Oligo dT(25) Affinity Chromatography Resin

Résine de chromatographie d'affinité Oligo dT(25)

Résine d'affinité Biovanix Oligo dT(25) est une solution de pointe conçue pour la purification et l'isolement de l'ARNm à partir de processus de fabrication de transcription in vitro (IVT). Cette résine avancée capture sélectivement l'ARNm via sa queue polyadénylée (polyA), le séparant ainsi efficacement des autres composants de la réaction de transcription, tels que les enzymes et l'ADN plasmidique.

Parfaitement adapté à la purification à grande échelle en aval, Résine d'affinité Biovanix Oligo dT(25) est un facilitateur clé pour les applications de vaccins et de thérapies géniques, offrant une sélectivité et une capacité inégalées pour répondre aux exigences de la fabrication biopharmaceutique moderne.

50 μm

Taille des particules

200 nm

Taille des pores

pH 2–13

Stability Range

Pression maximale

Stability Range

Product Features

Engineered for High-Performance mRNA Purification

Biovanix Oligo dT(25) Affinity Resin combines selective polyA capture with a rigid PS-DVB matrix for reliable results at any scale.

1. Capture sélective d'ARNm

✔ PolyA Tail Specificity: Cible l'ARNm avec une grande sélectivité, assurant l'élimination efficace de l'ADN plasmidique et d'autres composants IVT.

✔Simple Salt and Water Elution: Procédé de purification simplifié avec une complexité minimale.

2. Solution évolutive

✔ Excellente scalabilité : Conçu pour une transition transparente de l'échelle du laboratoire à la production à grande échelle.

✔ High Capacity: Répond aux exigences de la fabrication d'ARNm à haut rendement.

3. Non dérivé d'animaux

✔ Procédé de Fabrication Synthétique : Les ligands oligo Biovanix sont exempts de composants d'origine animale, garantissant ainsi la conformité aux exigences réglementaires et aux normes éthiques.

4. Workflow simplifié

✔ Complexité réduite : Élimine le besoin de plusieurs étapes de purification, maximisant ainsi l'efficacité et réduisant la complexité des processus en aval.

✔ Performances constantes Des résultats fiables sur différentes échelles et lots de production.

How It Works

Simple 4-Step mRNA Purification Workflow

From crude IVT mixture to purified mRNA in a single affinity capture step — no complex multi-step protocols required.

①

Equilibrate

Prepare column with high-salt binding buffer (e.g., 0.5 M NaCl)

②

Load & Bind

Apply IVT mixture; polyA-tailed mRNA binds to poly(dT) ligands

③

Wash

Remove impurities (enzymes, pDNA, nucleotides) in flow-through

④

Elute

Release purified mRNA with water or low-salt buffer

Technical Specifications

Product Specifications

Comprehensive technical data for method development and process validation.

Caractéristique	Description
Matrice de soutien	Poly(styrène-divinylbenzène) réticulé
Taille moyenne des particules	50 µm
Taille moyenne des pores	200 nm
Fonctionnalité de surface	poly(dT) 25-mère avec lieur propriétaire
Densité du ligand	0,3 µmol/ml
Résistance mécanique	70 bars (1 000 psi ; 7 MPa)
Stabilité thermique	permet la dénaturation d'échantillons à 65°C si nécessaire
Gamme de pH	2-13
Gamme de force ionique	0 à 5 M, tous les sels courants
Résistance chimique	Les agents couramment utilisés pour la purification de l'ARNm comprennent le NaOH 0,5 M, le MgCl₂ 2 M et l'EDTA 20 mM. L'eau, l'alcool 0 à 100%, l'acétonitrile, l'acide acétique 2 M, l'HCl 1 M et d'autres solvants organiques courants
Stockage	18-20% éthanol

Résistance chimique et thermique

Gamme de pH	2–13
Gamme de force ionique	0 à 5 M, tous les sels courants
Additifs de tampon	Les agents courants pour la purification de l'ARNm comprennent 0,5 M de NaOH, 2 mM de MgCl2, 20 mM d'EDTA. Ne pas exposer à des oxydants forts (tels que l'hypochlorite), des acides oxydants (tels que l'acide nitrique), des agents réducteurs forts (tels que le sulfite), l'acétone, le THF ou l'alcool benzylique.
Solvants	Eau, alcool 0 à 100%, acétonitrile, acide acétique 2 M, HCl 1 M et autres solvants organiques courants. Ne pas exposer aux agents d'oxydation forts (tels que l'hypochlorite), aux acides oxydants (tels que l'acide nitrique), aux agents réducteurs forts (tels que le sulfite), à l'acétone, à l'alcool benzylique ou au THF.
Débit	Ajuster le débit en fonction des performances. Ne pas dépasser les limites de pression supérieures.

Avantages en un coup d'œil

Why Biovanix Oligo dT(25) Resin

Designed to maximize purity, simplify workflows, and reduce total cost of ownership.

⚡

Haute efficacité

Purification rapide et efficace de l'ARNm.

📈

Procédé évolutif

Facilement adaptable à la fabrication à grande échelle.

🛡️

Conformité réglementaire

Procédé de fabrication non dérivé d'animaux et synthétique.

💰

Rentable

Un flux de travail simplifié réduit la complexité opérationnelle et les coûts.

Applications

Built for the mRNA Revolution

From vaccine development to gene therapy manufacturing, Biovanix Oligo dT(25) Resin supports the full spectrum of mRNA applications.

Production de vaccins

Purification efficace des vaccins à ARNm.

Thérapie génique

Production évolutive d'ARN messager pour des applications thérapeutiques.

Fabrication IVT

Purification simplifiée de l'ARN messager à partir de milieux de réactions de transcription.

Suppression de l'ADN plasmidique

Élimination efficace de l'ADN plasmidique et des autres composants de la réaction de transcription in vitro.

Why Choose Biovanix

Your Trusted Partner in mRNA Purification

① Efficient mRNA Purification

Obtenez de l'ARNm de haute pureté avec un minimum d'impuretés.

② Cost-Effective

La réduction de la complexité opérationnelle et l'augmentation de la productivité diminuent les coûts de production globaux.

③ Regulatory Compliance

La fabrication non dérivée d'animaux et synthétique garantit la conformité aux normes mondiales.

④ Versatile Application

Idéal pour un large éventail de constructions ARNm utilisées dans les vaccins, les thérapies géniques et autres produits biopharmaceutiques.

Frequently Asked Questions

Oligo dT(25) Resin FAQ

Common questions about mRNA purification with Oligo dT(25) affinity chromatography.

What is Oligo dT(25) affinity chromatography resin and how does it work?

Oligo dT(25) affinity chromatography resin is a specialized chromatography medium functionalized with poly(deoxythymidine) 25-mer ligands attached to a solid support matrix. It works through complementary A-T base pairing: the poly(dT) ligands on the resin selectively bind to the polyadenylated (polyA) tail of mRNA molecules. When an IVT (in vitro transcription) mixture is loaded onto the column under high-salt conditions, mRNA is captured while impurities such as enzymes, plasmid DNA, and unincorporated nucleotides pass through. Elution is achieved with water or low-salt buffer, releasing the purified mRNA.

What is the principle behind mRNA purification using oligo dT affinity chromatography?

The purification principle relies on specific Watson-Crick A-T base pairing between the poly(dT) 25-mer ligand immobilized on the resin and the polyA tail present on mature mRNA. Under high ionic strength conditions (typically 0.5 M NaCl), electrostatic shielding stabilizes the hybridization between poly(dT) and polyA. The mRNA binds selectively to the resin while process impurities flow through. Elution is performed under low-ionic-strength conditions (water or dilute buffer), which destabilizes the hybrid and releases purified mRNA in a single chromatographic step.

What types of mRNA can be purified with Oligo dT(25) resin?

Oligo dT(25) resin can purify any polyadenylated mRNA produced through in vitro transcription (IVT), including mRNA constructs for vaccines (such as infectious disease vaccines), gene therapy mRNAs (such as those encoding therapeutic proteins or gene editing components), and research-grade mRNAs of various lengths. The resin is compatible with mRNA constructs ranging from short transcripts to several thousand nucleotides in length, as long as they contain a polyA tail.

What is the binding capacity of Oligo dT(25) resin?

The binding capacity of Oligo dT(25) resin depends on the mRNA construct length, loading conditions, and the specific product formulation. Generally, affinity resins with poly(dT) ligands offer high dynamic binding capacity suitable for preparative-scale mRNA purification. Smaller mRNA constructs tend to achieve higher binding capacity per mL of resin due to reduced steric hindrance. For specific capacity data for your mRNA construct, contact the Biovanix technical team for application-specific recommendations.

What are the advantages of PS-DVB matrix vs. agarose for mRNA purification?

Poly(styrene-divinylbenzene) (PS-DVB) matrices offer several advantages over traditional agarose gels for mRNA purification: (1) Higher mechanical strength — PS-DVB beads are rigid, enabling higher flow rates and pressure tolerance (up to 70 bar for Biovanix resin) without bed compression; (2) Broader chemical compatibility — stable across pH 2–13 and resistant to organic solvents, enabling aggressive cleaning-in-place (CIP) with NaOH; (3) Longer resin lifetime — the rigid polymer backbone does not compress or degrade over repeated cycles; (4) Better scalability — linear pressure-flow behavior allows consistent performance from lab to production scale; (5) No risk of gel compression artifacts during scale-up.

How do I pack and use Oligo dT(25) resin in my chromatography system?

General workflow: (1) Equilibrate the column with binding buffer (high-salt buffer, e.g., 0.5 M NaCl) to prepare the resin for mRNA capture; (2) Load the IVT reaction mixture onto the column — the mRNA polyA tail binds to the poly(dT) ligands while impurities flow through; (3) Wash the column with binding buffer or a moderate-salt wash buffer to remove residual impurities; (4) Elute purified mRNA with water or low-salt buffer (e.g., 10 mM Tris-HCl); (5) Clean and regenerate the column with appropriate CIP solution (e.g., 0.1–0.5 M NaOH) for reuse. Always adjust flow rate to stay within the pressure limit (70 bar maximum for Biovanix resin). For denaturing secondary structures, samples can be heated to 65°C during processing.

What flow rate and pressure should I use with Oligo dT(25) resin?

Biovanix Oligo dT(25) resin has a maximum mechanical resistance of 70 bar (1,000 psi / 7 MPa). The flow rate should be adjusted based on your specific application and column dimensions, ensuring that backpressure does not exceed this limit. The 50 μm average particle size allows efficient mass transfer at moderate to high linear flow rates. Start at a conservative flow rate during method development and optimize based on binding efficiency and recovery. The rigid PS-DVB matrix maintains stable bed volume across flow rate changes, providing consistent performance.

Can I reuse the Oligo dT(25) resin and how do I clean it?

Yes, Oligo dT(25) resin can be reused for multiple purification cycles with proper cleaning and storage. Cleaning-in-place (CIP) is typically performed using 0.1–0.5 M NaOH, which effectively removes residual nucleic acids and contaminants. The resin’s broad pH stability (2–13) and chemical resistance support stringent cleaning protocols. After CIP, re-equilibrate the column with binding buffer before the next use. Store the resin in 18–20% ethanol at 2–8°C when not in use. Do not freeze. Resin lifetime depends on the application, cleaning protocol, and feed material quality.

What buffer conditions are recommended for mRNA binding and elution?

For mRNA binding (capture): use a high-salt buffer such as 0.5 M NaCl with an appropriate buffer component (e.g., 10 mM Tris-HCl, pH 7.5). The high ionic strength provides electrostatic shielding that stabilizes the A-T hybridization between poly(dT) ligands and the mRNA polyA tail. For washing: use the same high-salt binding buffer or a moderately reduced salt concentration. For elution: use deionized water or a low-salt buffer (e.g., 10 mM Tris-HCl without NaCl) to destabilize the hybrid and release purified mRNA. The resin also allows sample denaturing at 65°C if needed to eliminate undesired higher-order RNA structures.

How does Oligo dT(25) resin remove plasmid DNA and other IVT impurities?

The selectivity of Oligo dT(25) resin is based on the specific A-T base pairing between the poly(dT) ligands and the polyA tail of mRNA. Only polyadenylated mRNA molecules bind to the resin. Plasmid DNA (which lacks a polyA tail), RNA polymerase enzymes, unincorporated nucleotides, short abortive transcripts, and buffer components do not bind and are removed in the flow-through and wash fractions. This single-step affinity capture effectively separates full-length mRNA from the major IVT process impurities, significantly reducing downstream processing complexity.

Can Oligo dT(25) resin be used for continuous or automated mRNA purification?

Yes, Oligo dT(25) resin is compatible with standard chromatography systems and can be adapted for automated and continuous processing workflows. The rigid PS-DVB matrix provides consistent pressure-flow behavior, making it suitable for use on automated chromatography platforms. The simple salt-and-water elution workflow minimizes buffer exchange requirements. For process development and screening, smaller column formats can be used to optimize conditions before scaling to larger automated systems. The linear scalability of the PS-DVB matrix ensures that conditions optimized at small scale translate directly to production scale.

What is the shelf life and storage condition for Oligo dT(25) resin?

Biovanix Oligo dT(25) resin should be stored in 18–20% ethanol solution. The recommended storage temperature is 2–8°C (refrigerated). Do not freeze the resin, as freezing may damage the bead structure. When properly stored, the resin maintains its performance characteristics over extended periods. Before use, ensure the resin is at room temperature and thoroughly equilibrated with binding buffer. Refer to the product certificate of analysis for specific shelf-life information for your lot.

Témoignage client

Trusted by Biopharma Professionals

“La résine chromatographique Biovanix InertShell a transformé notre processus de purification des virus. Sa double fonctionnalité et sa haute sélectivité ont considérablement amélioré nos rendements et nos niveaux de pureté, en faisant un outil essentiel dans nos tests.”
– Alex, Développeur, AA Biopharma

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