The Selection For HPLC Column By Column Dimension

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Column dimension (I.D. × length in mm)	Aplicación	Reason
4.6/10/20×10	Guard-column	Protection from mechanical contamination Sample contaminated to low extent
4.6×30	Precolumn	High capacity precolumn
2.1/3.0/4.0/4.6×30 2.1/3.0/4.0/4.6×50	Method development Rapid HPLC and UHPLC (if pressure stable)	Short retention time Rapid equilibration Low solvent consumption (small I.D.) Low pressure drop
2.1×100 2.1/3.0×150	High detection sensitivity (mass selectivity)	Semi-micro column for low injection volumes and low peak Low solvent consumption
4.6×100 4.0/4.6×125 4.0/4.6×150	Standard column	Adequate performance for most applications (average performance 8000 – 10000 N/column)
2.1/3.0×250	High detection sensitivity High performance separation	Semi-micro column for low injection volumes and low peak Low solvent consumption For complex samples
4.6×250	High robustness/higher tolerance to injection volume	For very complex samples
10×150; 10×250	Semi-preparative	For mg quantities of pure substance on lab scale
20/21.2/30/50×150 20/21.2/30/50×250	Preparative	For g quantities of pure substance
BioVanix provide customized column size based on your needs