Affinity Phases Selectivity Affinity phases offer the highest specificity and selectivity in biomolecular separations and purifications. Purifications up to several orders of magnitude can be achieved in a single step. Affinity separations can often remove contaminants difficult to eliminate using conventional chromatographic procedures. Mechanism The basis of affinity chromatography is a ‘lock and key’ type

How to choose proper silica HPLC column? The selection of silica HPLC columns Despite its porosity, spherical porous HPLC silica exhibits a high mechanical strength compared with other materials. Additionally, it is readily chemically modified. A wide range of porous silica is available for normal-phase HPLC, characterized by surface area, pore size, and particle size

How to choose reversed-phase HPLC Columns? Different bonded phases for reversed-phase separations exhibit different combinations of possible solute-stationary phase interactions. For bonded phase, the variety of separation mechanisms will influence the overall selectivity. This predominance depend on the properties of the analyte, and the applied chromatographic conditions. The most significant interactions between solute and stationary phases

Brief Introduction of Liquid Chromatography The history of Liquid Chromatography (LC) began in the early 20th century. In 1906, a Russian botanist Mikhail Tswett invented LC in order to separate various plant pigments. He injected the plant extract and petroleum ether through a glass column packed with calcium carbonate. Since this was done on a