Supermacroporous Polymer Microspher
In order to address key issues in the purification of viruses and viral particles, Biovanix has always been bold in its exploration of process technology and product development. Its research and production of super macroporous ion exchange chromatography media have achieved precise control over the pore size of the chromatography media. Compared to conventional chromatography media, it has better performance in terms of load capacity and processing speed. It is more conducive to maintaining the structure of viral vectors and viral-like particles.
Vorteile
- Large molecules or viral particles can easily enter the pores for binding;
- The load capacity is more than 10 times that of conventional agarose media and
twice that of conventional polymer media; - It can maintain the integrity of the structure of large molecular proteins, obtaining high yield and high activity of the target protein;
- After hydrophilic modification of the microsphere surface and the bonding of ion exchange groups, non-specific adsorption is low, and the degree of mechanization is high;
- The molecular mass transfer rate is fast, and better separation can be achieved
at higher flow rates.
Microsphere Selection
PS-DVB Particle Selection
Produkt | Poly15 SP | Poly15 Q | Poly30 SP | Poly30 Q |
Matrix | Monodisperse PS-DVB | |||
Partikelgröße | 15um | 30um | ||
Function Group | (-CH2)SO3- | -CH2N+(CH3)3 | (-CH2)SO3- | -CH2N+(CH3)3 |
Ligandenkonzentration | 0.22meq/mL | 0.24meq/mL | 0.15meq/mL | 0.18meq/mL |
Kapazität | 80mg Lys/mL | 45mg BSA/mL | 60mg Lys/mL | 30mg BSA/mL |
Durchflussmenge | 150~800cm/h | 250~1000cm/h | ||
Max. Druck | 8.0MPa | 5.0MPa | ||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers,1M acetic acid,1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea, and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | Poly 50M | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PS-DVB | |||
Function Group | 50um | |||
Porengröße | 100-150nm | |||
Ligandenkonzentration | 0.15meq/mL | 0.16meq/mL | 0.15me q/mL | 0.16me q/mL |
Kapazität | >80mgLys | >100mg BSA | >80mgLys | >90mg BSA |
Durchflussmenge | 300~1200cm/h | |||
Max Pressure | 3.0MPa | |||
pH Stability | 1-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | Poly 50G | |||
Matrix | SP | SP | SP | SP |
Partikelgröße | PS-DVB | |||
Function Group | 50um | |||
Porengröße | 150-300nm | |||
Ligandenkonzentration | 0.14me q/mL | 0.14me q/mL | 0.14me q/mL | 0.14me q/mL |
Kapazität | >70mg Lys | >70mg Lys | >70mg Lys | >70mg Lys |
Durchflussmenge | 300~1200cm/h | |||
Max Pressure | 2.0MPa | |||
pH Stability | 1-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | Poly 50V | |||
Matrix | SP | SP | SP | SP |
Partikelgröße | PS-DVB | |||
Function Group | 50um | |||
Porengröße | 300-400nm | |||
Ligandenkonzentration | 0.12me q/mL | 0.12me q/mL | 0.12me q/mL | 0.12me q/mL |
Kapazität | >70mg Lys | >70mg Lys | >70mg Lys | >70mg Lys |
Durchflussmenge | 300~1200cm/h | |||
Max Pressure | 1.0MPa | |||
pH Stability | 1-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
PMMA Particle Selection
Produkt | PM 70S | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PMMA | |||
Function Group | 70um | |||
Porengröße | 100nm | |||
Ligandenkonzentration | 0.18 meq/mL | 0.12meq/mL | 0.21meq/mL | 0.15meq/mL |
Kapazität | 115 mg Lys | 80mg BSA | 105 mg Lys | 80mg BSA |
Durchflussmenge | 150~750cm/h | |||
Max Pressure | 1.0MPa | |||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | PM 70M | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PMMA | |||
Function Group | 70um | |||
Porengröße | 100-150nm | |||
Ligandenkonzentration | 0.15meq/mL | 0.12m eq/mL | 0.22meq/ mL | 0.11meq/mL |
Kapazität | 115 mg Lys | 80m g BSA | 105mg Lys | 80mg BSA |
Durchflussmenge | 150~750cm/h | |||
Max Pressure | 0.8MPa | |||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | PM 70G | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PMMA | |||
Function Group | 70um | |||
Porengröße | 150-300nm | |||
Ligandenkonzentration | 0.11meq/mL | 0.12meq/mL | 0.10meq/mL | 0.09meq/mL |
Kapazität | > 70mg Lys | > 75mg BSA | > 70mg Lys | > 60mg BSA |
Durchflussmenge | 150~750cm/h | |||
Max Pressure | 0.5MPa | |||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | PM 70G | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PMMA | |||
Function Group | 70um | |||
Porengröße | 150-300nm | |||
Ligandenkonzentration | 0.11meq/mL | 0.12meq/mL | 0.10meq/mL | 0.09meq/ mL |
Kapazität | > 70mg Lys | > 75mg BSA | > 70m g Lys | > 60mg BSA |
Durchflussmenge | 150~750cm/h | |||
Max Pressure | 0.5MPa | |||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |
Produkt | PM 70V | |||
Matrix | SP | Q | CM | DEAE |
Partikelgröße | PMMA | |||
Function Group | 70um | |||
Porengröße | 300-400nm | |||
Ligandenkonzentration | 0.11meq/mL | 0.12meq/mL | 0.10meq/mL | 0.09meq/ mL |
Kapazität | > 70mg Lys | > 75mg BSA | > 70m g Lys | > 60mg BSA |
Durchflussmenge | 150~750cm/h | |||
Max Pressure | 0.5MPa | |||
pH Stability | 2-12 | |||
Chemische Beständigkeit | All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants. | |||
Usage Temperature | 4~30°C | |||
Lagerung | 2~30°C, 20% ethanol |