Supermacroporous Polymer Microspher

In order to address key issues in the purification of viruses and viral particles, Biovanix has always been bold in its exploration of process technology and product development. Its research and production of super macroporous ion exchange chromatography media have achieved precise control over the pore size of the chromatography media. Compared to conventional chromatography media, it has better performance in terms of load capacity and processing speed. It is more conducive to maintaining the structure of viral vectors and viral-like particles.

Vorteile

  • Large molecules or viral particles can easily enter the pores for binding;
  • The load capacity is more than 10 times that of conventional agarose media and
    twice that of conventional polymer media;
  • It can maintain the integrity of the structure of large molecular proteins, obtaining high yield and high activity of the target protein;
  • After hydrophilic modification of the microsphere surface and the bonding of ion exchange groups, non-specific adsorption is low, and the degree of mechanization is high;
  • The molecular mass transfer rate is fast, and better separation can be achieved
    at higher flow rates.

Microsphere Selection

PS-DVB Particle Selection

Produkt Poly15 SP Poly15 Q Poly30 SP Poly30 Q
Matrix Monodisperse PS-DVB
Partikelgröße 15um 30um
Function Group (-CH2)SO3- -CH2N+(CH3)3 (-CH2)SO3- -CH2N+(CH3)3
Ligandenkonzentration 0.22meq/mL 0.24meq/mL 0.15meq/mL 0.18meq/mL
Kapazität 80mg Lys/mL 45mg BSA/mL 60mg Lys/mL 30mg BSA/mL
Durchflussmenge 150~800cm/h 250~1000cm/h
Max. Druck 8.0MPa 5.0MPa
pH Stability 2-12
  Chemische Beständigkeit All commonly used buffers,1M acetic acid,1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea, and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt Poly 50M
Matrix SP Q CM DEAE
Partikelgröße PS-DVB
Function Group 50um
Porengröße 100-150nm
Ligandenkonzentration 0.15meq/mL 0.16meq/mL 0.15me q/mL 0.16me q/mL
Kapazität >80mgLys >100mg BSA >80mgLys >90mg BSA
Durchflussmenge 300~1200cm/h
Max Pressure 3.0MPa
pH Stability 1-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt Poly 50G
Matrix SP SP SP SP
Partikelgröße PS-DVB
Function Group 50um
Porengröße 150-300nm
Ligandenkonzentration 0.14me q/mL 0.14me q/mL 0.14me q/mL 0.14me q/mL
Kapazität >70mg Lys >70mg Lys >70mg Lys >70mg Lys
Durchflussmenge 300~1200cm/h
Max Pressure 2.0MPa
pH Stability 1-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt Poly 50V
Matrix SP SP SP SP
Partikelgröße PS-DVB
Function Group 50um
Porengröße 300-400nm
Ligandenkonzentration 0.12me q/mL 0.12me q/mL 0.12me q/mL 0.12me q/mL
Kapazität >70mg Lys >70mg Lys >70mg Lys >70mg Lys
Durchflussmenge 300~1200cm/h
Max Pressure 1.0MPa
pH Stability 1-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

PMMA Particle Selection

Produkt PM 70S
Matrix SP Q CM DEAE
Partikelgröße PMMA
Function Group 70um
Porengröße 100nm
Ligandenkonzentration 0.18 meq/mL 0.12meq/mL 0.21meq/mL 0.15meq/mL
Kapazität 115 mg Lys 80mg BSA 105 mg Lys 80mg BSA
Durchflussmenge 150~750cm/h
Max Pressure 1.0MPa
pH Stability 2-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt PM 70M
Matrix SP Q CM DEAE
Partikelgröße PMMA
Function Group 70um
Porengröße 100-150nm
Ligandenkonzentration 0.15meq/mL 0.12m eq/mL 0.22meq/ mL 0.11meq/mL
Kapazität 115 mg Lys 80m g BSA 105mg Lys 80mg BSA
Durchflussmenge 150~750cm/h
Max Pressure 0.8MPa
pH Stability 2-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol, 30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt PM 70G
Matrix SP Q CM DEAE
Partikelgröße PMMA
Function Group 70um
Porengröße 150-300nm
Ligandenkonzentration 0.11meq/mL 0.12meq/mL 0.10meq/mL 0.09meq/mL
Kapazität > 70mg Lys > 75mg BSA > 70mg Lys > 60mg BSA
Durchflussmenge 150~750cm/h
Max Pressure 0.5MPa
pH Stability 2-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt PM 70G
Matrix SP Q CM DEAE
Partikelgröße PMMA
Function Group 70um
Porengröße 150-300nm
Ligandenkonzentration 0.11meq/mL 0.12meq/mL 0.10meq/mL 0.09meq/ mL
Kapazität > 70mg Lys > 75mg BSA > 70m g Lys > 60mg BSA
Durchflussmenge 150~750cm/h
Max Pressure 0.5MPa
pH Stability 2-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

 

Produkt PM 70V
Matrix SP Q CM DEAE
Partikelgröße PMMA
Function Group 70um
Porengröße 300-400nm
Ligandenkonzentration 0.11meq/mL 0.12meq/mL 0.10meq/mL 0.09meq/ mL
Kapazität > 70mg Lys > 75mg BSA > 70m g Lys > 60mg BSA
Durchflussmenge 150~750cm/h
Max Pressure 0.5MPa
pH Stability 2-12
Chemische Beständigkeit All commonly used buffers, 1M acetic acid, 1M sodium oxychloride, 1M hydrochloric acid, 70% ethanol 30% isopropyl alcohol,30% acetonitrile, 1%SDS, 6M guanidine hydrochloride, 8M urea and other commonly used organic solvents; Avoid exposure to strong oxidants.
Usage Temperature 4~30°C
Lagerung 2~30°C, 20% ethanol

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