Biopure AF Perfusion-Chromatographie-Harz
Biovanix Biopure AF is a rigid, hydrophilic PS-DVB perfusion chromatography resin with 3000–6000Å throughpores and flexible tentacle ligands — designed for high-flow capture of capsular viruses at 1000 cm/h with 100 mg/ml dynamic binding capacity.
√ 3000–6000Å Throughpores
√ 100 mg/ml DBC at High Flow
√ pH 1–14 Stability
√ 2–3× Throughput vs. Fast-flow Gels
√ Tentacle Ligand Technolog
Biopure AF Resin at a Glance
Key specifications for process-scale viral purification chromatography.
PS/DVB
Substrat
70 µm
Partikelgröße
3000–6000Å
Porengröße
100 mg/ml
Dynamische Bindungskapazität
1000 cm/h
Maximale Durchflussmenge
1–14
pH Stability Range
20–40 cm
Column Bed Height
4–30°C
Betriebstemperatur
0.5–1M NaOH
CIP Condition
2–8°C
Storage Temperature
High-Performance Perfusion Chromatography Media
Combining innovative bonding and surface treatment techniques, Biovanix developed a series of high-performance Biopure perfusion chromatography resins. Biopure is hydrophilic porous polymer microspheres developed based on PSDVB microspheres with extremely low non-specific adsorption. Biopure media are top-performing chromatographic media for process-scale bioseparations. The rigid, robust particles enable high-resolution separations with 2–3× the throughput of conventional fast-flow gels. They are easier to handle and pack and offer outstanding cleanability.
Biopure perfusion chromatography media offers better choices for your purification processes in biological science, biological engineering, and pharmaceutical industry areas.
Biopure AF Media Advantages
Engineered for efficiency, safety, and scalability in biopharmaceutical virus purification.
High Selectivity
Similar affinity to live and inactivated capsular viruses, enabling consistent capture across different virus states.
Closed, Safe, and Sterile
Closed-column operation ensures safety and sterility throughout the entire purification process.
Streamlined Process
Enrichment and purification are carried out simultaneously, increasing production while reducing processing steps, time, and cost.
Rigid Particles, High Flow Rate
Rigid particles can be operated under a high flow rate, significantly improving purification efficiency.
Excellent Chemical Stability
Good chemical stability delivers longer service life, reducing resin replacement frequency and operational cost.
Need help selecting the right resin for your virus purification process?
Core Technology Features
Innovative particle engineering that sets Biopure AF apart in perfusion chromatography.
Vielseitigkeit
Multiple Choices
Ion-exchange, affinity and hydrophobic interactive media. Multiple choices for different application.
Particle Design
Rigid Microsphere With Large Pore Size
The core of VirCap® particles is the novel porous structure with large “throughpores”. These large throughpores allow part of mobile phase flow through, quickly carrying biomolecules to smaller diffusive pores. The large “throughpores” reduce diffusion rate of biomolecules and enhance interaction between biomolecules and functional groups on the surface. Consequently, diffusion barriers are lowered, and flow rate can be dramatically increased — without compromising capacity or resolution.
√ 3000–6000Å pore size, enable the diffusion and mass transfer for large biomolecules.
√ 45–90 micron particles, satisfy your purification processing requests.
Ligand Architecture
Flexible Tentacles
Flexible tentacle structure minimizes the steric hindrance between functional groups and target molecules. It also improves the binding capability of the target material. Compared to traditional media, VirCap® media have more effective capture and higher total recovery.
Leistung
High Flow Rate, Low Backpressure
Biopure media offer an excellent balance of resolution and operating backpressure. Under the condition of mobile phase change, VirCap® media exhibits almost no shrinkage or expansion. Throughpores and flexible tentacles ensures the rapid diffusion of solute, reduces the barrier of mass transfer, and realizes the high dynamic load under the operation of high flow rate.
Durability
Durability Robust Chemical Stability
Biopure media are rigid polymeric particles coated with a proprietary hydrophilic polymer onto which the various functional groups (ion exchange, affinity, etc.) are covalently attached. The result is a highly robust, chemically stable product that is ideally suited for large-scale biopharmaceutical applications.
Maintenance
Clean-in-Place Condition (CIP)
Biopure AF supports robust cleaning-in-place protocols to ensure column hygiene and consistent performance across multiple cycles.
√ 0.5–1M NaOH
√ Organic solvent
√ High salt solvent
Biopure AF Resin Parameters
Complete technical specifications for process development and scale-up.
Biopure AF Perfusion Chromatography Resin — Technical Data Sheet
| Substrat | Hydrophilic PS/DVB Microspheres |
| Partikelgröße | 70 µm |
| Dynamic Binding Capability | 100mg lysozyme/ml |
| Durchflussmenge | 1000cm/h (20℃, buffer solution viscosity same as water，pressure < 3 bar / 43.5psi, column bed height 20cm) |
| Column Bed Height | 20-40cm |
| PHP-Stabilität | 1-14 |
| Betriebstemperatur | 4-30℃ |
| CIP Condition | 0.5-1M NaOH |
| Lagerung | 2-8℃ 20% EtOH |
Virus & Antigen Applications
Biopure AF resin has been successfully applied to purify a broad range of capsular viruses and viral/microbial antigens.
Validated Virus and Antigen Applications for Biopure AF Perfusion Chromatography Resin
| Viruses | Viral/Microbial Antigens | |
| Rabies | Feline Calicivirus | Herpes Simplex gA and gB Glycoprotein Subunits |
| Influenza | Respiratory Syncytial Virus | Hepatitis B Surface Antigen |
| Japanese Enchephalitis | Human Herpes Simplex | Filamentous Hemagglutinin from B. pertussis |
| Feline Leukemia | Human Measles | Leucocytosis Promoting Factor Hemagglutinin |
| Feline Herpes | Human Parainfluenza | |
Why Perfusion Chromatography for Virus Purification?
Virus purification is the basis of developing high-quality vaccines and also provides an important basis for the study of virus fine morphological structure, isolation and purification of virus antigen protein, and detailed studies of virus chemical composition and genetic material.
The sucrose density gradient ultrafast purification process in traditional virus vaccine purification processing requires high-cost large centrifugal devices. These devices with small sample loading, long treatment times, and poor removal of host cell DNA. Gel filtration chromatography, widely used in a vaccine purification process, is mild, repeatable and easy to industrialise. However its efficiency is low, and the effect of removing heteroprotein and DNA is not obvious.
VirCap® AF media is an affinity chromatography media designed to capture and moderate capsular virus purification stages. Specific adsorption of VirCap® AF media and target occurs by simulating the affinity between ligands and virus particles with capsular membranes. With unique high loading capacity, high flow rate and low back pressure, VirCap® AF media reduces the process cycle time and increases the yield, fully meeting the requirements of large-scale biopharmaceutical and vaccine production processes.
Particle Microstructure & Ligand Bonding Technology
Particle SEM Picture — Biopure AF porous microsphere morphology
Ligand Bonding Technology
Functional groups or ligand on traditional packing materials are distributed along their surface. This limits their ability to effectively interact with bio-molecules. GALAK developed technologies to build “tentacle structure” on its ion-exchange and affinity packing materials. Flexible tentacle structures minimize the steric hindrance between functional groups or ligand and target molecules, improving the binding capability of the target molecules. Compared to traditional packing materials, GALAK’s PS-DVB particles show more effective capture and higher recovery.
Application Scenarios
From vaccine development to structural virology — Biopure AF supports every stage of your purification workflow.
Impfstoff-Reinigung
High-quality vaccine development requires efficient, scalable virus capture. Biopure AF delivers one-step enrichment and purification for live and inactivated virus vaccines.
Virus Morphological Studies
Provides purified virus particles suitable for fine morphological structure analysis using electron microscopy and other advanced imaging techniques.
Antigen Protein Isolation
Isolation and purification of viral antigen proteins for downstream characterization, formulation, and quality control in biopharmaceutical manufacturing.
Genetic Material Research
Obtain highly pure virus preparations for detailed studies of viral chemical composition and genetic material, supporting genomic and proteomic analysis.
Ready to optimize your virus purification workflow?
Related Products & Complementary Solutions
Build a complete purification workflow with Biovanix resins, columns, and systems.
Chromatography Resins & Media
Resin Family
InertShell Kern-Schale-Harz
PMMA core-shell technology designed for inactivated virus flow-through purification. Complements Biopure AF in a capture-and-polish workflow.
Resin Family
Supermacroporous Polymer Microspheres
Ultra-large pore polymer microspheres delivering 10× binding capacity for challenging separations of very large biomolecules and viruses.
Resin Family
PSDVB Chromatographiemedien
Polystyrene-divinylbenzene-based chromatography media with customizable pore sizes from 100 nm to 400 nm for diverse separation needs.
Resin Family
PMMA Chromatographiemedien
Polymethyl methacrylate-based media offering excellent biocompatibility and tailored surface chemistry for specific purification applications.
Corollary Equipment
Optimized chromatography columns designed to pair with Biopure AF resin.
Temperature-controlled chromatography with double-layer glass design for precise process conditions during virus purification.
Reliable low-pressure glass column for laboratory and pilot-scale virus purification with Biopure AF perfusion resin.
Frequently Asked Questions
Everything you need to know about Biopure AF perfusion chromatography resin.
What is perfusion chromatography and how does it differ from gel filtration?
Perfusion chromatography uses particles with large throughpores (3000–6000Å) that allow part of the mobile phase to flow directly through the microsphere, creating convective mass transfer. Traditional gel filtration relies entirely on diffusion to transport biomolecules into pores. This convective mechanism enables perfusion resins to maintain high resolution at 2–3× the throughput of conventional fast-flow gels.
How does the throughpore / porous structure enable high flow rate?
The large throughpores (3000–6000Å) allow part of the mobile phase to flow through the particle, quickly carrying biomolecules to smaller diffusive pores where binding occurs. This reduces the diffusion path and mass transfer resistance. Combined with flexible tentacle structures, the resin achieves linear flow rates up to 1000 cm/h at low backpressure without sacrificing binding capacity.
What are the advantages of the tentacle structure?
The flexible tentacle structure extends functional groups or ligands away from the particle surface on polymer chains, minimizing steric hindrance between the ligand and target molecules. This improves ligand accessibility and binding capacity, resulting in more effective capture and higher total recovery compared to traditional surface-bonded media.
What particle size and pore size ranges are available?
Biopure AF resin features 70 µm particle size with 3000–6000Å throughpores. The broader Biovanix PSDVB and PMMA platform also offers 45–90 µm particle sizes and pore sizes from 100 nm to 400 nm for customized separation needs.
What is the dynamic binding capacity?
Biopure AF has a dynamic binding capacity (DBC) of 100 mg lysozyme per ml of resin, maintained even at high flow rates thanks to the efficient convective mass transfer through the throughpore structure.
What flow rate and backpressure can I expect?
Biopure AF supports linear flow rates up to 1000 cm/h at 20°C with buffer viscosity equivalent to water, at less than 3 bar (43.5 psi) backpressure with a 20 cm column bed height. The rigid PS/DVB skeleton ensures minimal shrinkage or expansion during mobile phase changes.
What is the pH operating range and chemical stability?
Biopure AF is stable across the full pH range of 1–14. The rigid polymeric PS/DVB particle backbone coated with a proprietary hydrophilic polymer provides excellent chemical stability, tolerating 0.5–1M NaOH, organic solvents, and high-salt solvents for cleaning and sanitization.
Can Biopure AF be used for both live and inactivated virus purification?
Yes. Biopure AF features high selectivity with similar affinity to both live and inactivated capsular viruses. The closed-column operation ensures safety and sterility throughout the purification process.
What are the CIP and storage conditions?
Clean-in-Place (CIP) conditions include 0.5–1M NaOH, organic solvents, and high-salt solvents. Storage conditions are 2–8°C in 20% ethanol (EtOH).
Which viruses and antigens has this resin been applied to?
Biopure AF has been applied to purify Rabies, Influenza, Japanese Encephalitis, Feline Leukemia, Feline Herpes, Feline Calicivirus, Respiratory Syncytial Virus, Human Herpes Simplex, and Human Measles viruses, as well as Human Parainfluenza. Viral and microbial antigens include Herpes Simplex gA and gB Glycoprotein Subunits, Hepatitis B Surface Antigen, Filamentous Hemagglutinin from B. pertussis, and Leucocytosis Promoting Factor Hemagglutinin. A validated one-step purification protocol for live attenuated rabies virus vaccine is available.
How does Biopure AF relate to InertShell product lines?
Biopure AF is part of the VirCap series as a perfusion affinity resin specifically designed for capsular virus capture. InertShell is a separate PMMA core-shell technology product optimized for inactivated virus flow-through purification. The two product lines can be used together in an integrated capture-and-polish workflow.
What chromatography columns and equipment pair well with Biopure AF resin?
Biopure AF is compatible with Biovanix Jacketed Double-layer Glass Columns, Low-pressure Chromatography Columns, Isoplex HPLC-Säulen-Packungssystem, stainless steel column tubes, and protective pre-columns. Contact Biovanix for personalized equipment recommendations based on your scale and process requirements.
Optimize Your Virus Purification Process
Contact Biovanix to discuss your specific application, request sample quantities, or get a detailed quote for Biopure AF perfusion chromatography resin.
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