InertShell Core-Shell Chromatography Resin
For Adenoviridae ADV Purification
Biovanix InertShell Chromatography Resin is a revolutionary core-shell technology-based resin designed for the purification of viruses and large biomolecules. Combining size-exclusion separation with binding chromatography, this advanced resin efficiently captures and isolates large biomolecules while allowing smaller contaminants to pass through and bind within the core. This dual functionality ensures high-purity outcomes in downstream processing.
Features
1. Core-Shell Technology
- Dual Functionality: Simultaneously performs size-exclusion separation and binding chromatography.
- Efficient Separation: Large biomolecules (e.g., inactivated viruses) are collected in the flow-through fraction (FT mode), while contaminants (<700 kDa) bind to the internal ligands.
- Optimized Design: The outer shell is hydrophilic and neutral, preventing unwanted interactions and ensuring high flow rates.
2. Advanced Material Composition
- Polymer Base: Made of polymeric methacrylate microspheres for robust durability and chemical stability.
- Active Ligand: Octylamine ligand in the core provides both anion exchange and hydrophobic interaction capabilities.
- Porous Structure: The shell has a pore size of 50-100 nm, while the core ranges from 200-500 nm, ensuring efficient separation based on molecular size.
3. High Purity and Efficiency
- Selective Capture: Effectively removes impurities such as host cell proteins, DNA fragments, endotoxins, and albumin.
- High Capacity: Designed to handle large-scale purification processes with high efficiency and scalability.
4. Compliance and Safety
- Non-Animal Derived: manufactured using synthetic processes, ensuring compliance with regulatory standards and ethical requirements.
- Stable Performance: Reliable across various production scales, ensuring consistent results.
Specification
| Biovanix Inert Shell | Competitor 700 | |
| Matrix | Polyacrylate | Highly cross‑linked agarose |
| Ligand | Octylamine | Octylamine |
| Average particle size | 50-150 μm | 50-150 μm |
| Density of ligand | 0.10-0.20 mmol/mL | 0.04-0.085 mmol/mL |
| Binding capacity¹ | 20 mg BSA/mL resin | 12 mg BSA/mL resin |
| Operational pressure | ≤1.0 MPa | ≤0.3 MPa |
| Operational flow rate | 100-600 cm/h | 100-600 cm/h |
| pH stability | 3-13 | 3-13 |
| Temperature | 4-30℃ | 4-30℃ |
| Chemical stability | All commonly used aqueous buffers, 1 M sodium hydroxide (NaOH)², 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol. | |
| Storage | 20% ethanol at 4°C to 25 ℃ | |
- Dynamic binding capacity measured at 5% breakthrough with 76 cm/h on φ10×13 mm, 1 mL columns. The buffer was 1.0 mg/mL BSA 50 mM NaCl, pH 0.
- No significant changes in ionic capacity and carbon content after storage 1 week in 1 M NaOH at 25°C.
Compared with Competitor 700, the thickness of the core in Biovanix Inert Shell (0.5-1.0 μm) is smaller than Competitor 700 (5 μm). Biovanix Inert Shell is conducive to the rapid mass transfer of impure proteins to the medium core for capture, like cell proteins of the host, DNA fragments, endotoxin, serum. Biovanix Inert Shell has a higher yield amount of miscellaneous protein. With the macroporous structure (200-500 nm) of the core in Biovanix Inert Shell can quickly remove the miscellaneous protein in CIP. Biovanix Inert Shell also has a longer service life in the purification process. In animal vaccine studies, the performance of Biovnanix Inert Shell can be repeated use more than 30 times with less change in its properties.
Applications
Virus Purification
Effective isolation of inactivated viruses for vaccines and gene therapies.
Large Biomolecule Separation
Suitable for the purification of large proteins and complexes.
Host Cell Protein Removal
Efficient elimination of host cell proteins and other impurities.
Vaccine Production
Streamlined downstream processing for mRNA vaccines and viral vector-based vaccines.
Gene Therapy
Scalable production of viral vectors for gene therapy applications.
Why Choose Biovanix InertShell Chromatography Resin?
Enhanced Purity
Efficient removal of impurities to achieve ultra-high purity in virus and biomolecule purification.
Dual Mode Operation
Combines size-exclusion and binding chromatography for versatile and precise control.
Scalable Solution
Ideal for both lab-scale and large-scale manufacturing processes.
Cost-Effective
Simplified process flows and high efficiency reduce operational costs and improve productivity.
Regulatory Compliance
Non-animal derived and synthetic manufacturing ensure adherence to global biopharmaceutical standards.
Customer Testimonial
“Biovanix Oligo dT(25) Affinity Resin has revolutionized our mRNA purification process. The resin’s high selectivity and capacity have significantly improved our yield and purity, enabling us to meet the growing demand for mRNA vaccines.”
– John, Bioprocess Engineer, A Biopharma
