include 0.5 M NaOH, 2 M MgCl2, 20 mM EDTA. Water, 0 to 100% alcohol, acetonitrile, 2 M acetic acid, 1 M HCl, and other common organic solvents
Storage	18-20% ethanol

Chemical & Thermal Resistance

pH Range	2–13
Ionic Strength Range	0 to 5 M, all common salts
Buffer Additives	Common agents for mRNA purification, include 0.5 M NaOH, 2 MMgCl2, 20 mM EDTA. Do not expose to strong oxidizers (such as hypochlorite), oxidizing acids (such as nitric), strong reducing agents (such as sulfite), acetone, THF, or benzyl alcohol.
Solvents	Water, 0 to 100% alcohol, acetonitrile, 2 M acetic acid, 1 M HCl, and other common organic solvents. Do not expose to strong oxidizers (such as hypochlorite), oxidizing acids (such as nitric), strong reducing agents (such as sulfite), acetone, benzyl alcohol, or THF.
Flow rate	Adjust flow rate depending on performance. Do not exceed upper-pressure limitations.

Benefits at a Glance

Why Biovanix Oligo dT(25) Resin

Designed to maximize purity, simplify workflows, and reduce total cost of ownership.

⚡

High Efficiency

Rapid and efficient mRNA purification.

📈

Scalable Process

Easily adaptable to large-scale manufacturing.

🛡️

Regulatory Compliance

Non-animal derived and synthetic manufacturing process.

💰

Cost-Effective

Simplified workflow reduces operational complexity and costs.

Applications

Built for the mRNA Revolution

From vaccine development to gene therapy manufacturing, Biovanix Oligo dT(25) Resin supports the full spectrum of mRNA applications.

Vaccine Production

Efficient purification of mRNA vaccines.

Gene Therapy

Scalable production of mRNA for therapeutic applications.

IVT Manufacturing

Streamlined isolation of mRNA from transcription reaction mixtures.

Plasmid DNA Removal

Effective elimination of plasmid DNA and other IVT components.

Customer Testimonial

“Biovanix InertShell Chromatography Resin has transformed our virus purification process. The dual functionality and high selectivity have significantly improved our yields and purity levels, making it an essential tool in our testings.”
– Alex, Developer, AA Biopharma