Ni-TED 6HP Agarose Chromatography Media
Biovanix Agarose media is designed based on the Cytive Sepharose series. It offers high specificity and selectivity for biomolecular separations and purifications. Affinity separation can often remove contaminants that are difficult to eliminate using other chromatographic procedures. Purifications of several orders of magnitude can be achieved in a single step.
- Agarose Ni2+ chelating affinity chromatography medium;
- High dynamic adsorption capacity and fast flow rate operation;
- Mainly used for the separation and purification of histidine-labeled genetic engineering proteins.
Parameter
| Ni-TED 6HP | |
| Matrix | 6% cross-linked Agarose |
| Average Particle Size | 34μm |
| Changed Group | -NTA Ni2+ |
| Dynamic Binding
Capacity |
25 mg His/mL |
| Ligand Concentration | 90-120 μmol/mL resin |
| pH Stability, operational | 2-12 |
| pH Stability, CIP | 2-14 |
| Pressure | ≤0.3MPa |
| Temperature, operational | 4-40℃ |
| Thermostability | 120℃，30min, pH 7 |
| Flow Rate | 150 cm/h |
| Chemical Stability | Aqueous buffer, 0.01M NaOH, 0.01M HCl (1 week); 10 mM EDTA, 5 mM DTT, 5 mM TCEP, 20 mM β-mercaptoethanol, 1 M NaOH, 6 M guanidine hydrochloride (24 hours); 500 mM imidazole, 100 mM EDTA (2 hours); 30% isopropyl alcohol (20 minutes) |
| Storage | 20% EtOH, 4-30℃ |
Package
| Package | |
| Small Package | 25ml |
| 50ml | |
| 100ml | |
| 200ml | |
| 500ml | |
| 1L | |
| Production Package | 10L |
| 25L |
