Core-Shell Technology for Virus Purification

InertShell Kern-Schale-Chromatographie-Harz

Für Adenoviridae ADV Reinigung

Biovanix InertShell Chromatographieharz is a revolutionary core-shell technology-based resin designed for the purification of viruses and large biomolecules. Combining size-exclusion separation with binding chromatography, this advanced resin efficiently captures and isolates large biomolecules while allowing smaller contaminants to pass through and bind within the core.

This dual functionality ensures high-purity outcomes in downstream processing.

20 mg/mL

BSA Binding Capacity

≤1.0 MPa

Maximaler Druck

pH 3–13

Wide pH Stability

30+

Reuse Cycles

How It Works

Dual-Function Core-Shell Architecture

Combining size-exclusion and binding chromatography in a single resin for unmatched purification efficiency.

Size-Exclusion + Binding in One Step

The outer shell is hydrophilic and neutral with 50-100 nm pores. Large biomolecules (e.g., inactivated viruses) are too large to penetrate the shell and are collected directly in the flow-through fraction (FT mode).

Smaller contaminants (<700 kDa) diffuse through the shell into the macroporous core (200-500 nm), where octylamine ligands provide both anion exchange and hydrophobic interaction to capture and retain impurities.

Key advantage: The thinner shell layer (0.5-1.0 μm vs. competitor’s 5 μm) enables faster mass transfer of impurities to the core for capture, resulting in higher yield of impurity removal and more efficient CIP cleaning.

Produktmerkmale

Designed for Demanding Biopurification

Four key capabilities that set InertShell apart in virus and biomolecule downstream processing.

🔬 Core-Shell Technology

Doppelte Funktionalität: Führt gleichzeitig Größenausschlusschromatographie und Affinitätschromatographie durch.
Effiziente Trennung: Große Biomoleküle (z.B. inaktivierte Viren) werden in der Durchflussfraktion (FT-Modus) gesammelt, während Verunreinigungen (<700 kDa) an die internen Liganden binden.
Optimiertes Design Die äußere Hülle ist hydrophil und neutral, was unerwünschte Wechselwirkungen verhindert und hohe Flussraten gewährleistet.

⚗️ Advanced Material Composition

Polymer-Basis: Hergestellt aus polymeren Methacrylat-Mikrokugeln für robuste Haltbarkeit und chemische Stabilität.
Aktiver Ligand: Der Octylamin-Ligand im Kern bietet sowohl Anionenaustausch- als auch hydrophobe Wechselwirkungen.
Poröse Struktur Die Schale hat eine Porengröße von 50-100 nm, während der Kern von 200-500 nm reicht, was eine effiziente Trennung nach Molekülgröße gewährleistet.

✨ High Purity and Efficiency

Selektive Erfassung Entfernt effektiv Verunreinigungen wie Wirtzellproteine, DNA-Fragmente, Endotoxine und Albumin.
Hohe Kapazität: Entwickelt für die groß angelegte Reinigung mit hoher Effizienz und Skalierbarkeit.

🛡️ Compliance and Safety

Nicht von Tieren abgeleitet Manufactured using synthetic processes, ensuring compliance with regulatory standards and ethical requirements.
Stabile Leistung Zuverlässig über verschiedene Produktionsmaßstäbe hinweg, sorgt für konsistente Ergebnisse.

Technical Specifications

Complete Product Specifications

Detailed technical data for method development, validation, and process integration.

Charakteristisch	Beschreibung
Support-Matrix	Vernetztes Poly(styrol-Divinylbenzol)
Durchschnittliche Partikelgröße	50–150 μm
Durchschnittliche Porengröße	200 nm (core); 50-100 nm (shell)
Oberflächenfunktionalität	Poly(dT) 25mer mit proprietärem Linker
Ligandenkonzentration	0,10–0,20 mmol/ml
Mechanische Beständigkeit	70 bar (1.000 psi; 7 MPa)
Thermische Stabilität	Allows sample denaturing at 65°C if needed
pH-Bereich	2-13
Bereich der Ionenstärke	0 bis 5 M, alle gängigen Salze
Chemische Beständigkeit	Common agents for mRNA purification, include 0.5 M NaOH, 2 M MgCl₂, 20 mM EDTA. Water, 0 to 100% alcohol, acetonitrile, 2 M acetic acid, 1 M HCl, and other common organic solvents
Lagerung	18-20% Ethanol

Competitive Edge

Biovanix InertShell vs. Competitor 700

Direct performance comparison demonstrates clear advantages across key parameters.

	Biovanix Inert Shell	Mitbewerber 700
Matrix	Polyacrylat	Hochvernetzte Agarose
Ligand	Octylamin	Octylamin
Durchschnittliche Partikelgröße	50–150 μm	50–150 μm
Dichte des Liganden	0,10–0,20 mmol/ml	0,04-0,085 mmol/ml
Bindekapazität	20 mg BSA/ml Harz	12 mg BSA/ml Harz
Betriebsdruck	≤1.0 MPa	≤0,3 MPa
Betriebsdurchflussrate	100-600 cm/h	100-600 cm/h
pH-Stabilität	3-13	3-13
Temperatur	4-30℃	4-30℃
Chemische Stabilität	Alle gängigen wässrigen Puffer, 1 M Natriumhydroxid (NaOH)², 6 M Guanidinhydrochlorid, 30% Isopropanol und 70% Ethanol.
Lagerung	20% Ethanol bei 4 °C bis 25 °C

Die dynamische Bindungskapazität wurde bei einem Durchbruch von 5% mit einer Durchflussrate von 76 cm/h auf Säulen mit den Abmessungen φ10×13 mm und einem Volumen von 1 mL gemessen. Als Puffer diente eine Lösung aus 1,0 mg/mL BSA und 50 mM NaCl, pH 0.
Keine signifikanten Änderungen der ionischen Kapazität und des Kohlenstoffgehalts nach einer Woche Lagerung in 1 M NaOH bei 25°C.

2.5×

Higher Ligand Density

0.10-0.20 vs. 0.04-0.085 mmol/mL

1.67×

Higher Binding Capacity

20 vs. 12 mg BSA/mL resin

3.3×

Higher Pressure Tolerance

1.0 vs. 0.3 MPa

Thinner Shell, Faster Mass Transfer

Compared with Competitor 700, the thickness of the core in Biovanix InertShell (0.5-1.0 μm) is significantly smaller than Competitor 700 (5 μm). This thinner shell is conducive to the rapid mass transfer of impure proteins to the medium core for capture, including host cell proteins, DNA fragments, endotoxin, and serum. Biovanix InertShell delivers a higher yield of impurity removal. With the macroporous structure (200-500 nm) of the core, InertShell can quickly remove captured impurities during CIP, and demonstrates a longer service life — in animal vaccine studies, consistent performance for more than 30 reuse cycles.

Vorteile auf einen Blick

Why Biovanix InertShell Resin

Purpose-built for high-purity virus and biomolecule downstream processing.

⚡

Hoher Wirkungsgrad

Schnelle und effiziente mRNA-Aufreinigung.

📈

Skalierbarer Prozess

Leicht anpassbar an die Großserienfertigung.

🛡️

Einhaltung von Vorschriften

Synthetisches Herstellungsverfahren ohne Tierversuche.

💰

Kostengünstig

Vereinfachte Arbeitsabläufe reduzieren die betriebliche Komplexität und die Kosten.

🔄

Long Service Life

30+ reuse cycles with consistent performance.

Anwendungen

Built for the Future of Biopharmaceuticals

From vaccine production to gene therapy, InertShell supports your most critical purification workflows.

Virusreinigung

Effektive Isolierung inaktivierter Viren für Impfstoffe und Gentherapien.

Große Biomolekül-Trennung

Geeignet zur Reinigung großer Proteine und Komplexe.

Entfernung von Wirtzellproteinen

Effiziente Eliminierung von Wirtzellproteinen und anderen Verunreinigungen.

Impfstoffproduktion

Optimierte nachgelagerte Prozesse für mRNA-Impfstoffe und Impfstoffe auf Vektorviralbasis.

Gentherapie

Skalierbare Produktion von viralen Vektoren für Gentherapieanwendungen.

Why Choose Biovanix

Your Trusted Partner in Chromatography

① Enhanced Purity

Effiziente Entfernung von Verunreinigungen zur Erzielung ultrahoher Reinheit bei der Virus- und Biomolekülaufreinigung.

② Dual Mode Operation

Kombiniert Größenausschluss- und Affinitätschromatographie für vielseitige und präzise Kontrolle.

③ Scalable Solution

Ideal für sowohl Laborma.

④ Cost-Effective

Vereinfachte Prozessabläufe und hohe Effizienz reduzieren die Betriebskosten und verbessern die Produktivität.

⑤ Regulatory Compliance

Nicht-tierische und synthetische Herstellung gewährleisten die Einhaltung globaler biopharmazeutischer Standards.

Frequently Asked Questions

InertShell Resin FAQ

Common questions about core-shell chromatography for virus and biomolecule purification.

What is InertShell Core-Shell Chromatography Resin and how does it work?

Biovanix InertShell is a core-shell chromatography resin designed for the purification of viruses and large biomolecules. It combines size-exclusion separation with binding chromatography in a single step. The outer shell is hydrophilic and neutral with 50-100 nm pores, while the inner core features macropores of 200-500 nm functionalized with octylamine ligands. Large biomolecules like inactivated viruses pass through in the flow-through fraction, while smaller contaminants (<700 kDa) including host cell proteins, DNA fragments, and endotoxins bind to the internal ligands.

What is the difference between core-shell and fully porous chromatography resins?

Core-shell resins feature a non-porous solid core surrounded by a thin porous outer shell. This architecture reduces mass transfer resistance compared to fully porous particles, enabling faster flow rates with lower backpressure. The reduced diffusion path means analytes spend less time diffusing in and out of pores, resulting in sharper peaks, higher resolution, and shorter processing times. Biovanix InertShell further enhances this by using a polymeric methacrylate base rather than silica, providing superior chemical stability across pH 3-13.

What applications is InertShell resin best suited for?

InertShell resin is optimized for five key applications: (1) Virus purification — efficient isolation of inactivated viruses for vaccines and gene therapies; (2) Large biomolecule separation — purification of large proteins and complexes; (3) Host cell protein (HCP) removal — efficient elimination of HCPs and other process impurities; (4) Vaccine production — streamlined downstream processing for mRNA vaccines and viral vector-based vaccines; (5) Gene therapy — scalable production of viral vectors. It is particularly suited for adenovirus (ADV) purification.

How does InertShell compare to competing core-shell resins?

Compared to Competitor 700 (agarose-based), Biovanix InertShell offers several advantages: (1) 1.67× higher binding capacity (20 mg BSA/mL vs. 12 mg BSA/mL); (2) 3.3× higher operational pressure tolerance (1.0 MPa vs. 0.3 MPa); (3) 2.5× higher ligand density (0.10-0.20 mmol/mL vs. 0.04-0.085 mmol/mL); (4) Thinner shell layer (0.5-1.0 μm vs. 5 μm) enabling faster mass transfer of impurities to the core for capture; (5) Polymeric methacrylate base provides greater chemical stability than agarose; (6) Demonstrated reusable performance of 30+ cycles with minimal property changes in animal vaccine applications.

What is the recommended flow rate for InertShell resin?

Biovanix InertShell supports operational flow rates of 100-600 cm/h at pressures up to 1.0 MPa (approximately 10 bar). The rigid polymeric methacrylate matrix maintains structural integrity even at elevated flow rates. Users should adjust flow rate depending on the specific application and performance requirements, while ensuring the upper pressure limitation is not exceeded. The core-shell architecture ensures efficient mass transfer even at high linear velocities.

Can InertShell resin be reused, and how do I clean it?

Yes, InertShell resin is designed for multiple reuse cycles. The resin demonstrates excellent chemical stability and can withstand cleaning with 1 M NaOH (no significant changes in ionic capacity and carbon content after 1 week storage in 1 M NaOH at 25°C), 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol. The macroporous core structure (200-500 nm) enables rapid removal of captured impurities during CIP. In animal vaccine purification studies, the resin has been demonstrated to maintain consistent performance over 30+ reuse cycles.

What buffer and storage conditions are required?

InertShell resin is compatible with all commonly used aqueous buffers, 1 M NaOH, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol. It operates across a pH range of 3-13 and ionic strength range of 0 to 5 M. Operating temperature range is 4-30°C. For storage, keep the resin in 20% ethanol at 4°C to 25°C. Do not expose to strong oxidizers (hypochlorite), oxidizing acids (nitric acid), strong reducing agents (sulfite), acetone, THF, or benzyl alcohol.

How does InertShell achieve dual functionality in size-exclusion and binding chromatography?

InertShell achieves dual functionality through its unique core-shell architecture. The outer shell is hydrophilic and neutral with small pores (50-100 nm), which prevents large biomolecules like viruses from entering and interacting with the surface. These large molecules are collected in the flow-through fraction (FT mode). Meanwhile, the inner core has large macropores (200-500 nm) functionalized with octylamine ligands that provide both anion exchange and hydrophobic interaction capabilities, capturing smaller contaminants (<700 kDa) including host cell proteins, DNA fragments, endotoxins, and albumin.

Is InertShell resin suitable for GMP manufacturing?

Yes, InertShell resin is manufactured using synthetic processes and is non-animal derived, ensuring compliance with regulatory standards and ethical requirements. This makes it suitable for use in GMP manufacturing environments for vaccine production and gene therapy applications. The consistent performance across various production scales and the demonstrated reusability (30+ cycles) support cost-effective and compliant large-scale biopharmaceutical manufacturing.

What particle size does InertShell use and why does it matter?

InertShell resin uses particles with an average size of 50-150 μm. The core-shell design within each particle is key: the thin outer shell (0.5-1.0 μm thickness) with small pores creates the size-exclusion effect for large biomolecules, while the macroporous core provides high-capacity binding for smaller impurities. This dual-size architecture within each particle enables the simultaneous size-based separation and affinity-based capture that distinguishes InertShell from conventional single-mechanism resins.

How does InertShell compare to agarose-based resins for virus purification?

InertShell’s polymeric methacrylate base offers significant advantages over traditional agarose-based resins: (1) Higher mechanical strength — supports pressures up to 1.0 MPa vs. 0.3 MPa for agarose, enabling faster processing; (2) Wider chemical compatibility — stable with NaOH, guanidine hydrochloride, organic solvents; (3) Higher binding capacity — 20 mg BSA/mL vs. 12 mg BSA/mL; (4) Higher ligand density — 0.10-0.20 mmol/mL vs. 0.04-0.085 mmol/mL; (5) Longer service life — demonstrated 30+ reuse cycles with consistent performance; (6) Faster mass transfer — thinner shell layer (0.5-1.0 μm vs. 5 μm) allows quicker impurity capture and CIP cleaning.

What impurities can InertShell resin remove from my sample?

InertShell resin effectively removes a broad range of process-related impurities while retaining your target large biomolecules in the flow-through: (1) Host cell proteins (HCPs); (2) DNA fragments; (3) Endotoxins; (4) Albumin; (5) Other contaminants smaller than 700 kDa. The octylamine ligand in the core provides both anion exchange and hydrophobic interaction mechanisms, enabling multi-modal impurity capture. This comprehensive impurity removal in a single step significantly simplifies downstream processing workflows.

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Ready to Transform Your Virus Purification Workflow?

Contact our application scientists for personalized technical support, sample testing, or volume pricing on InertShell resin.