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Colonnes HPLC préemballées de précision pour des performances de séparation inégalées

High-Performance Liquid Chromatography (HPLC) columns are essential tools in modern analytical chemistry, widely used in pharmaceutical development, biomedical research, food safety testing, and environmental monitoring. HPLC columns achieve the efficient separation of complex mixtures by exploiting the differences in the distribution of solutes between the stationary phase and the mobile phase.

10,000

Max Pressure (psi)

2.1–50

Column ID Range (mm)

pH 2–12

Stable Operating Range

20+

Column Types Available

Technical Advantages

Engineered for Reproducibility, High Pressure Resistance & Superior Peak Resolution

Every Biovanix HPLC column is designed to deliver consistent, high-quality separations across a wide range of analytical conditions.

Types de phases stationnaires

Common stationary phases include silica, polymers, and chemically modified materials such as C18, C8, NH₂, etc. Different stationary phases are suitable for the separation of compounds with varying polarities.

Taille des particules et taille des pores

Particle sizes typically range from 1.8 µm to 10 µm, while pore sizes vary depending on the application, such as 100 Å, 200 Å, and 300 Å. Smaller particles can enhance separation efficiency but may require higher operating pressures.

Longueur et diamètre intérieur de la colonne

The selection of column length and inner diameter depends on the separation requirements. Longer columns generally provide higher separation efficiency.

Stabilité du pH

Some HPLC columns can operate stably over a broad pH range (e.g., pH 2-12), enabling flexible method development with aggressive mobile phases.

Catalogue des produits

Comprehensive HPLC Column Portfolio

Explore our full range of prepacked chromatography columns organized by application category. Click any product to learn more.

C18 Colonne et vrac

C8 Colonne et vrac

C4 Colonne et vrac

Phényl Colonne et vrac

SiO2 en colonne et en vrac

NH2/Amino Colonne et vrac

CN/Cyano Colonne et vrac

Colonne de diol et vrac

Colonne amide

Colonne HILIC

Colonne échangeuse d'ions

Colonne de sucre

Chronique SEC

Colonne HIC

Colonne d'ADN

Colonne chirale

Colonne de protéines A

Colonne d'analyse HbA1c

Tubes pour colonnes HPLC

Tube à colonne en PEEK

Colonne de garde

Filtres en ligne

Applications

Trusted Across Industries

From drug development to environmental protection, Biovanix HPLC columns deliver reliable performance where it matters most.

Développement pharmaceutique

Utilisé pour la séparation, la purification et le contrôle de la qualité des composants des médicaments afin de garantir la sécurité et l'efficacité des produits pharmaceutiques.

Recherche biomédicale

Analyse des biomolécules (telles que les protéines et les peptides) et des métabolites pour soutenir la recherche en sciences de la vie.

Tests de sécurité alimentaire

Détecte les substances nocives telles que les additifs et les résidus de pesticides dans les aliments.

Surveillance de l'environnement

Contrôle des polluants organiques et des résidus de pesticides dans l'eau et le sol.

Selection Guide

How to Choose the Right HPLC Column

Selecting the optimal column dimension and stationary phase is critical for achieving the best separation results.

En fonction de l'échelle et/ou de l'efficacité de la séparation requise, le tableau ci-dessous peut vous aider à choisir une colonne dont le diamètre intérieur (D.I.) et la longueur sont les plus appropriés à vos besoins.

Quelle est la longueur de colonne la mieux adaptée à mes besoins ?

Si vous voulez maximiser la vitesse de votre application → 20 mm à 75 mm
Si vous souhaitez un équilibre entre résolution et vitesse → 100 mm
Si vous souhaitez la meilleure résolution possible → 150 mm
Également disponible en dimensions analytiques et semi-prep.

Les colonnes USP (United States Pharmacopeia) sont des colonnes de chromatographie liquide à haute performance (HPLC) conçues pour répondre aux normes strictes établies par l'USP, garantissant la cohérence, la fiabilité et la conformité des procédures analytiques. Ces colonnes sont largement utilisées dans les applications pharmaceutiques, environnementales et de sécurité alimentaire pour fournir des résultats précis et reproductibles.

Why Biovanix

Why Choose Biovanix HPLC Columns?

Every column is engineered and manufactured to the highest standards, backed by our commitment to quality and technical support.

01

Stabilité à haute pression

Withstands up to 10 000 psi for UHPLC applications

Materials: 316L stainless steel or biocompatible PEEK

02

Résolution de crête exceptionnelle

Mirror-polished inner surface (Ra ≤0.2 μm) minimizes band broadening, delivering sharp, well-defined peaks for precise quantification.

03

Options personnalisables

Dimensions de la colonne : 2,1-50 mm ID | 20-500 mm longueur

Packing materials: C18, C8, HILIC, ion exchange, affinity resins

04

Large gamme de pH

Compatible with aggressive mobile phases across a broad pH range, enabling flexible method development for diverse analytes.

05

Conception à faible volume mort

Ensures minimal sample loss and high reproducibility, critical for trace-level analysis and quantitative accuracy.

Need Custom Specifications?

Our engineering team can tailor column dimensions, stationary phases, and end fittings to your exact requirements.

FAQ

Frequently Asked Questions

Everything you need to know about Biovanix prepacked HPLC columns.

What are prepacked HPLC columns and how do they work?

Prepacked HPLC columns are factory-filled, ready-to-use chromatographic columns where the stationary phase (typically chemically modified silica particles) is uniformly packed under high pressure into stainless steel or PEEK tubing.

They work by exploiting differential interactions between analytes, the stationary phase, and the mobile phase. Sample components partition based on hydrophobicity, polarity, charge, or size as the mobile phase is pumped through the column at high pressure. Compounds with stronger affinity for the stationary phase are retained longer, enabling efficient separation, identification, and quantification.

What types of HPLC columns does Biovanix offer?

Biovanix offers six major categories:

Reversed-Phase Columns — C18, C18H, C18L, C18AQ, C18BS, C8, C4-300, Phenyl
Normal-Phase Columns — SiO₂, NH₂, CN, Diol, Amide
Small-Molecule Columns — HILIC, Ion Exchange (SCX/SAX), Sugar Analysis
Biological Columns — SEC (SEC-120/150/300/500/1000), HIC, DNA
Specialty Columns — Chiral (8 types), Protein A, HbA1c
Consommables — SS/PEEK column tubing, guard columns, inline filters, frits, fittings

What is the maximum operating pressure for Biovanix HPLC columns?

Taille des particules	Max. Pression
10 μm	4,000 psi
5 μm	5,000 psi
3 μm	6,000 psi
SEC (3 μm)	2,500 psi
SEC (5 μm)	1 500 psi
UHPLC applications	Up to 10,000 psi

Temperature limits: 60 °C for reversed-phase columns; 40 °C for SEC columns.

Can I customize HPLC column dimensions and packing materials?

Yes. Biovanix supports full customization:

Inner diameter: 2.1–50 mm (10 standard sizes); non-standard sizes available upon request.
Column length: 20–500 mm (8 standard lengths); custom lengths accepted.
Packing materials: C18, C8, Phenyl, NH₂, CN, Diol, Bare Silica and more (particle sizes 3–50 μm; pore sizes 100/120/300 Å). Special bonded phases are available upon request.
Matériel: 316L stainless steel or PEEK tubing; multiple frit types; custom end fittings and seals.

Are Biovanix HPLC columns suitable for pharmaceutical quality control?

Yes. Key qualifications include:

Columns carry recognized USP codes (L1, L3, L7, L8, L9, L10, L11, L14), enabling direct compliance with pharmacopeial methods.
Ultra-pure silica with metal ions < 20 ppm ensures minimal peak tailing for basic compounds.
Proprietary bonding chemistry with deep endcapping delivers low silanol activity and excellent batch-to-batch reproducibility.
pH stability from 2–8 (up to 1–12 for C18BS) covers diverse mobile phase conditions.
Manufacturing follows ISO 9001 quality standards.
Applications explicitly include drug substance characterization, impurity profiling, dissolution testing, and content uniformity analysis.

How do I select the right HPLC column for my application?

Select based on four key parameters:

① Separation mode — Match the analyte properties to the column chemistry:

Hydrophobic compounds → C18 / C8 (reversed-phase)
Aromatic compounds → Phenyl
Polar compounds → HILIC / Amide
Sugars → Sugar-10H / NH₂
Proteins/antibodies → SEC-300 / C4-300
Enantiomers → Chiral columns

② Column dimensions — Choose by scale:

Analytical QC: 2.1–4.6 mm ID × 100–250 mm length
Semi-preparative: 7.8–10 mm ID
Preparative: 20–50 mm ID

③ Particle size — Balance resolution and pressure:

3 μm: highest resolution (pharmacopeial methods)
5 μm: optimal balance (most common)
10 μm: low pressure, scalable to preparative

④ Pore size — Match to molecular weight:

100 Å: small molecules (< 3 kDa)
300 Å: peptides and proteins
SEC series: size-based separation of biomolecules

Ready to Optimize Your Chromatography?

Get detailed pricing, technical specifications, and custom column consultation from our experts.

Colonne CLHP à phase inversée	Colonne HPLC en phase normale	Colonne HPLC pour petites molécules	Colonne CLHP biologique	Colonne HPLC spéciale	Consommables
C18 Colonne	Colonne SiO2	Colonne HILIC	Chronique SEC	Colonne chirale	Tubes de colonne
Colonne C8	Colonne CN	Colonne échangeuse d'ions	Colonne HIC	Colonne de protéines A	Colonne PEEK
Colonne C4	Colonne NH2	Colonne de sucre	Colonne d'ADN	Colonne HbA1C	Colonne de garde
Colonne phényle	Colonne à diols				Filtres en ligne
	Colonne amide				Colonne Ghost Peak